Restriction map for
pFN21AE6002
(Linear) (Six-base) MAPSORT of: KIEE6002.seq Check: 1898 from: 1 to: 942
KIEE6002.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AccI GT'mk_AC
Cuts at: 0 887 942
Size: 887 55
AfeI AGC'GCT
Cuts at: 0 172 942
Size: 172 770
AleI CACnn'nnGTG
Cuts at: 0 749 942
Size: 749 193
AvaI C'yCGr_G
Cuts at: 0 132 343 942
Size: 132 211 599
Fragments arranged by size:
599 211 132
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 175 208 942
Size: 175 33 734
Fragments arranged by size:
734 175 33
BamHI G'GATC_C
Cuts at: 0 10 942
Size: 10 932
BanI G'GyrC_C
Cuts at: 0 816 942
Size: 816 126
BbsI GAAGACnn'nnnn_
Cuts at: 0 904 942
Size: 904 38
BglI GCCn_nnn'nGGC
Cuts at: 0 706 942
Size: 706 236
BlpI GC'TnA_GC
Cuts at: 0 311 942
Size: 311 631
BmrI ACTGGGnnnn_n'
Cuts at: 0 523 942
Size: 523 419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 687 942
Size: 94 593 255
Fragments arranged by size:
593 255 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 235 459 942
Size: 235 224 483
Fragments arranged by size:
483 235 224
BsaBI GATnn'nnATC
Cuts at: 0 97 942
Size: 97 845
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 319 349 942
Size: 319 30 593
Fragments arranged by size:
593 319 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 942
Size: 126 816
BsiHKAI G_wGCw'C
Cuts at: 0 282 942
Size: 282 660
BsmBI CGTCTCn'nnnn_
Cuts at: 0 630 942
Size: 630 312
Bsp1286I G_dGCh'C
Cuts at: 0 282 942
Size: 282 660
BspHI T'CATG_A
Cuts at: 0 180 942
Size: 180 762
BssSI C'ACGA_G
Cuts at: 0 281 942
Size: 281 661
BstXI CCAn_nnnn'nTGG
Cuts at: 0 805 942
Size: 805 137
BstYI r'GATC_y
Cuts at: 0 10 92 908 942
Size: 10 82 816 34
Fragments arranged by size:
816 82 34 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 681 784 942
Size: 681 103 158
Fragments arranged by size:
681 158 103
BtsI GCAGTG_nn'
Cuts at: 0 327 646 942
Size: 327 319 296
Fragments arranged by size:
327 319 296
EarI CTCTTCn'nnn_
Cuts at: 0 202 664 769 942
Size: 202 462 105 173
Fragments arranged by size:
462 202 173 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 687 942
Size: 94 593 255
Fragments arranged by size:
593 255 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 829 942
Size: 829 113
EcoO109I rG'GnC_Cy
Cuts at: 0 723 942
Size: 723 219
FspI TGC'GCA
Cuts at: 0 682 942
Size: 682 260
HaeII r_GCGC'y
Cuts at: 0 174 942
Size: 174 768
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 900 932 942
Size: 900 32 10
Fragments arranged by size:
900 32 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 942
Size: 255 687
MslI CAynn'nnrTG
Cuts at: 0 749 775 942
Size: 749 26 167
Fragments arranged by size:
749 167 26
NspI r_CATG'y
Cuts at: 0 84 615 931 942
Size: 84 531 316 11
Fragments arranged by size:
531 316 84 11
PasI CC'CwG_GG
Cuts at: 0 6 15 67 942
Size: 6 9 52 875
Fragments arranged by size:
875 52 9 6
PfoI T'CCnGG_A
Cuts at: 0 911 942
Size: 911 31
PshAI GACnn'nnGTC
Cuts at: 0 227 942
Size: 227 715
PspXI vC'TCGA_Gb
Cuts at: 0 343 942
Size: 343 599
SapI GCTCTTCn'nnn_
Cuts at: 0 769 942
Size: 769 173
SexAI A'CCwGG_T
Cuts at: 0 824 942
Size: 824 118
SfcI C'TryA_G
Cuts at: 0 251 629 888 942
Size: 251 378 259 54
Fragments arranged by size:
378 259 251 54
SmaI CCC'GGG
Cuts at: 0 134 942
Size: 134 808
SmlI C'TyrA_G
Cuts at: 0 343 942
Size: 343 599
SphI G_CATG'C
Cuts at: 0 84 931 942
Size: 84 847 11
Fragments arranged by size:
847 84 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 319 351 397 429 638 670 942
Size: 319 32 46 32 209 32 272
Fragments arranged by size:
319 272 209 46 32 32 32
XhoI C'TCGA_G
Cuts at: 0 343 942
Size: 343 599
XmaI C'CCGG_G
Cuts at: 0 132 942
Size: 132 810
Enzymes that do cut:
AccI AfeI AleI AvaI BaeI BamHI BanI BbsI
BglI BlpI BmrI BpmI BsaI BsaBI BsaXI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BssSI BstXI BstYI BtgZI
BtsI EarI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
MmeI MslI NspI PasI PfoI PshAI PspXI SapI
SexAI SfcI SmaI SmlI SphI TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglII Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsiEI BsiWI
BsmI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI StyI SwaI TaqII TatI Tth111I
XbaI XcmI XmnI ZraI