(Circular) (Six-base) MAPSORT of: pFN21AE2791.seq Check: 9104 from: 1 to: 8200 pFN21AE2791 8200 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2196 2946 3842 4481 2196 Size: 750 896 639 5915 Fragments arranged by size: 5915 896 750 639 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4623 6010 278 Size: 53 83 186 936 3087 1387 2468 Fragments arranged by size: 3087 2468 1387 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5543 1937 Size: 3606 4594 Acc65I G'GTAC_C Cuts at: 5521 5521 Size: 8200 AclI AA'CG_TT Cuts at: 1563 6327 6700 1563 Size: 4764 373 3063 Fragments arranged by size: 4764 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2070 3017 3988 4258 6263 7647 7915 1632 Size: 438 947 971 270 2005 1384 268 1917 Fragments arranged by size: 2005 1917 1384 971 947 438 270 268 AfeI AGC'GCT Cuts at: 4994 4994 Size: 8200 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8003 AflIII A'CryG_T Cuts at: 1203 4944 4974 7099 8076 1203 Size: 3741 30 2125 977 1327 Fragments arranged by size: 3741 2125 1327 977 30 AgeI A'CCGG_T Cuts at: 7031 7031 Size: 8200 AhdI GACnn_n'nnGTC Cuts at: 6929 6929 Size: 8200 AleI CACnn'nnGTG Cuts at: 1166 4904 5445 1166 Size: 3738 541 3921 Fragments arranged by size: 3921 3738 541 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3299 4499 1184 Size: 2115 1200 4885 Fragments arranged by size: 4885 2115 1200 AlwNI CAG_nnn'CTG Cuts at: 2938 3912 4051 4684 5943 7515 2938 Size: 974 139 633 1259 1572 3623 Fragments arranged by size: 3623 1572 1259 974 633 139 ApaI G_GGCC'C Cuts at: 2519 3494 4295 4305 4586 2519 Size: 975 801 10 281 6133 Fragments arranged by size: 6133 975 801 281 10 ApaLI G'TGCA_C Cuts at: 6257 7413 6257 Size: 1156 7044 ApoI r'AATT_y Cuts at: 1493 4181 5509 5735 5887 7996 1493 Size: 2688 1328 226 152 2109 1697 Fragments arranged by size: 2688 2109 1697 1328 226 152 AseI AT'TA_AT Cuts at: 160 1032 6754 160 Size: 872 5722 1606 Fragments arranged by size: 5722 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8200 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2575 2770 3182 3357 3708 4304 Size: 73 96 536 195 412 175 351 596 Cuts at: 4304 5279 5471 5525 1870 Size: 975 192 54 4545 Fragments arranged by size: 4545 975 596 536 412 351 195 192 175 96 73 54 BamHI G'GATC_C Cuts at: 2310 5530 5897 2310 Size: 3220 367 4613 Fragments arranged by size: 4613 3220 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2131 2184 2824 3344 4120 Size: 325 217 627 344 53 640 520 776 Cuts at: 4120 5521 6976 618 Size: 1401 1455 1842 Fragments arranged by size: 1842 1455 1401 776 640 627 520 344 325 217 53 BanII G_rGCy'C Cuts at: 1391 2519 2972 3060 3258 3494 4079 4132 4295 Size: 1128 453 88 198 236 585 53 163 Cuts at: 4295 4305 4586 5399 5519 1391 Size: 10 281 813 120 4072 Fragments arranged by size: 4072 1128 813 585 453 281 236 198 163 120 88 53 10 BbeI G_GCGC'C Cuts at: 4124 4124 Size: 8200 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 4150 4615 5077 928 Size: 971 159 2092 465 462 4051 Fragments arranged by size: 4051 2092 971 465 462 159 BbvCI CC'TCA_GC Cuts at: 2609 2609 Size: 8200 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2774 2808 4498 4532 6391 Size: 34 50 34 753 34 1690 34 1859 Cuts at: 6391 6425 1903 Size: 34 3678 Fragments arranged by size: 3678 1859 1690 753 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6092 7302 6092 Size: 1210 6990 BclI T'GATC_A Cuts at: 1548 1548 Size: 8200 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3650 3704 3854 Size: 107 122 71 631 605 1978 54 150 Cuts at: 3854 6811 136 Size: 2957 1525 Fragments arranged by size: 2957 1978 1525 631 605 150 122 107 71 54 BglII A'GATC_T Cuts at: 3105 4752 8195 3105 Size: 1647 3443 3110 Fragments arranged by size: 3443 3110 1647 BlpI GC'TnA_GC Cuts at: 5054 5100 5617 5054 Size: 46 517 7637 Fragments arranged by size: 7637 517 46 Bme1580I G_kGCm'C Cuts at: 1790 2326 2519 2657 2829 3347 3494 4295 4305 Size: 536 193 138 172 518 147 801 10 Cuts at: 4305 4586 6261 7417 1790 Size: 281 1675 1156 2573 Fragments arranged by size: 2573 1675 1156 801 536 518 281 193 172 147 138 10 BmgBI CAC'GTC Cuts at: 1318 2388 3121 3220 5368 1318 Size: 1070 733 99 2148 4150 Fragments arranged by size: 4150 2148 1070 733 99 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5931 6889 442 Size: 416 53 83 399 24 4514 958 1753 Fragments arranged by size: 4514 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8200 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2740 3983 2740 Size: 1243 6957 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2477 2636 2710 3164 3707 3841 4055 4364 Size: 1238 159 74 454 543 134 214 309 Cuts at: 4364 4730 4844 5141 5339 6860 1239 Size: 366 114 297 198 1521 2579 Fragments arranged by size: 2579 1521 1238 543 454 366 309 297 214 198 159 134 114 74 Bpu10I CC'TnA_GC Cuts at: 2609 4131 5063 5192 2609 Size: 1522 932 129 5617 Fragments arranged by size: 5617 1522 932 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3044 3398 6325 7190 7488 3044 Size: 354 2927 865 298 3756 Fragments arranged by size: 3756 2927 865 354 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3406 5121 6863 882 Size: 617 1907 1715 1742 2219 Fragments arranged by size: 2219 1907 1742 1715 617 BsaAI yAC'GTr Cuts at: 493 1204 4151 5991 493 Size: 711 2947 1840 2702 Fragments arranged by size: 2947 2702 1840 711 BsaBI GATnn'nnATC Cuts at: 4556 8194 4556 Size: 3638 4562 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3162 3432 3861 4121 Size: 53 83 186 936 1629 270 429 260 Cuts at: 4121 4620 6007 6389 7024 275 Size: 499 1387 382 635 1451 Fragments arranged by size: 1629 1451 1387 936 635 499 429 382 270 260 186 83 53 BsaWI w'CCGG_w Cuts at: 6633 7031 7305 7452 7863 6633 Size: 398 274 147 411 6970 Fragments arranged by size: 6970 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3116 3146 3386 3416 3740 Size: 30 165 30 1713 30 240 30 324 Cuts at: 3740 3770 1178 Size: 30 5608 Fragments arranged by size: 5608 1713 324 240 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2474 2990 3161 3329 4121 5227 1186 Size: 1054 234 516 171 168 792 1106 4159 Fragments arranged by size: 4159 1106 1054 792 516 234 171 168 BseYI C'CCAG_C Cuts at: 2065 2428 2864 3348 4579 4844 5460 7403 7899 Size: 363 436 484 1231 265 616 1943 496 Cuts at: 7899 2065 Size: 2366 Fragments arranged by size: 2366 1943 1231 616 496 484 436 363 265 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2426 3377 3824 4673 4979 1163 Size: 573 690 951 447 849 306 4384 Fragments arranged by size: 4384 951 849 690 573 447 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3460 4705 5650 6411 7439 1540 Size: 275 186 1459 1245 945 761 1028 2301 Fragments arranged by size: 2301 1459 1245 1028 945 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2160 2300 3060 3258 4734 5106 5118 5349 5399 Size: 140 760 198 1476 372 12 231 50 Cuts at: 5399 6261 6346 7417 2160 Size: 862 85 1071 2943 Fragments arranged by size: 2943 1476 1071 862 760 372 231 198 140 85 50 12 BsmI GAATG_Cn' Cuts at: 2451 5711 5804 2451 Size: 3260 93 4847 Fragments arranged by size: 4847 3260 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3683 3683 Size: 8200 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2300 2326 2519 2657 2829 2972 Size: 399 370 140 26 193 138 172 143 Cuts at: 2972 3060 3258 3347 3494 4079 4132 4295 4305 Size: 88 198 89 147 585 53 163 10 Cuts at: 4305 4586 4734 5106 5118 5349 5399 5519 6261 Size: 281 148 372 12 231 50 120 742 Cuts at: 6261 6346 7417 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 585 399 372 370 281 231 198 193 172 163 148 147 143 140 138 120 89 88 85 53 50 26 12 10 BspEI T'CCGG_A Cuts at: 7863 7863 Size: 8200 BspHI T'CATG_A Cuts at: 4914 6089 7819 4914 Size: 1175 1730 5295 Fragments arranged by size: 5295 1730 1175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 2196 2946 3303 3842 4050 4481 4755 Size: 1331 21 750 357 539 208 431 274 Cuts at: 4755 4929 5139 5555 844 Size: 174 210 416 3489 Fragments arranged by size: 3489 1331 750 539 431 416 357 274 210 208 174 21 BsrBI CCG'CTC Cuts at: 1394 1823 2139 3264 3736 6087 1394 Size: 429 316 1125 472 2351 3507 Fragments arranged by size: 3507 2351 1125 472 429 316 BsrDI GCAATG_nn' Cuts at: 65 1248 5265 6695 6869 65 Size: 1183 4017 1430 174 1396 Fragments arranged by size: 4017 1430 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2055 2191 3335 3379 3459 4737 6844 Size: 110 245 136 1144 44 80 1278 2107 Cuts at: 6844 7031 1700 Size: 187 2869 Fragments arranged by size: 2869 2107 1278 1144 245 187 136 110 80 44 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8200 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8200 BssSI C'ACGA_G Cuts at: 3395 3596 4655 5018 5348 6260 7272 3395 Size: 201 1059 363 330 912 1012 4323 Fragments arranged by size: 4323 1059 1012 912 363 330 201 BstAPI GCAn_nnn'nTGC Cuts at: 794 2362 794 Size: 1568 6632 BstBI TT'CG_AA Cuts at: 7004 7004 Size: 8200 BstEII G'GTnAC_C Cuts at: 1184 4136 4309 4882 4897 1184 Size: 2952 173 573 15 4487 Fragments arranged by size: 4487 2952 573 173 15 BstXI CCAn_nnnn'nTGG Cuts at: 2206 2713 2734 4864 5447 2206 Size: 507 21 2130 583 4959 Fragments arranged by size: 4959 2130 583 507 21 BstYI r'GATC_y Cuts at: 1972 2310 2670 2721 3105 3909 4716 4752 4764 Size: 338 360 51 384 804 807 36 12 Cuts at: 4764 5530 5897 6282 6299 7751 7837 7849 8195 Size: 766 367 385 17 1452 86 12 346 Cuts at: 8195 1972 Size: 1977 Fragments arranged by size: 1977 1452 807 804 766 385 384 367 360 346 338 86 51 36 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2493 3039 4022 4297 2493 Size: 546 983 275 6396 Fragments arranged by size: 6396 983 546 275 BtgI C'CryG_G Cuts at: 513 1065 2733 2750 3069 3369 3507 3621 4521 Size: 552 1668 17 319 300 138 114 900 Cuts at: 4521 5358 513 Size: 837 3355 Fragments arranged by size: 3355 1668 900 837 552 319 300 138 114 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2601 2764 3076 4912 487 Size: 683 56 1375 163 312 1836 3775 Fragments arranged by size: 3775 1836 1375 683 312 163 56 BtsI GCAGTG_nn' Cuts at: 4814 5716 6510 6530 4814 Size: 902 794 20 6484 Fragments arranged by size: 6484 902 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8200 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8165 DraI TTT'AAA Cuts at: 5504 5504 Size: 8200 DraIII CAC_nnn'GTG Cuts at: 4390 5257 4390 Size: 867 7333 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3167 5153 7207 809 Size: 507 1851 1986 2054 1802 Fragments arranged by size: 2054 1986 1851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2340 2379 2835 Size: 54 1425 50 275 18 510 39 456 Cuts at: 2835 3066 3222 3333 3457 3461 3495 3603 3651 Size: 231 156 111 124 4 34 108 48 Cuts at: 3651 3846 3927 4443 4504 4702 4759 5479 5647 Size: 195 81 516 61 198 57 720 168 Cuts at: 5647 6536 8165 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 720 516 510 456 275 231 198 195 168 156 124 111 108 81 61 57 54 50 48 43 39 34 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3457 4702 5647 1537 Size: 275 1645 1245 945 4090 Fragments arranged by size: 4090 1645 1245 945 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3293 3536 3758 4067 4247 4640 6130 1353 Size: 1940 243 222 309 180 393 1490 3423 Fragments arranged by size: 3423 1940 1490 393 309 243 222 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3497 4192 4712 5066 6787 7161 Size: 1064 138 1908 695 520 354 1721 374 Cuts at: 7161 7307 387 Size: 146 1280 Fragments arranged by size: 1908 1721 1280 1064 695 520 374 354 146 138 EcoICRI GAG'CTC Cuts at: 3058 3256 5397 3058 Size: 198 2141 5861 Fragments arranged by size: 5861 2141 198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2070 2477 2636 2710 3017 3164 3707 Size: 393 438 407 159 74 307 147 543 Cuts at: 3707 3841 3988 4055 4258 4364 4730 4844 5141 Size: 134 147 67 203 106 366 114 297 Cuts at: 5141 5339 6263 6860 7647 7915 1239 Size: 198 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 597 543 438 407 393 366 307 297 268 203 198 159 147 147 134 114 106 74 67 EcoNI CCTnn'n_nnAGG Cuts at: 2716 3146 3395 2716 Size: 430 249 7521 Fragments arranged by size: 7521 430 249 EcoO109I rG'GnC_Cy Cuts at: 2021 2515 2516 2570 3490 3562 4039 4048 4291 Size: 494 1 54 920 72 477 9 243 Cuts at: 4291 4292 4301 4583 2021 Size: 1 9 282 5638 Fragments arranged by size: 5638 920 494 477 282 243 72 54 9 9 1 1 EcoRI G'AATT_C Cuts at: 5509 5887 5509 Size: 378 7822 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3889 4639 5203 3889 Size: 750 564 6886 Fragments arranged by size: 6886 750 564 FseI GG_CCGG'CC Cuts at: 3339 3463 3339 Size: 124 8076 FspI TGC'GCA Cuts at: 2270 6706 2270 Size: 4436 3764 HaeII r_GCGC'y Cuts at: 1678 3005 4124 4194 4685 4996 5026 7347 1678 Size: 1327 1119 70 491 311 30 2321 2531 Fragments arranged by size: 2531 2321 1327 1119 491 311 70 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2295 2327 3116 3148 3740 Size: 32 135 32 723 32 789 32 592 Cuts at: 3740 3772 4820 4852 6841 6873 6915 6947 1373 Size: 32 1048 32 1989 32 42 32 2626 Fragments arranged by size: 2626 1989 1048 789 723 592 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2089 5544 5790 669 Size: 988 281 151 3455 246 3079 Fragments arranged by size: 3455 3079 988 281 246 151 HindIII A'AGCT_T Cuts at: 748 748 Size: 8200 HpaI GTT'AAC Cuts at: 5790 5790 Size: 8200 KasI G'GCGC_C Cuts at: 4120 4120 Size: 8200 KpnI G_GTAC'C Cuts at: 5525 5525 Size: 8200 MfeI C'AATT_G Cuts at: 5799 5799 Size: 8200 MluI A'CGCG_T Cuts at: 8076 8076 Size: 8200 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7314 7498 1305 Size: 6009 184 2007 Fragments arranged by size: 6009 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2342 2381 3605 3848 4445 Size: 54 1425 343 510 39 1224 243 597 Cuts at: 4445 5481 10 Size: 1036 2729 Fragments arranged by size: 2729 1425 1224 1036 597 510 343 243 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2558 4695 4904 4931 5445 6158 6517 Size: 648 1392 2137 209 27 514 713 359 Cuts at: 6517 6676 518 Size: 159 2042 Fragments arranged by size: 2137 2042 1392 713 648 514 359 209 159 27 MspA1I CmG'CkG Cuts at: 1606 3304 3371 3816 4081 4579 5425 5616 6293 Size: 1698 67 445 265 498 846 191 677 Cuts at: 6293 7441 7686 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1698 1148 846 677 498 445 265 245 191 67 NaeI GCC'GGC Cuts at: 2193 3337 3461 2193 Size: 1144 124 6932 Fragments arranged by size: 6932 1144 124 NarI GG'CG_CC Cuts at: 4121 4121 Size: 8200 NcoI C'CATG_G Cuts at: 513 1065 3621 4521 513 Size: 552 2556 900 4192 Fragments arranged by size: 4192 2556 900 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8200 NgoMIV G'CCGG_C Cuts at: 2191 3335 3459 2191 Size: 1144 124 6932 Fragments arranged by size: 6932 1144 124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8200 NotI GC'GGCC_GC Cuts at: 5647 5647 Size: 8200 NspI r_CATG'y Cuts at: 1132 1229 2351 4978 5558 7103 1132 Size: 97 1122 2627 580 1545 2229 Fragments arranged by size: 2627 2229 1545 1122 580 97 PasI CC'CwG_GG Cuts at: 1348 4288 5377 1348 Size: 2940 1089 4171 Fragments arranged by size: 4171 2940 1089 PciI A'CATG_T Cuts at: 4974 7099 4974 Size: 2125 6075 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2821 3601 3912 5257 5428 1108 Size: 1269 444 780 311 1345 171 3880 Fragments arranged by size: 3880 1345 1269 780 444 311 171 PfoI T'CCnGG_A Cuts at: 2029 2029 Size: 8200 PmeI GTTT'AAAC Cuts at: 5504 5504 Size: 8200 PmlI CAC'GTG Cuts at: 4151 4151 Size: 8200 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2909 2941 6269 6301 7782 7814 2909 Size: 32 3328 32 1481 32 3295 Fragments arranged by size: 3328 3295 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3562 4039 4048 3562 Size: 477 9 7714 Fragments arranged by size: 7714 477 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8200 PsiI TTA'TAA Cuts at: 5770 5770 Size: 8200 PspOMI G'GGCC_C Cuts at: 2515 3490 4291 4301 4582 2515 Size: 975 801 10 281 6133 Fragments arranged by size: 6133 975 801 281 10 PstI C_TGCA'G Cuts at: 830 2916 3252 3303 3429 3588 4047 4095 4478 Size: 2086 336 51 126 159 459 48 383 Cuts at: 4478 4752 5552 830 Size: 274 800 3478 Fragments arranged by size: 3478 2086 800 459 383 336 274 159 126 51 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8200 PvuII CAG'CTG Cuts at: 4579 5425 4579 Size: 846 7354 SacI G_AGCT'C Cuts at: 3060 3258 5399 3060 Size: 198 2141 5861 Fragments arranged by size: 5861 2141 198 SacII CC_GC'GG Cuts at: 3372 3372 Size: 8200 SalI G'TCGA_C Cuts at: 1936 5542 1936 Size: 3606 4594 SapI GCTCTTCn'nnn_ Cuts at: 3293 3536 4067 3293 Size: 243 531 7426 Fragments arranged by size: 7426 531 243 SbfI CC_TGCA'GG Cuts at: 3429 4047 4478 5552 3429 Size: 618 431 1074 6077 Fragments arranged by size: 6077 1074 618 431 ScaI AGT'ACT Cuts at: 6448 8160 6448 Size: 1712 6488 SexAI A'CCwGG_T Cuts at: 2202 4386 2202 Size: 2184 6016 SfcI C'TryA_G Cuts at: 826 1045 2912 3248 3299 3425 3584 3766 4043 Size: 219 1867 336 51 126 159 182 277 Cuts at: 4043 4091 4474 4748 5548 6683 7364 7555 826 Size: 48 383 274 800 1135 681 191 1471 Fragments arranged by size: 1867 1471 1135 800 681 383 336 277 274 219 191 182 159 126 51 48 SfiI GGCCn_nnn'nGGCC Cuts at: 3704 3704 Size: 8200 SfoI GGC'GCC Cuts at: 4122 4122 Size: 8200 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8200 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8200 SmaI CCC'GGG Cuts at: 1872 2041 2577 3359 5473 5527 1872 Size: 169 536 782 2114 54 4545 Fragments arranged by size: 4545 2114 782 536 169 54 SmlI C'TyrA_G Cuts at: 820 1017 1943 3059 3413 6304 7205 7467 820 Size: 197 926 1116 354 2891 901 262 1553 Fragments arranged by size: 2891 1553 1116 926 901 354 262 197 SnaBI TAC'GTA Cuts at: 493 5991 493 Size: 5498 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8200 SphI G_CATG'C Cuts at: 1132 5558 1132 Size: 4426 3774 SspI AAT'ATT Cuts at: 5 52 6124 5 Size: 47 6072 2081 Fragments arranged by size: 6072 2081 47 StuI AGG'CCT Cuts at: 1517 3869 3896 4032 1517 Size: 2352 27 136 5685 Fragments arranged by size: 5685 2352 136 27 StyI C'CwwG_G Cuts at: 513 1065 2017 2394 2712 2829 3621 3864 4521 Size: 552 952 377 318 117 792 243 657 Cuts at: 4521 5639 513 Size: 1118 3074 Fragments arranged by size: 3074 1118 952 792 657 552 377 318 243 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2210 3362 4155 4454 4691 4823 4827 Size: 1255 630 1152 793 299 237 132 4 Cuts at: 4827 6227 6244 6397 6582 7027 325 Size: 1400 17 153 185 445 1498 Fragments arranged by size: 1498 1400 1255 1152 793 630 445 299 237 185 153 132 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4227 6446 8158 Size: 274 80 33 51 1443 2250 2219 1712 Cuts at: 8158 96 Size: 138 Fragments arranged by size: 2250 2219 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3110 3121 3142 3153 3386 3418 4417 4449 8160 Size: 11 21 11 233 32 999 32 3711 Cuts at: 8160 8192 3110 Size: 32 3118 Fragments arranged by size: 3711 3118 999 233 32 32 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 2987 1316 Size: 1671 6529 XbaI T'CTAG_A Cuts at: 5536 5536 Size: 8200 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 4528 4594 2401 Size: 2127 66 6007 Fragments arranged by size: 6007 2127 66 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8200 XmaI C'CCGG_G Cuts at: 1870 2039 2575 3357 5471 5525 1870 Size: 169 536 782 2114 54 4545 Fragments arranged by size: 4545 2114 782 536 169 54 XmnI GAAnn'nnTTC Cuts at: 4397 6329 4397 Size: 1932 6268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4621 6008 276 Size: 53 83 186 936 3087 1387 2468 Fragments arranged by size: 3087 2468 1387 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SrfI SwaI