################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 886 GTCTAC GT^[AC][GT]AC AciI 378 CCGC CCGC(-3/-1) AluI 314 AGCT AG^CT Aor51HI 170 AGCGCT AGC^GCT AspHI 278 GAGCAC G[AT]GC[AT]^C AvaII 828 GGTCC G^G[AT]CC AviII 680 TGCGCA TGC^GCA BamHI 10 GGATCC G^GATCC BanI 816 GGCACC G^G[CT][GA]CC BbsI 897 GAAGAC GAAGAC(2/6) BglI 700 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 310 GCTCAGC GC^T[ACGT]AGC BmyI 278 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 310 GCTCAGC GC^T[ACGT]AGC BpuAI 897 GAAGAC GAAGAC(2/6) BsaBI 93 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 141 CTGCAC GTGCAG(16/14) BsiHKAI 278 GAGCAC G[AT]GC[AT]^C BsmBI 624 CGTCTC CGTCTC(1/5) Bsp1286I 278 GAGCAC G[AGT]GC[ACT]^C BspHI 180 TCATGA T^CATGA BsrI 529 CCAGT ACTGG(1/-1) BssSI 281 CACGAG CACGAG(-5/-1) BstXI 798 CCATCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 310 GCTCAGC GC^T[ACGT]AGC DraII 722 AGGCCCT [GA]G^G[ACGT]CC[CT] Eco47III 170 AGCGCT AGC^GCT EcoNI 825 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 722 AGGCCCT [GA]G^G[ACGT]CC[CT] FspI 680 TGCGCA TGC^GCA HaeII 170 AGCGCT [GA]GCGC^[CT] HgaI 924 GACGC GACGC(5/10) HphI 422 TCACC GGTGA(8/7) MamI 93 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PaeR7I 343 CTCGAG C^TCGAG PshAI 223 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 180 TCATGA T^CATGA SapI 762 GCTCTTC GCTCTTC(1/4) SexAI 824 ACCTGGT A^CC[AT]GGT SmaI 132 CCCGGG CCC^GGG SmlI 343 CTCGAG C^T[CT][GA]AG Tsp45I 922 GTGAC ^GT[GC]AC XhoI 343 CTCGAG C^TCGAG XmaCI 132 CCCGGG C^CCGGG XmaI 132 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AvaI 132 CCCGGG C^[CT]CG[GA]G 343 CTCGAG BpmI 702 CTCCAG CTGGAG(16/14) 73 CTGGAG BsaI 229 GGTCTC GGTCTC(1/5) 453 GGTCTC BsoBI 132 CCCGGG C^[CT]CG[GA]G 343 CTCGAG Cfr13I 723 GGCCC G^G[ACGT]CC 828 GGTCC HapII 133 CCGG C^CGG 912 CCGG HpaII 133 CCGG C^CGG 912 CCGG MaeIII 922 GTGAC ^GT[ACGT]AC 938 GTTAC MseI 161 TTAA T^TAA 728 TTAA MslI 745 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 771 CACTCCCATG MspI 133 CCGG C^CGG 912 CCGG PleI 643 GACTC GAGTC(4/5) 346 GAGTC Sau96I 723 GGCCC G^G[ACGT]CC 828 GGTCC SphI 80 GCATGC GCATG^C 927 GCATGC TaqI 344 TCGA T^CGA 918 TCGA Tru9I 161 TTAA T^TAA 728 TTAA TthHB8I 344 TCGA T^CGA 918 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AlwI BbvI BcnI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI BstYI Cac8I CfoI DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI Ksp632I MboI MboII MflI MnlI MvaI MwoI NciI NdeII NlaIII NlaIV NspI Sau3AI ScrFI SfaNI SfcI TfiI TseI Tsp509I TspRI XhoII