################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbvCI BcgI BclI BcnI BglI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Bsu36I Cac8I CfoI Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI HphI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 841 GTATAC GT^[AC][GT]AC AciI 903 CCGC CCGC(-3/-1) AcsI 28 AAATTT [GA]^AATT[CT] AflII 493 CTTAAG C^TTAAG AflIII 454 ACACGT A^C[GA][CT]GT AluI 292 AGCT AG^CT ApoI 28 AAATTT [GA]^AATT[CT] AseI 603 ATTAAT AT^TAAT AsnI 603 ATTAAT AT^TAAT AspHI 862 GTGCTC G[AT]GC[AT]^C BalI 377 TGGCCA TGG^CCA BciVI 254 GTATCC GTATCC(6/5) BfaI 64 CTAG C^TAG BfrI 493 CTTAAG C^TTAAG BlpI 771 GCTGAGC GC^T[ACGT]AGC BmyI 862 GTGCTC G[AGT]GC[ACT]^C BpmI 341 CTGGAG CTGGAG(16/14) Bpu1102I 771 GCTGAGC GC^T[ACGT]AGC BsaJI 782 CCTTGG C^C[ACGT][ACGT]GG BsiHKAI 862 GTGCTC G[AT]GC[AT]^C Bsp1286I 862 GTGCTC G[AGT]GC[ACT]^C BspMI 845 ACCTGC ACCTGC(4/8) BsrDI 114 CATTGC GCAATG(2/0) BsrFI 49 ACCGGC [GA]^CCGG[CT] BsrI 625 ACTGG ACTGG(1/-1) BssKI 58 CCTGG ^CC[ACGT]GG Bst1107I 841 GTATAC GTA^TAC BstNI 58 CCTGG CC^[AT]GG BstXI 209 CCACTGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 83 GGATCT [GA]^GATC[CT] BstZ17I 841 GTATAC GTA^TAC CelII 771 GCTGAGC GC^T[ACGT]AGC Cfr10I 49 ACCGGC [GA]^CCGG[CT] EaeI 377 TGGCCA [CT]^GGCC[GA] EarI 193 CTCTTC CTCTTC(1/4) EcoRII 58 CCTGG ^CC[AT]GG EcoT14I 782 CCTTGG C^C[AT][AT]GG EcoT22I 642 ATGCAT ATGCA^T HapII 50 CCGG C^CGG HpaII 50 CCGG C^CGG Ksp632I 193 CTCTTC CTCTTC(1/4) MaeI 64 CTAG C^TAG MflI 83 GGATCT [GA]^GATC[CT] MluNI 377 TGGCCA TGG^CCA MscI 377 TGGCCA TGG^CCA MspI 50 CCGG C^CGG MvaI 58 CCTGG CC^[AT]GG NsiI 642 ATGCAT ATGCA^T PshBI 603 ATTAAT AT^TAAT ScaI 465 AGTACT AGT^ACT ScrFI 58 CCTGG CC^[ACGT]GG SfaNI 593 GCATC GCATC(5/9) SmlI 493 CTTAAG C^T[CT][GA]AG SspI 471 AATATT AAT^ATT StyI 782 CCTTGG C^C[AT][AT]GG TaqI 878 TCGA T^CGA Tsp45I 386 GTGAC ^GT[GC]AC TthHB8I 878 TCGA T^CGA XhoII 83 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 399 GTAC GT^AC 466 GTAC AlwI 138 GATCC GGATC(4/5) 83 GGATC AlwNI 206 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 720 CAGCACCTG BbrPI 272 CACGTG CAC^GTG 455 CACGTG BbsI 440 GTCTTC GAAGAC(2/6) 715 GTCTTC BbvI 293 GCTGC GCAGC(8/12) 924 GCTGC BpuAI 440 GTCTTC GAAGAC(2/6) 715 GTCTTC BsaAI 272 CACGTG [CT]AC^GT[GA] 455 CACGTG BsmAI 167 GTCTC GTCTC(1/5) 730 GTCTC BsmI 519 GAATGC GAATGC(1/-1) 53 GCATTC Fnu4HI 293 GCTGC GC^[ACGT]GC 924 GCTGC HaeIII 61 GGCC GG^CC 378 GGCC HincII 45 GTTGAC GT[CT]^[GA]AC 240 GTCAAC HindII 45 GTTGAC GT[CT]^[GA]AC 240 GTCAAC ItaI 293 GCTGC GC^[ACGT]GC 924 GCTGC MaeII 273 ACGT A^CGT 456 ACGT MaeIII 386 GTGAC ^GT[ACGT]AC 542 GTTAC NspI 568 GCATGT [GA]CATG^[CT] 767 ACATGC PleI 67 GAGTC GAGTC(4/5) 165 GAGTC PmaCI 272 CACGTG CAC^GTG 455 CACGTG PmlI 272 CACGTG CAC^GTG 455 CACGTG RsaI 399 GTAC GT^AC 466 GTAC TseI 293 GCTGC G^C[AT]GC 924 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### BsiYI BslI BstF5I DdeI DpnII FokI HinfI MboI MboII MnlI MseI MslI MwoI NdeII NlaIII Sau3AI SfcI TfiI Tru9I Tsp509I TspRI