Restriction map for
pFN21AE6052
(Circular) (Six-base) MAPSORT of: pFN21AE6052.seq Check: 3460 from: 1 to: 5659
pFN21AE6052 5659 bp
With 182 enzymes: SgfI *
December 8, 2014 13:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 3722 5106 5374 1632
Size: 1302 788 1384 268 1917
Fragments arranged by size:
1917 1384 1302 788 268
AflII C'TTAA_G
Cuts at: 820 1017 2678 820
Size: 197 1661 3801
Fragments arranged by size:
3801 1661 197
AflIII A'CryG_T
Cuts at: 1203 2699 4558 5535 1203
Size: 1496 1859 977 1327
Fragments arranged by size:
1859 1496 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 3402 4974 3402
Size: 1572 4087
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2968 3194 3346 5455 1493
Size: 1475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
BamHI G'GATC_C
Cuts at: 2014 2989 3356 2014
Size: 975 367 4317
Fragments arranged by size:
4317 975 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2482 2980 4435 618
Size: 325 217 627 695 498 1455 1842
Fragments arranged by size:
1842 1455 695 627 498 325 217
BanII G_rGCy'C
Cuts at: 1391 2187 2958 2978 1391
Size: 796 771 20 4072
Fragments arranged by size:
4072 796 771 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2488 2900 928
Size: 971 589 412 3687
Fragments arranged by size:
3687 971 589 412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4270 136
Size: 107 122 71 631 605 2598 1525
Fragments arranged by size:
2598 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 3720 4876 1790
Size: 1930 1156 2573
Fragments arranged by size:
2573 1930 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4319 1239
Size: 3080 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2702 3450 493
Size: 711 1498 748 2702
Fragments arranged by size:
2702 1498 748 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5434
Fragments arranged by size:
5434 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5659
BseYI C'CCAG_C
Cuts at: 4862 5358 4862
Size: 496 5163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2457 1163
Size: 573 721 4365
Fragments arranged by size:
4365 721 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2111 2172 2871 3720 3805 4876 2111
Size: 61 699 849 85 1071 2894
Fragments arranged by size:
2894 1071 849 699 85 61
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
BsmBI CGTCTCn'nnnn_
Cuts at: 2740 2740
Size: 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2172 2187 2871 2958 2978 3720
Size: 399 321 61 15 684 87 20 742
Cuts at: 3720 3805 4876 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 684 399 321 87 85
61 20 15
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 2565 3548 5278 2565
Size: 983 1730 2946
Fragments arranged by size:
2946 1730 983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3014 844
Size: 2170 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2523 3546 1394
Size: 429 700 1023 3507
Fragments arranged by size:
3507 1023 700 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2032 4154 4328 65
Size: 1183 784 2122 174 1396
Fragments arranged by size:
2122 1396 1183 784 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 2430 3719 4731 2430
Size: 1289 1012 3358
Fragments arranged by size:
3358 1289 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2176 794
Size: 1382 4277
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2257 2638 2011
Size: 246 381 5032
Fragments arranged by size:
5032 381 246
BstYI r'GATC_y
Cuts at: 1972 2014 2989 3356 3741 3758 5210 5296 5308
Size: 42 975 367 385 17 1452 86 12
Cuts at: 5308 5654 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 975 385 367 346 86 42
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2364 2787 513
Size: 552 1299 423 3385
Fragments arranged by size:
3385 1299 552 423
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2780 487
Size: 683 56 1554 3366
Fragments arranged by size:
3366 1554 683 56
BtsI GCAGTG_nn'
Cuts at: 2399 3175 3969 3989 2399
Size: 776 794 20 4069
Fragments arranged by size:
4069 794 776 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2361 2385
Size: 54 1425 50 275 18 429 102 24
Cuts at: 2385 2838 2937 3106 3995 5624 8
Size: 453 99 169 889 1629 43
Fragments arranged by size:
1629 1425 889 453 429 275 169 102
99 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 3589 1353
Size: 717 1519 3423
Fragments arranged by size:
3423 1519 717
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2617 4246 4620 4766 387
Size: 1064 138 1028 1629 374 146 1280
Fragments arranged by size:
1629 1280 1064 1028 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2934 3722 4319 5106 5374 1239
Size: 393 1302 788 597 787 268 1524
Fragments arranged by size:
1524 1302 788 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
EcoRV GAT'ATC
Cuts at: 2587 2587
Size: 5659
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2901 2933 4300
Size: 32 135 32 427 32 870 32 1367
Cuts at: 4300 4332 4374 4406 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1367 870 427 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 2877 3258 2877
Size: 381 5278
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2363 2387 2840 2939
Size: 54 1425 343 429 102 24 453 99
Cuts at: 2939 10
Size: 2730
Fragments arranged by size:
2730 1425 453 429 343 102 99 54
24
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2559 2745 3617 3976 4135 518
Size: 648 843 550 186 872 359 159 2042
Fragments arranged by size:
2042 872 843 648 550 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 2509 2824 3075 3752 4900 5145 1606
Size: 903 315 251 677 1148 245 2120
Fragments arranged by size:
2120 1148 903 677 315 251 245
NcoI C'CATG_G
Cuts at: 513 1065 2364 2787 513
Size: 552 1299 423 3385
Fragments arranged by size:
3385 1299 552 423
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2209 3017 4562 1132
Size: 97 980 808 1545 2229
Fragments arranged by size:
2229 1545 980 808 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2268 1108
Size: 1160 4499
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PmlI CAC'GTG
Cuts at: 2702 2702
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2069 2101 3728 3760 5241 5273 2069
Size: 32 1627 32 1481 32 2455
Fragments arranged by size:
2455 1627 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 3011 830
Size: 2181 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2509 2509
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 2418 3907 5619 2418
Size: 1489 1712 2458
Fragments arranged by size:
2458 1712 1489
SfcI C'TryA_G
Cuts at: 826 1045 2309 3007 4142 4823 5014 826
Size: 219 1264 698 1135 681 191 1471
Fragments arranged by size:
1471 1264 1135 698 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 3763 4664 4926 820
Size: 197 926 735 1085 901 262 1553
Fragments arranged by size:
1553 1085 926 901 735 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 2209 3017 1132
Size: 1077 808 3774
Fragments arranged by size:
3774 1077 808
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 2061 1517
Size: 544 5115
StyI C'CwwG_G
Cuts at: 513 1065 2364 2715 2787 3098 513
Size: 552 1299 351 72 311 3074
Fragments arranged by size:
3074 1299 552 351 311 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2412 3686 3703 3856 4041 4486 325
Size: 1255 832 1274 17 153 185 445 1498
Fragments arranged by size:
1498 1274 1255 832 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2416 3905 5617
Size: 274 80 33 51 1443 439 1489 1712
Cuts at: 5617 96
Size: 138
Fragments arranged by size:
1712 1489 1443 439 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2333 2356 2446 2333
Size: 23 90 5546
Fragments arranged by size:
5546 90 23
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI