(Circular) (Six-base) MAPSORT of: pFN21AE6062.seq Check: 8802 from: 1 to: 5665 pFN21AE6062 5665 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2797 2890 2797 Size: 93 5572 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3008 1937 Size: 1071 4594 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3728 5112 5380 1632 Size: 2096 1384 268 1917 Fragments arranged by size: 2096 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5468 AflIII A'CryG_T Cuts at: 1203 4564 5541 1203 Size: 3361 977 1327 Fragments arranged by size: 3361 1327 977 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 2828 4394 2828 Size: 1566 4099 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2513 1184 Size: 1329 4336 AlwNI CAG_nnn'CTG Cuts at: 3408 4980 3408 Size: 1572 4093 ApaLI G'TGCA_C Cuts at: 3722 4878 3722 Size: 1156 4509 ApoI r'AATT_y Cuts at: 1493 2112 2974 3200 3352 5461 1493 Size: 619 862 226 152 2109 1697 Fragments arranged by size: 2109 1697 862 619 226 152 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2990 1870 Size: 73 1047 4545 Fragments arranged by size: 4545 1047 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2949 2982 2949 Size: 33 5632 BamHI G'GATC_C Cuts at: 2076 2995 3362 2076 Size: 919 367 4379 Fragments arranged by size: 4379 919 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 4441 618 Size: 325 217 627 1199 1455 1842 Fragments arranged by size: 1842 1455 1199 627 325 217 BanII G_rGCy'C Cuts at: 1391 2296 2984 1391 Size: 905 688 4072 Fragments arranged by size: 4072 905 688 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2183 2918 928 Size: 971 284 735 3675 Fragments arranged by size: 3675 971 735 284 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2469 2503 3856 3890 1903 Size: 34 50 34 448 34 1353 34 3678 Fragments arranged by size: 3678 1353 448 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3557 4767 3557 Size: 1210 4455 BclI T'GATC_A Cuts at: 1548 2025 1548 Size: 477 5188 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4276 136 Size: 107 122 71 631 605 2604 1525 Fragments arranged by size: 2604 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5660 5660 Size: 5665 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 3726 4882 1790 Size: 1936 1156 2573 Fragments arranged by size: 2573 1936 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5665 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3396 4354 442 Size: 416 53 83 399 24 1979 958 1753 Fragments arranged by size: 1979 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 4325 1239 Size: 869 2217 2579 Fragments arranged by size: 2579 2217 869 Bpu10I CC'TnA_GC Cuts at: 2039 2039 Size: 5665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2437 3790 4655 4953 2437 Size: 1353 865 298 3149 Fragments arranged by size: 3149 1353 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4328 882 Size: 617 2829 2219 Fragments arranged by size: 2829 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3456 493 Size: 711 2252 2702 Fragments arranged by size: 2702 2252 711 BsaBI GATnn'nnATC Cuts at: 5659 5659 Size: 5665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3472 3854 4489 275 Size: 53 83 186 936 1939 382 635 1451 Fragments arranged by size: 1939 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4098 4496 4770 4917 5328 4098 Size: 398 274 147 411 4435 Fragments arranged by size: 4435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5440 Fragments arranged by size: 5440 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5665 BseYI C'CCAG_C Cuts at: 2583 4868 5364 2583 Size: 2285 496 2884 Fragments arranged by size: 2884 2285 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2868 1163 Size: 573 1132 3960 Fragments arranged by size: 3960 1132 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3115 3876 4904 1540 Size: 275 186 1114 761 1028 2301 Fragments arranged by size: 2301 1114 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3726 3811 4882 3726 Size: 85 1071 4509 Fragments arranged by size: 4509 1071 85 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2296 2984 3726 3811 4882 1391 Size: 399 506 688 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 688 506 399 85 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 2194 2640 3554 5284 2194 Size: 446 914 1730 2575 Fragments arranged by size: 2575 1730 914 446 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2797 2890 3020 844 Size: 1953 93 130 3489 Fragments arranged by size: 3489 1953 130 93 BsrBI CCG'CTC Cuts at: 1394 1823 3552 1394 Size: 429 1729 3507 Fragments arranged by size: 3507 1729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2667 4160 4334 65 Size: 1183 1419 1493 174 1396 Fragments arranged by size: 1493 1419 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4309 4496 1700 Size: 110 2499 187 2869 Fragments arranged by size: 2869 2499 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5665 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5665 BssSI C'ACGA_G Cuts at: 3725 4737 3725 Size: 1012 4653 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5665 BstBI TT'CG_AA Cuts at: 2479 4469 2479 Size: 1990 3675 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstXI CCAn_nnnn'nTGG Cuts at: 2374 2819 2374 Size: 445 5220 BstYI r'GATC_y Cuts at: 1972 2076 2995 3362 3747 3764 5216 5302 5314 Size: 104 919 367 385 17 1452 86 12 Cuts at: 5314 5660 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 919 385 367 346 104 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2379 2895 513 Size: 552 1314 516 3283 Fragments arranged by size: 3283 1314 552 516 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2798 487 Size: 683 56 1572 3354 Fragments arranged by size: 3354 1572 683 56 BtsI GCAGTG_nn' Cuts at: 3181 3975 3995 3181 Size: 794 20 4851 Fragments arranged by size: 4851 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5630 DraI TTT'AAA Cuts at: 2969 2969 Size: 5665 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 2376 3112 Size: 54 1425 50 275 18 396 150 736 Cuts at: 3112 4001 5630 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 736 396 275 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3595 1353 Size: 2242 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4252 4626 4772 387 Size: 1064 138 2663 374 146 1280 Fragments arranged by size: 2663 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 3728 4325 5112 5380 1239 Size: 393 476 1620 597 787 268 1524 Fragments arranged by size: 1620 1524 787 597 476 393 268 EcoO109I rG'GnC_Cy Cuts at: 2057 2057 Size: 5665 EcoRI G'AATT_C Cuts at: 2112 2974 3352 2112 Size: 862 378 4425 Fragments arranged by size: 4425 862 378 EcoRV GAT'ATC Cuts at: 2602 2602 Size: 5665 FspI TGC'GCA Cuts at: 4171 4171 Size: 5665 HaeII r_GCGC'y Cuts at: 1678 4812 1678 Size: 3134 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2814 2846 4306 4338 4380 Size: 32 135 32 1242 32 1460 32 42 Cuts at: 4380 4412 1373 Size: 32 2626 Fragments arranged by size: 2626 1460 1242 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3009 3255 669 Size: 988 281 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 HindIII A'AGCT_T Cuts at: 748 2482 748 Size: 1734 3931 HpaI GTT'AAC Cuts at: 3255 3255 Size: 5665 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 5541 5541 Size: 5665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4779 4963 1305 Size: 3474 184 2007 Fragments arranged by size: 3474 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2228 2378 10 Size: 54 1425 343 396 150 3297 Fragments arranged by size: 3297 1425 396 343 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2372 2574 2763 2774 3623 3982 Size: 648 1011 195 202 189 11 849 359 Cuts at: 3982 4141 518 Size: 159 2042 Fragments arranged by size: 2042 1011 849 648 359 202 195 189 159 11 MspA1I CmG'CkG Cuts at: 1606 2255 3081 3758 4906 5151 1606 Size: 649 826 677 1148 245 2120 Fragments arranged by size: 2120 1148 826 677 649 245 NcoI C'CATG_G Cuts at: 513 1065 2379 2895 513 Size: 552 1314 516 3283 Fragments arranged by size: 3283 1314 552 516 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NruI TCG'CGA Cuts at: 2298 2298 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 2523 3023 4568 1132 Size: 97 1294 500 1545 2229 Fragments arranged by size: 2229 1545 1294 500 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5665 PciI A'CATG_T Cuts at: 4564 4564 Size: 5665 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5665 PfoI T'CCnGG_A Cuts at: 2053 2355 2053 Size: 302 5363 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3734 3766 5247 5279 3734 Size: 32 1481 32 4120 Fragments arranged by size: 4120 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2057 2057 Size: 5665 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5665 PsiI TTA'TAA Cuts at: 3235 3235 Size: 5665 PstI C_TGCA'G Cuts at: 830 2537 3017 830 Size: 1707 480 3478 Fragments arranged by size: 3478 1707 480 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 PvuII CAG'CTG Cuts at: 2255 2255 Size: 5665 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SbfI CC_TGCA'GG Cuts at: 3017 3017 Size: 5665 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SfcI C'TryA_G Cuts at: 826 1045 2533 3013 4148 4829 5020 826 Size: 219 1488 480 1135 681 191 1471 Fragments arranged by size: 1488 1471 1135 681 480 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2992 1872 Size: 1120 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2416 3769 4670 4932 820 Size: 197 926 473 1353 901 262 1553 Fragments arranged by size: 1553 1353 926 901 473 262 197 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 3023 1132 Size: 1891 3774 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 2085 1517 Size: 568 5097 StyI C'CwwG_G Cuts at: 513 1065 2379 2895 3104 513 Size: 552 1314 516 209 3074 Fragments arranged by size: 3074 1314 552 516 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3692 3709 3862 4047 4492 325 Size: 1255 2112 17 153 185 445 1498 Fragments arranged by size: 2112 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3911 5623 96 Size: 274 80 33 51 1443 1934 1712 138 Fragments arranged by size: 1934 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5625 5657 5625 Size: 32 5633 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2105 2105 Size: 5665 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2990 1870 Size: 1120 4545 XmnI GAAnn'nnTTC Cuts at: 3794 3794 Size: 5665 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI