(Linear) (Six-base) MAPSORT of: KIEE5178.seq Check: 6796 from: 1 to: 459 KIEE5178.orf With 182 enzymes: SgfI * January 29, 2014 11:49 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 459 Size: 374 85 AfeI AGC'GCT Cuts at: 0 363 459 Size: 363 96 AvaI C'yCGr_G Cuts at: 0 106 169 334 388 459 Size: 106 63 165 54 71 Fragments arranged by size: 165 106 71 63 54 BamHI G'GATC_C Cuts at: 0 128 459 Size: 128 331 BanII G_rGCy'C Cuts at: 0 352 459 Size: 352 107 BglI GCCn_nnn'nGGC Cuts at: 0 356 459 Size: 356 103 BglII A'GATC_T Cuts at: 0 324 459 Size: 324 135 Bme1580I G_kGCm'C Cuts at: 0 191 459 Size: 191 268 BsaAI yAC'GTr Cuts at: 0 288 459 Size: 288 171 BsaHI Gr'CG_yC Cuts at: 0 437 459 Size: 437 22 BsaWI w'CCGG_w Cuts at: 0 125 459 Size: 125 334 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 406 409 459 Size: 406 3 50 Fragments arranged by size: 406 50 3 BseYI C'CCAG_C Cuts at: 0 33 101 442 459 Size: 33 68 341 17 Fragments arranged by size: 341 68 33 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 235 310 459 Size: 139 96 75 149 Fragments arranged by size: 149 139 96 75 Bsp1286I G_dGCh'C Cuts at: 0 191 352 459 Size: 191 161 107 Fragments arranged by size: 191 161 107 BspEI T'CCGG_A Cuts at: 0 125 459 Size: 125 334 BsrFI r'CCGG_y Cuts at: 0 29 459 Size: 29 430 BstYI r'GATC_y Cuts at: 0 128 324 459 Size: 128 196 135 Fragments arranged by size: 196 135 128 Bsu36I CC'TnA_GG Cuts at: 0 157 459 Size: 157 302 BtgI C'CryG_G Cuts at: 0 134 191 300 424 459 Size: 134 57 109 124 35 Fragments arranged by size: 134 124 109 57 35 DrdI GACnn_nn'nnGTC Cuts at: 0 277 459 Size: 277 182 EarI CTCTTCn'nnn_ Cuts at: 0 231 459 Size: 231 228 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 459 Size: 374 85 HaeII r_GCGC'y Cuts at: 0 365 459 Size: 365 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 225 257 459 Size: 225 32 202 Fragments arranged by size: 225 202 32 MslI CAynn'nnrTG Cuts at: 0 139 459 Size: 139 320 MspA1I CmG'CkG Cuts at: 0 81 294 426 446 459 Size: 81 213 132 20 13 Fragments arranged by size: 213 132 81 20 13 NcoI C'CATG_G Cuts at: 0 134 300 459 Size: 134 166 159 Fragments arranged by size: 166 159 134 NspI r_CATG'y Cuts at: 0 387 459 Size: 387 72 PspXI vC'TCGA_Gb Cuts at: 0 169 388 459 Size: 169 219 71 Fragments arranged by size: 219 169 71 PvuII CAG'CTG Cuts at: 0 81 294 446 459 Size: 81 213 152 13 Fragments arranged by size: 213 152 81 13 SacII CC_GC'GG Cuts at: 0 427 459 Size: 427 32 SfcI C'TryA_G Cuts at: 0 378 459 Size: 378 81 SmlI C'TyrA_G Cuts at: 0 169 388 459 Size: 169 219 71 Fragments arranged by size: 219 169 71 SphI G_CATG'C Cuts at: 0 387 459 Size: 387 72 StyI C'CwwG_G Cuts at: 0 134 300 459 Size: 134 166 159 Fragments arranged by size: 166 159 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 25 459 Size: 25 434 XhoI C'TCGA_G Cuts at: 0 169 388 459 Size: 169 219 71 Fragments arranged by size: 219 169 71 Enzymes that do cut: AcuI AfeI AvaI BamHI BanII BglI BglII Bme1580I BsaAI BsaHI BsaWI BseRI BseYI BsgI Bsp1286I BspEI BsrFI BstYI Bsu36I BtgI DrdI EarI Eco57MI HaeII Hin4I MslI MspA1I NcoI NspI PspXI PvuII SacII SfcI SmlI SphI StyI TaqII XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI