################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwNI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HapII HgaI Hin1I HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 124 GTCTAC GT^[AC][GT]AC Alw44I 89 GTGCAC G^TGCAC Aor51HI 423 AGCGCT AGC^GCT ApaLI 89 GTGCAC G^TGCAC BanI 151 GGCACC G^G[CT][GA]CC BbvCI 305 CCTCAGC CCTCAGC(-5/-2) BglI 677 GCCTGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 484 GGTCTC GGTCTC(1/5) BsmAI 485 GTCTC GTCTC(1/5) BsmFI 298 GTCCC GGGAC(10/14) CfoI 424 GCGC GCG^C DraII 539 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 638 CCATGG C^C[GA][CT]GG Eco47III 423 AGCGCT AGC^GCT EcoNI 531 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 539 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 638 CCATGG C^C[AT][AT]GG FauI 181 CCCGC CCCGC(4/6) HaeII 423 AGCGCT [GA]GCGC^[CT] HhaI 424 GCGC GCG^C HinP1I 424 GCGC G^CGC HincII 3 GTCAAC GT[CT]^[GA]AC HindII 3 GTCAAC GT[CT]^[GA]AC HindIII 596 AAGCTT A^AGCTT HphI 431 TCACC GGTGA(8/7) MaeIII 244 GTCAC ^GT[ACGT]AC MseI 803 TTAA T^TAA NcoI 638 CCATGG C^CATGG PflMI 155 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 539 AGGTCCT [GA]G^G[AT]CC[CT] SphI 71 GCATGC GCATG^C StuI 78 AGGCCT AGG^CCT StyI 638 CCATGG C^C[AT][AT]GG TaqI 474 TCGA T^CGA Tru9I 803 TTAA T^TAA Tsp45I 244 GTCAC ^GT[GC]AC Tsp509I 444 AATT ^AATT TthHB8I 474 TCGA T^CGA Van91I 155 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 53 CCACCTTCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 182 CCGC CCGC(-3/-1) 192 CCGC AluI 597 AGCT AG^CT 815 AGCT AvaII 540 GGTCC G^G[AT]CC 648 GGACC BalI 395 TGGCCA TGG^CCA 461 TGGCCA BseRI 427 CTCCTC GAGGAG(10/8) 766 CTCCTC BsrI 141 ACTGG ACTGG(1/-1) 393 ACTGG EaeI 395 TGGCCA [CT]^GGCC[GA] 461 TGGCCA HinfI 355 GAGTC G^A[ACGT]TC 706 GAGTC MluNI 395 TGGCCA TGG^CCA 461 TGGCCA MscI 395 TGGCCA TGG^CCA 461 TGGCCA MslI 39 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 565 CATCCAGATG NspI 71 GCATGC [GA]CATG^[CT] 518 ACATGC PleI 355 GAGTC GAGTC(4/5) 706 GAGTC PstI 175 CTGCAG CTGCA^G 758 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AlwI AspHI BbvI BmyI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BsrDI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII ItaI Ksp632I MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI