################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AfaI 183 GTAC GT^AC AflIII 724 ACATGT A^C[GA][CT]GT AluI 583 AGCT AG^CT AvaI 455 CCCGGG C^[CT]CG[GA]G BanI 742 GGTGCC G^G[CT][GA]CC BbvCI 230 CCTCAGC CCTCAGC(-5/-2) BfaI 581 CTAG C^TAG BlpI 101 GCTTAGC GC^T[ACGT]AGC Bpu1102I 101 GCTTAGC GC^T[ACGT]AGC BsaBI 84 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 228 CTCCTC GAGGAG(10/8) BsmAI 730 GTCTC GTCTC(1/5) BsoBI 455 CCCGGG C^[CT]CG[GA]G BspLU11I 724 ACATGT A^CATGT BstXI 209 CCATCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 83 GGATCT [GA]^GATC[CT] CelII 101 GCTTAGC GC^T[ACGT]AGC HapII 456 CCGG C^CGG HpaII 456 CCGG C^CGG MaeI 581 CTAG C^TAG MaeIII 463 GTGAC ^GT[ACGT]AC MamI 84 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 83 GGATCT [GA]^GATC[CT] MseI 22 TTAA T^TAA MspI 456 CCGG C^CGG NspI 724 ACATGT [GA]CATG^[CT] PflMI 254 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 723 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 183 GTAC GT^AC SapI 849 GCTCTTC GCTCTTC(1/4) SexAI 215 ACCTGGT A^CC[AT]GGT SmaI 455 CCCGGG CCC^GGG StuI 60 AGGCCT AGG^CCT TaqI 212 TCGA T^CGA TfiI 54 GATTC G^A[AT]TC Tru9I 22 TTAA T^TAA Tsp45I 463 GTGAC ^GT[GC]AC Tsp509I 838 AATT ^AATT TthHB8I 212 TCGA T^CGA Van91I 254 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 83 GGATCT [GA]^GATC[CT] XmaCI 455 CCCGGG C^CCGGG XmaI 455 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 83 GGATC GGATC(4/5) 270 GGATC AspI 214 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 414 GACTGGGTC BcnI 455 CCCGG CC^[GC]GG 456 CCGGG BglI 196 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 292 GCCTGTCTGGC BspMI 71 ACCTGC ACCTGC(4/8) 635 ACCTGC DraII 407 AGGTCCT [GA]G^G[ACGT]CC[CT] 418 GGGTCCC DsaI 356 CCATGG C^C[GA][CT]GG 860 CCATGG EarI 777 CTCTTC CTCTTC(1/4) 850 CTCTTC EcoO109I 407 AGGTCCT [GA]G^G[ACGT]CC[CT] 418 GGGTCCC FauI 704 CCCGC CCCGC(4/6) 800 CCCGC Ksp632I 777 CTCTTC CTCTTC(1/4) 850 CTCTTC NciI 455 CCCGG CC^[GC]GG 456 CCGGG NcoI 356 CCATGG C^CATGG 860 CCATGG PflFI 214 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 414 GACTGGGTC PleI 474 GACTC GAGTC(4/5) 337 GAGTC PpuMI 407 AGGTCCT [GA]G^G[AT]CC[CT] 418 GGGTCCC Tth111I 214 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 414 GACTGGGTC XcmI 431 CCAAGATTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 614 CCATGTTTATTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AvaII BalI BbvI BsaJI BsgI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MboI MboII MluNI MnlI MscI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI