(Circular) (Six-base) MAPSORT of: pFN21AE5964.seq Check: 4117 from: 1 to: 5641 pFN21AE5964 5641 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2847 2984 1937 Size: 910 137 4594 Fragments arranged by size: 4594 910 137 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2185 2707 2724 3704 5088 5356 1632 Size: 553 522 17 980 1384 268 1917 Fragments arranged by size: 1917 1384 980 553 522 268 17 AflII C'TTAA_G Cuts at: 820 1017 2498 820 Size: 197 1481 3963 Fragments arranged by size: 3963 1481 197 AflIII A'CryG_T Cuts at: 1203 2459 4540 5517 1203 Size: 1256 2081 977 1327 Fragments arranged by size: 2081 1327 1256 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2933 1184 Size: 1749 3892 AlwNI CAG_nnn'CTG Cuts at: 2216 2730 3384 4956 2216 Size: 514 654 1572 2901 Fragments arranged by size: 2901 1572 654 514 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2033 2950 3176 3328 5437 1493 Size: 540 917 226 152 2109 1697 Fragments arranged by size: 2109 1697 917 540 226 152 AseI AT'TA_AT Cuts at: 160 1032 2609 4195 160 Size: 872 1577 1586 1606 Fragments arranged by size: 1606 1586 1577 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2966 1870 Size: 73 1023 4545 Fragments arranged by size: 4545 1023 73 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2962 4417 618 Size: 325 217 627 1175 1455 1842 Fragments arranged by size: 1842 1455 1175 627 325 217 BanII G_rGCy'C Cuts at: 1391 2960 1391 Size: 1569 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2438 2713 928 Size: 971 539 275 3856 Fragments arranged by size: 3856 971 539 275 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2270 3533 4743 2270 Size: 1263 1210 3168 Fragments arranged by size: 3168 1263 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BfrBI ATG'CAT Cuts at: 2649 2649 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4252 136 Size: 107 122 71 631 605 2580 1525 Fragments arranged by size: 2580 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 2777 3058 2777 Size: 281 5360 Bme1580I G_kGCm'C Cuts at: 1790 3702 4858 1790 Size: 1912 1156 2573 Fragments arranged by size: 2573 1912 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2367 4301 1239 Size: 1128 1934 2579 Fragments arranged by size: 2579 1934 1128 Bpu10I CC'TnA_GC Cuts at: 2015 2015 Size: 5641 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2279 2462 3432 493 Size: 711 1075 183 970 2702 Fragments arranged by size: 2702 1075 970 711 183 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3448 3830 4465 275 Size: 53 83 186 936 1915 382 635 1451 Fragments arranged by size: 1915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2340 2370 2478 2508 1178 Size: 30 165 30 937 30 108 30 4311 Fragments arranged by size: 4311 937 165 108 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5641 BseYI C'CCAG_C Cuts at: 2163 4844 5340 2163 Size: 2681 496 2464 Fragments arranged by size: 2681 2464 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5068 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3852 4880 1540 Size: 275 186 1090 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2871 3702 3787 4858 2871 Size: 831 85 1071 3654 Fragments arranged by size: 3654 1071 831 85 BsmI GAATG_Cn' Cuts at: 2058 2530 3152 3245 2058 Size: 472 622 93 4454 Fragments arranged by size: 4454 622 472 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2871 2960 3702 3787 4858 1391 Size: 399 1081 89 742 85 1071 2174 Fragments arranged by size: 2174 1081 1071 742 399 89 85 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2859 2996 844 Size: 2015 137 3489 Fragments arranged by size: 3489 2015 137 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2118 4136 4310 65 Size: 1183 870 2018 174 1396 Fragments arranged by size: 2018 1396 1183 870 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2054 4285 4472 1700 Size: 110 244 2231 187 2869 Fragments arranged by size: 2869 2231 244 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstXI CCAn_nnnn'nTGG Cuts at: 2221 2221 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2088 2971 3338 3723 3740 5192 5278 5290 Size: 116 883 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 883 385 367 346 116 86 17 12 BstZ17I GTA'TAC Cuts at: 2848 2848 Size: 5641 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4150 Fragments arranged by size: 4150 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 3157 3951 3971 3157 Size: 794 20 4827 Fragments arranged by size: 4827 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2472 2507 529 Size: 35 1908 35 3663 Fragments arranged by size: 3663 1908 35 35 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 3088 3977 Size: 54 1425 50 275 18 552 706 889 Cuts at: 3977 5606 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 706 552 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2204 3571 1353 Size: 851 1367 3423 Fragments arranged by size: 3423 1367 851 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2185 2367 2707 2724 3704 4301 5088 Size: 393 553 182 340 17 980 597 787 Cuts at: 5088 5356 1239 Size: 268 1524 Fragments arranged by size: 1524 980 787 597 553 393 340 268 182 17 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 4788 1678 Size: 3110 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2052 2247 2985 3231 669 Size: 988 281 114 195 738 246 3079 Fragments arranged by size: 3079 988 738 281 246 195 114 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 10 Size: 54 1425 343 552 3267 Fragments arranged by size: 3267 1425 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2336 2438 2556 2771 3599 3958 4117 Size: 648 1170 102 118 215 828 359 159 Cuts at: 4117 518 Size: 2042 Fragments arranged by size: 2042 1170 828 648 359 215 159 118 102 MspA1I CmG'CkG Cuts at: 1606 3057 3734 4882 5127 1606 Size: 1451 677 1148 245 2120 Fragments arranged by size: 2120 1451 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4150 Fragments arranged by size: 4150 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NsiI A_TGCA'T Cuts at: 2651 2651 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2577 2776 2999 4544 1132 Size: 97 1348 199 223 1545 2229 Fragments arranged by size: 2229 1545 1348 223 199 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PmlI CAC'GTG Cuts at: 2279 2462 2279 Size: 183 5458 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PstI C_TGCA'G Cuts at: 830 2993 830 Size: 2163 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 2472 3889 5601 2472 Size: 1417 1712 2512 Fragments arranged by size: 2512 1712 1417 SfcI C'TryA_G Cuts at: 826 1045 2361 2466 2515 2989 4124 4805 4996 Size: 219 1316 105 49 474 1135 681 191 Cuts at: 4996 826 Size: 1471 Fragments arranged by size: 1471 1316 1135 681 474 219 191 105 49 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2498 3745 4646 4908 820 Size: 197 926 555 1247 901 262 1553 Fragments arranged by size: 1553 1247 926 901 555 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 2478 3565 5 Size: 47 2426 1087 2081 Fragments arranged by size: 2426 2081 1087 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 2004 2787 3080 513 Size: 552 939 783 293 3074 Fragments arranged by size: 3074 939 783 552 293 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2796 3668 3685 3838 4023 4468 325 Size: 1255 1216 872 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1216 872 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2470 3887 5599 Size: 274 80 33 51 1443 493 1417 1712 Cuts at: 5599 96 Size: 138 Fragments arranged by size: 1712 1443 1417 493 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2340 2372 5601 5633 2340 Size: 32 3229 32 2348 Fragments arranged by size: 3229 2348 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI