################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BanI BbeI BbrPI BbvCI BbvI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MseI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I TseI Tsp45I TspRI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 292 CGCG CG^CG AciI 723 CCGC CCGC(-3/-1) AcsI 107 GAATTC [GA]^AATT[CT] AfaI 953 GTAC GT^AC AhdI 818 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 107 GAATTC [GA]^AATT[CT] AspEI 818 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 52 GGACC G^G[AT]CC BamHI 71 GGATCC G^GATCC BanII 287 GGGCTC G[GA]GC[CT]^C BcgI 475 CGAAGCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 20 TGATCA T^GATCA BfaI 283 CTAG C^TAG BmyI 287 GGGCTC G[AGT]GC[ACT]^C BpmI 82 CTGGAG CTGGAG(16/14) BsgI 878 CTGCAC GTGCAG(16/14) BsmAI 826 GTCTC GTCTC(1/5) Bsp1286I 287 GGGCTC G[AGT]GC[ACT]^C BsrDI 663 CATTGC GCAATG(2/0) BstBI 473 TTCGAA TT^CGAA BstUI 292 CGCG CG^CG BstYI 71 GGATCC [GA]^GATC[CT] Cfr13I 52 GGACC G^G[ACGT]CC DraII 51 AGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 818 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 51 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 107 GAATTC G^AATTC EcoRV 595 GATATC GAT^ATC FauI 722 CCCGC CCCGC(4/6) FbaI 20 TGATCA T^GATCA HindIII 477 AAGCTT A^AGCTT MaeI 283 CTAG C^TAG MaeIII 41 GTTAC ^GT[ACGT]AC MflI 71 GGATCC [GA]^GATC[CT] MspA1I 248 CAGCTG C[AC]G^C[GT]G MvnI 292 CGCG CG^CG NruI 291 TCGCGA TCG^CGA NspI 514 ACATGC [GA]CATG^[CT] NspV 473 TTCGAA TT^CGAA PleI 432 GACTC GAGTC(4/5) PpuMI 51 AGGACCC [GA]G^G[AT]CC[CT] PstI 528 CTGCAG CTGCA^G PvuII 248 CAGCTG CAG^CTG RsaI 953 GTAC GT^AC Sau96I 52 GGACC G^G[ACGT]CC SfcI 528 CTGCAG C^T[GA][CT]AG SfuI 473 TTCGAA TT^CGAA SmlI 411 CTTGAG C^T[CT][GA]AG StuI 78 AGGCCT AGG^CCT TaqI 474 TCGA T^CGA TthHB8I 474 TCGA T^CGA XcmI 93 CCATGTGTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 71 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 72 GATCC GGATC(4/5) 71 GGATC BalI 221 TGGCCA TGG^CCA 371 TGGCCA BbsI 906 GAAGAC GAAGAC(2/6) 185 GTCTTC BpuAI 906 GAAGAC GAAGAC(2/6) 185 GTCTTC BsiYI 75 CCCAGGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 165 CCTCATTGTGG BslI 75 CCCAGGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 165 CCTCATTGTGG BspHI 189 TCATGA T^CATGA 635 TCATGA BspMI 783 ACCTGC ACCTGC(4/8) 876 ACCTGC BstXI 362 CCATTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 807 CCATCGCTTTGG DsaI 374 CCATGG C^C[GA][CT]GG 890 CCATGG EaeI 221 TGGCCA [CT]^GGCC[GA] 371 TGGCCA EcoT14I 374 CCATGG C^C[AT][AT]GG 890 CCATGG MluNI 221 TGGCCA TGG^CCA 371 TGGCCA MscI 221 TGGCCA TGG^CCA 371 TGGCCA MwoI 530 GCAGGACACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 577 GCCCAGCTCGC NcoI 374 CCATGG C^CATGG 890 CCATGG NlaIV 52 GGACCC GG[ACGT]^[ACGT]CC 71 GGATCC RcaI 189 TCATGA T^CATGA 635 TCATGA StyI 374 CCATGG C^C[AT][AT]GG 890 CCATGG TfiI 355 GATTC G^A[AT]TC 657 GATTC Tsp509I 89 AATT ^AATT 108 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI BsaJI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII FokI HaeIII HinfI MboI MboII MnlI MslI MvaI NdeII NlaIII Sau3AI ScrFI SfaNI