(Circular) (Six-base) MAPSORT of: pFN21AE6005.seq Check: 3026 from: 1 to: 5647 pFN21AE6005 5647 bp With 182 enzymes: SgfI * December 8, 2014 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2085 2085 Size: 5647 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2990 1937 Size: 1053 4594 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2660 2769 3710 5094 5362 1632 Size: 1028 109 941 1384 268 1917 Fragments arranged by size: 1917 1384 1028 941 268 109 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 2729 4546 5523 1203 Size: 1526 1817 977 1327 Fragments arranged by size: 1817 1526 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 2771 1166 Size: 1605 4042 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2414 1184 Size: 1230 4417 AlwNI CAG_nnn'CTG Cuts at: 3390 4962 3390 Size: 1572 4075 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2956 3182 3334 5443 1493 Size: 1463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1463 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2460 2972 1870 Size: 73 517 512 4545 Fragments arranged by size: 4545 517 512 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2171 2204 2171 Size: 33 5614 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2747 2968 4423 618 Size: 325 217 627 960 221 1455 1842 Fragments arranged by size: 1842 1455 960 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2966 1391 Size: 1575 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4676 BbvCI CC'TCA_GC Cuts at: 2236 2236 Size: 5647 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3838 3872 1903 Size: 34 1901 34 3678 Fragments arranged by size: 3678 1901 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 4749 3539 Size: 1210 4437 BclI T'GATC_A Cuts at: 1548 1548 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2207 2303 4258 Size: 107 122 71 631 605 535 96 1955 Cuts at: 4258 136 Size: 1525 Fragments arranged by size: 1955 1525 631 605 535 122 107 96 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 2107 3064 2107 Size: 957 4690 Bme1580I G_kGCm'C Cuts at: 1790 3708 4864 1790 Size: 1918 1156 2573 Fragments arranged by size: 2573 1918 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2430 2519 3378 Size: 416 53 83 399 24 1013 89 859 Cuts at: 3378 4336 442 Size: 958 1753 Fragments arranged by size: 1753 1013 958 859 416 399 89 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4307 1239 Size: 3068 2579 Bpu10I CC'TnA_GC Cuts at: 2236 2236 Size: 5647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3772 4637 4935 3772 Size: 865 298 4484 Fragments arranged by size: 4484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3438 493 Size: 711 2234 2702 Fragments arranged by size: 2702 2234 711 BsaBI GATnn'nnATC Cuts at: 2093 5641 2093 Size: 3548 2099 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3454 3836 4471 275 Size: 53 83 186 936 1921 382 635 1451 Fragments arranged by size: 1921 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 1178 Size: 30 165 30 912 30 4480 Fragments arranged by size: 4480 912 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2224 1186 Size: 1038 4609 BseYI C'CCAG_C Cuts at: 4850 5346 4850 Size: 496 5151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 2122 2384 1163 Size: 573 327 59 262 4426 Fragments arranged by size: 4426 573 327 262 59 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3708 3793 4864 3708 Size: 85 1071 4491 Fragments arranged by size: 4491 1071 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2966 3708 3793 4864 1391 Size: 399 1176 742 85 1071 2174 Fragments arranged by size: 2174 1176 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2085 2649 3002 844 Size: 1241 564 353 3489 Fragments arranged by size: 3489 1241 564 353 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4291 4478 1700 Size: 110 2481 187 2869 Fragments arranged by size: 2869 2481 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5647 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5647 BstXI CCAn_nnnn'nTGG Cuts at: 2221 2221 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2088 2977 3344 3729 3746 5198 5284 5296 Size: 116 889 367 385 17 1452 86 12 Cuts at: 5296 5642 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 889 385 367 346 116 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2361 2865 513 Size: 552 1296 504 3295 Fragments arranged by size: 3295 1296 552 504 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2593 487 Size: 683 56 1367 3541 Fragments arranged by size: 3541 1367 683 56 BtsI GCAGTG_nn' Cuts at: 2240 2355 2396 2536 3163 3957 3977 2240 Size: 115 41 140 627 794 20 3910 Fragments arranged by size: 3910 794 627 140 115 41 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2358 2616 Size: 54 1425 50 275 18 369 159 258 Cuts at: 2616 3094 3983 5612 8 Size: 478 889 1629 43 Fragments arranged by size: 1629 1425 889 478 369 275 258 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2788 2861 3577 1353 Size: 1435 73 716 3423 Fragments arranged by size: 3423 1435 716 73 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2660 2769 3710 4307 5094 5362 1239 Size: 393 1028 109 941 597 787 268 1524 Fragments arranged by size: 1524 1028 941 787 597 393 268 109 EcoO109I rG'GnC_Cy Cuts at: 2413 2424 2413 Size: 11 5636 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 4794 1678 Size: 3116 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4288 4320 4362 4394 1373 Size: 32 135 32 2716 32 42 32 2626 Fragments arranged by size: 2716 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2060 2616 4761 4945 1305 Size: 755 556 2145 184 2007 Fragments arranged by size: 2145 2007 755 556 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2360 2618 10 Size: 54 1425 343 369 159 258 3039 Fragments arranged by size: 3039 1425 369 343 258 159 54 MslI CAynn'nnrTG Cuts at: 518 1166 2558 2612 2745 2771 3605 3964 4123 Size: 648 1392 54 133 26 834 359 159 Cuts at: 4123 518 Size: 2042 Fragments arranged by size: 2042 1392 834 648 359 159 133 54 26 MspA1I CmG'CkG Cuts at: 1606 3063 3740 4888 5133 1606 Size: 1457 677 1148 245 2120 Fragments arranged by size: 2120 1457 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2361 2865 513 Size: 552 1296 504 3295 Fragments arranged by size: 3295 1296 552 504 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 2733 3005 4550 1132 Size: 97 1504 272 1545 2229 Fragments arranged by size: 2229 1545 1504 272 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 2729 4546 2729 Size: 1817 3830 PflMI CCAn_nnn'nTGG Cuts at: 1108 2265 1108 Size: 1157 4490 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2413 2424 2413 Size: 11 5636 PshAI GACnn'nnGTC Cuts at: 1613 2732 1613 Size: 1119 4528 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PstI C_TGCA'G Cuts at: 830 2999 830 Size: 2169 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SanDI GG'GwC_CC Cuts at: 2424 2424 Size: 5647 SapI GCTCTTCn'nnn_ Cuts at: 2861 2861 Size: 5647 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SexAI A'CCwGG_T Cuts at: 2220 2220 Size: 5647 SfcI C'TryA_G Cuts at: 826 1045 2995 4130 4811 5002 826 Size: 219 1950 1135 681 191 1471 Fragments arranged by size: 1950 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2462 2974 1872 Size: 590 512 4545 Fragments arranged by size: 4545 590 512 SmlI C'TyrA_G Cuts at: 820 1017 1943 3751 4652 4914 820 Size: 197 926 1808 901 262 1553 Fragments arranged by size: 1808 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SrfI GCCC'GGGC Cuts at: 2462 2462 Size: 5647 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 2067 1517 Size: 550 5097 StyI C'CwwG_G Cuts at: 513 1065 2361 2409 2865 3086 513 Size: 552 1296 48 456 221 3074 Fragments arranged by size: 3074 1296 552 456 221 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2878 3674 3691 3844 4029 4474 325 Size: 1255 1298 796 17 153 185 445 1498 Fragments arranged by size: 1498 1298 1255 796 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3893 5605 96 Size: 274 80 33 51 1443 1916 1712 138 Fragments arranged by size: 1916 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2475 2507 5607 5639 2315 Size: 32 128 32 3100 32 2323 Fragments arranged by size: 3100 2323 128 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2222 2422 1316 Size: 906 200 4541 Fragments arranged by size: 4541 906 200 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2443 2626 2443 Size: 183 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2460 2972 1870 Size: 590 512 4545 Fragments arranged by size: 4545 590 512 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI SwaI