(Linear) (Six-base) MAPSORT of: KIEE2759.seq Check: 4519 from: 1 to: 2346 KIEE2759.orf With 182 enzymes: SgfI * September 1, 2019 16:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1107 1547 1788 2346 Size: 1107 440 241 558 Fragments arranged by size: 1107 558 440 241 AccI GT'mk_AC Cuts at: 0 1058 1883 1931 2346 Size: 1058 825 48 415 Fragments arranged by size: 1058 825 415 48 Acc65I G'GTAC_C Cuts at: 0 734 2346 Size: 734 1612 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 499 913 1068 1225 1471 1587 1683 2307 Size: 499 414 155 157 246 116 96 624 Cuts at: 2307 2346 Size: 39 Fragments arranged by size: 624 499 414 246 157 155 116 96 39 AflIII A'CryG_T Cuts at: 0 506 896 2346 Size: 506 390 1450 Fragments arranged by size: 1450 506 390 AleI CACnn'nnGTG Cuts at: 0 802 1129 2341 2346 Size: 802 327 1212 5 Fragments arranged by size: 1212 802 327 5 AlwNI CAG_nnn'CTG Cuts at: 0 74 168 1406 1469 1565 1803 2226 2259 Size: 74 94 1238 63 96 238 423 33 Cuts at: 2259 2346 Size: 87 Fragments arranged by size: 1238 423 238 96 94 87 74 63 33 ApaLI G'TGCA_C Cuts at: 0 1153 2346 Size: 1153 1193 AvaI C'yCGr_G Cuts at: 0 1348 1544 1552 1874 2346 Size: 1348 196 8 322 472 Fragments arranged by size: 1348 472 322 196 8 BamHI G'GATC_C Cuts at: 0 23 236 2346 Size: 23 213 2110 Fragments arranged by size: 2110 213 23 BanI G'GyrC_C Cuts at: 0 603 734 1535 1680 1754 1761 2346 Size: 603 131 801 145 74 7 585 Fragments arranged by size: 801 603 585 145 131 74 7 BanII G_rGCy'C Cuts at: 0 38 179 1149 1559 2014 2346 Size: 38 141 970 410 455 332 Fragments arranged by size: 970 455 410 332 141 38 BbeI G_GCGC'C Cuts at: 0 1684 1758 2346 Size: 1684 74 588 Fragments arranged by size: 1684 588 74 BbsI GAAGACnn'nnnn_ Cuts at: 0 219 490 1962 2346 Size: 219 271 1472 384 Fragments arranged by size: 1472 384 271 219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1455 1489 2346 Size: 1455 34 857 Fragments arranged by size: 1455 857 34 BglI GCCn_nnn'nGGC Cuts at: 0 294 316 1370 1943 2346 Size: 294 22 1054 573 403 Fragments arranged by size: 1054 573 403 294 22 BglII A'GATC_T Cuts at: 0 680 848 2346 Size: 680 168 1498 Fragments arranged by size: 1498 680 168 BlpI GC'TnA_GC Cuts at: 0 1462 2346 Size: 1462 884 Bme1580I G_kGCm'C Cuts at: 0 1103 1157 1538 2174 2346 Size: 1103 54 381 636 172 Fragments arranged by size: 1103 636 381 172 54 BmgBI CAC'GTC Cuts at: 0 488 1047 2343 2346 Size: 488 559 1296 3 Fragments arranged by size: 1296 559 488 3 BmrI ACTGGGnnnn_n' Cuts at: 0 894 2346 Size: 894 1452 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 304 865 1129 1825 2227 2346 Size: 190 114 561 264 696 402 119 Fragments arranged by size: 696 561 402 264 190 119 114 Bpu10I CC'TnA_GC Cuts at: 0 178 1558 2346 Size: 178 1380 788 Fragments arranged by size: 1380 788 178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1924 2346 Size: 1924 422 BsaI GGTCTCn'nnnn_ Cuts at: 0 73 131 400 1801 2346 Size: 73 58 269 1401 545 Fragments arranged by size: 1401 545 269 73 58 BsaAI yAC'GTr Cuts at: 0 897 2133 2346 Size: 897 1236 213 Fragments arranged by size: 1236 897 213 BsaBI GATnn'nnATC Cuts at: 0 64 1435 1633 2346 Size: 64 1371 198 713 Fragments arranged by size: 1371 713 198 64 BsaHI Gr'CG_yC Cuts at: 0 474 1681 1727 1755 2346 Size: 474 1207 46 28 591 Fragments arranged by size: 1207 591 474 46 28 BsaWI w'CCGG_w Cuts at: 0 20 2346 Size: 20 2326 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 958 988 2056 2086 2200 2230 2346 Size: 958 30 1068 30 114 30 116 Fragments arranged by size: 1068 958 116 114 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 187 268 1126 2346 Size: 187 81 858 1220 Fragments arranged by size: 1220 858 187 81 BseYI C'CCAG_C Cuts at: 0 579 2259 2310 2346 Size: 579 1680 51 36 Fragments arranged by size: 1680 579 51 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 741 865 1447 2052 2346 Size: 442 299 124 582 605 294 Fragments arranged by size: 605 582 442 299 294 124 BsiEI CG_ry'CG Cuts at: 0 611 1097 1130 2346 Size: 611 486 33 1216 Fragments arranged by size: 1216 611 486 33 BsiHKAI G_wGCw'C Cuts at: 0 179 1157 1559 2132 2338 2346 Size: 179 978 402 573 206 8 Fragments arranged by size: 978 573 402 206 179 8 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1307 2346 Size: 1307 1039 Bsp1286I G_dGCh'C Cuts at: 0 38 179 1103 1149 1157 1538 1559 2014 Size: 38 141 924 46 8 381 21 455 Cuts at: 2014 2132 2174 2338 2346 Size: 118 42 164 8 Fragments arranged by size: 924 455 381 164 141 118 46 42 38 21 8 8 BspEI T'CCGG_A Cuts at: 0 20 2346 Size: 20 2326 BspHI T'CATG_A Cuts at: 0 380 1016 1028 1175 2346 Size: 380 636 12 147 1171 Fragments arranged by size: 1171 636 380 147 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 722 763 1107 1244 1547 1788 2346 Size: 722 41 344 137 303 241 558 Fragments arranged by size: 722 558 344 303 241 137 41 BsrBI CCG'CTC Cuts at: 0 641 1511 2346 Size: 641 870 835 Fragments arranged by size: 870 835 641 BsrDI GCAATG_nn' Cuts at: 0 1829 1847 2346 Size: 1829 18 499 Fragments arranged by size: 1829 499 18 BsrFI r'CCGG_y Cuts at: 0 606 2346 Size: 606 1740 BssHII G'CGCG_C Cuts at: 0 864 2346 Size: 864 1482 BssSI C'ACGA_G Cuts at: 0 112 345 1708 2346 Size: 112 233 1363 638 Fragments arranged by size: 1363 638 233 112 BstEII G'GTnAC_C Cuts at: 0 339 1119 1137 1494 2346 Size: 339 780 18 357 852 Fragments arranged by size: 852 780 357 339 18 BstXI CCAn_nnnn'nTGG Cuts at: 0 819 1131 1365 1443 1662 1737 1842 2106 Size: 819 312 234 78 219 75 105 264 Cuts at: 2106 2346 Size: 240 Fragments arranged by size: 819 312 264 240 234 219 105 78 75 BstYI r'GATC_y Cuts at: 0 23 236 417 680 848 1715 2346 Size: 23 213 181 263 168 867 631 Fragments arranged by size: 867 631 263 213 181 168 23 Bsu36I CC'TnA_GG Cuts at: 0 290 2346 Size: 290 2056 BtgI C'CryG_G Cuts at: 0 833 1124 1130 1489 2346 Size: 833 291 6 359 857 Fragments arranged by size: 857 833 359 291 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1299 2139 2346 Size: 1299 840 207 Fragments arranged by size: 1299 840 207 BtsI GCAGTG_nn' Cuts at: 0 103 1276 2346 Size: 103 1173 1070 Fragments arranged by size: 1173 1070 103 DraIII CAC_nnn'GTG Cuts at: 0 1161 2346 Size: 1161 1185 DrdI GACnn_nn'nnGTC Cuts at: 0 253 2346 Size: 253 2093 EaeI y'GGCC_r Cuts at: 0 317 608 961 1094 1127 1341 1433 1664 Size: 317 291 353 133 33 214 92 231 Cuts at: 1664 2096 2346 Size: 432 250 Fragments arranged by size: 432 353 317 291 250 231 214 133 92 33 EagI C'GGCC_G Cuts at: 0 608 1094 1127 2346 Size: 608 486 33 1219 Fragments arranged by size: 1219 608 486 33 EarI CTCTTCn'nnn_ Cuts at: 0 5 1087 1382 1426 1702 2346 Size: 5 1082 295 44 276 644 Fragments arranged by size: 1082 644 295 276 44 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1708 2092 2346 Size: 1708 384 254 Fragments arranged by size: 1708 384 254 EcoICRI GAG'CTC Cuts at: 0 177 1557 2346 Size: 177 1380 789 Fragments arranged by size: 1380 789 177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 304 499 865 913 1068 1129 1225 Size: 190 114 195 366 48 155 61 96 Cuts at: 1225 1471 1587 1683 1825 2227 2307 2346 Size: 246 116 96 142 402 80 39 Fragments arranged by size: 402 366 246 195 190 155 142 116 114 96 96 80 61 48 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 460 2346 Size: 460 1886 EcoO109I rG'GnC_Cy Cuts at: 0 295 888 1232 1548 1787 1992 2346 Size: 295 593 344 316 239 205 354 Fragments arranged by size: 593 354 344 316 295 239 205 HaeII r_GCGC'y Cuts at: 0 1684 1758 2346 Size: 1684 74 588 Fragments arranged by size: 1684 588 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1131 1163 2346 Size: 1131 32 1183 Fragments arranged by size: 1183 1131 32 HincII GTy'rAC Cuts at: 0 1596 1932 1953 2346 Size: 1596 336 21 393 Fragments arranged by size: 1596 393 336 21 HindIII A'AGCT_T Cuts at: 0 1207 2346 Size: 1207 1139 KasI G'GCGC_C Cuts at: 0 1680 1754 2346 Size: 1680 74 592 Fragments arranged by size: 1680 592 74 KpnI G_GTAC'C Cuts at: 0 738 2346 Size: 738 1608 MscI TGG'CCA Cuts at: 0 1435 2098 2346 Size: 1435 663 248 Fragments arranged by size: 1435 663 248 MslI CAynn'nnrTG Cuts at: 0 202 595 601 703 802 817 1129 1174 Size: 202 393 6 102 99 15 312 45 Cuts at: 1174 1441 1627 1660 1735 1816 2341 2346 Size: 267 186 33 75 81 525 5 Fragments arranged by size: 525 393 312 267 202 186 102 99 81 75 45 33 15 6 5 MspA1I CmG'CkG Cuts at: 0 57 741 1403 1677 1686 1752 2346 Size: 57 684 662 274 9 66 594 Fragments arranged by size: 684 662 594 274 66 57 9 NaeI GCC'GGC Cuts at: 0 608 2346 Size: 608 1738 NarI GG'CG_CC Cuts at: 0 1681 1755 2346 Size: 1681 74 591 Fragments arranged by size: 1681 591 74 NgoMIV G'CCGG_C Cuts at: 0 606 2346 Size: 606 1740 NspI r_CATG'y Cuts at: 0 510 600 2346 Size: 510 90 1746 Fragments arranged by size: 1746 510 90 PciI A'CATG_T Cuts at: 0 506 2346 Size: 506 1840 PflMI CCAn_nnn'nTGG Cuts at: 0 1050 2346 Size: 1050 1296 PfoI T'CCnGG_A Cuts at: 0 167 209 281 512 842 1106 1988 2346 Size: 167 42 72 231 330 264 882 358 Fragments arranged by size: 882 358 330 264 231 167 72 42 PmlI CAC'GTG Cuts at: 0 2133 2346 Size: 2133 213 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 433 465 1240 1272 2346 Size: 433 32 775 32 1074 Fragments arranged by size: 1074 775 433 32 32 PpuMI rG'GwC_Cy Cuts at: 0 888 1232 1548 1787 1992 2346 Size: 888 344 316 239 205 354 Fragments arranged by size: 888 354 344 316 239 205 PstI C_TGCA'G Cuts at: 0 1283 1799 2346 Size: 1283 516 547 Fragments arranged by size: 1283 547 516 SacI G_AGCT'C Cuts at: 0 179 1559 2346 Size: 179 1380 787 Fragments arranged by size: 1380 787 179 SalI G'TCGA_C Cuts at: 0 1930 2346 Size: 1930 416 SanDI GG'GwC_CC Cuts at: 0 1548 2346 Size: 1548 798 SapI GCTCTTCn'nnn_ Cuts at: 0 1087 1702 2346 Size: 1087 615 644 Fragments arranged by size: 1087 644 615 SexAI A'CCwGG_T Cuts at: 0 1481 2346 Size: 1481 865 SfcI C'TryA_G Cuts at: 0 1279 1795 1896 2346 Size: 1279 516 101 450 Fragments arranged by size: 1279 516 450 101 SfoI GGC'GCC Cuts at: 0 1682 1756 2346 Size: 1682 74 590 Fragments arranged by size: 1682 590 74 SmaI CCC'GGG Cuts at: 0 1350 1546 2346 Size: 1350 196 800 Fragments arranged by size: 1350 800 196 SmlI C'TyrA_G Cuts at: 0 1939 2346 Size: 1939 407 StuI AGG'CCT Cuts at: 0 88 2346 Size: 88 2258 StyI C'CwwG_G Cuts at: 0 1775 1979 2346 Size: 1775 204 367 Fragments arranged by size: 1775 367 204 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 97 145 790 1306 1482 2251 2346 Size: 97 48 645 516 176 769 95 Fragments arranged by size: 769 645 516 176 97 95 48 TatI w'GTAC_w Cuts at: 0 566 2346 Size: 566 1780 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1948 1980 2346 Size: 1948 32 366 Fragments arranged by size: 1948 366 32 Tth111I GACn'n_nGTC Cuts at: 0 106 433 2346 Size: 106 327 1913 Fragments arranged by size: 1913 327 106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 840 1131 1659 1818 1996 2346 Size: 840 291 528 159 178 350 Fragments arranged by size: 840 528 350 291 178 159 XmaI C'CCGG_G Cuts at: 0 1348 1544 2346 Size: 1348 196 802 Fragments arranged by size: 1348 802 196 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AleI AlwNI ApaLI AvaI BamHI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI KpnI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmlI PpiI PpuMI PstI SacI SalI SanDI SapI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BmtI BplI BsiWI BsmI BsrGI BstAPI BstBI BstZ17I ClaI CspCI DraI EcoRI EcoRV FalI FseI FspI FspAI HpaI MfeI MluI MmeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI ZraI