Restriction map for
pFN21AB9708
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BalI BamHI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseRI BsgI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BsrI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
MaeI MaeIII MamI MfeI MluI
MluNI MscI MslI MunI NcoI
NdeI NheI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tsp45I Tsp509I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 419 TCCGGA T^CCGGA
AlwI 417 GATCC GGATC(4/5)
AocI 1005 CCTCAGG CC^T[ACGT]AGG
AspI 948 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 916 CACGTG CAC^GTG
BbsI 757 GAAGAC GAAGAC(2/6)
BpmI 186 CTGGAG CTGGAG(16/14)
BpuAI 757 GAAGAC GAAGAC(2/6)
BsaAI 916 CACGTG [CT]AC^GT[GA]
BsaWI 419 TCCGGA [AT]^CCGG[AT]
BseAI 419 TCCGGA T^CCGGA
BspEI 419 TCCGGA T^CCGGA
BstAPI 312 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 128 CCAGCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 416 AGATCC [GA]^GATC[CT]
Bsu36I 1005 CCTCAGG CC^T[ACGT]AGG
DdeI 1006 CTCAG C^T[ACGT]AG
DpnII 417 GATC ^GATC
Eco81I 1005 CCTCAGG CC^T[ACGT]AGG
HinfI 766 GACTC G^A[ACGT]TC
MaeII 917 ACGT A^CGT
MboI 417 GATC ^GATC
MboII 757 GAAGA GAAGA(8/7)
MflI 416 AGATCC [GA]^GATC[CT]
MroI 419 TCCGGA T^CCGGA
MseI 362 TTAA T^TAA
NaeI 531 GCCGGC GCC^GGC
NdeII 417 GATC ^GATC
NgoMIV 531 GCCGGC G^CCGGC
NlaIII 429 CATG CATG^
NotI 985 GCGGCCGC GC^GGCCGC
PflFI 948 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 912 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 766 GACTC GAGTC(4/5)
PmaCI 916 CACGTG CAC^GTG
PmlI 916 CACGTG CAC^GTG
PstI 838 CTGCAG CTGCA^G
Sau3AI 417 GATC ^GATC
SfaNI 695 GCATC GCATC(5/9)
SfcI 838 CTGCAG C^T[GA][CT]AG
Tru9I 362 TTAA T^TAA
Tth111I 948 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 912 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 416 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 400 GGGCCC GGGCC^C
879 GGGCCC
BbeI 488 GGCGCC GGCGC^C
934 GGCGCC
BsmAI 89 GAGAC GTCTC(1/5)
199 GAGAC
BsmBI 89 GAGACG CGTCTC(1/5)
199 GAGACG
BspMI 691 ACCTGC ACCTGC(4/8)
315 GCAGGT
BsrBI 28 CCGCTC CCGCTC(-3/-3)
471 CCGCTC
BstF5I 696 CATCC GGATG(2/0)
873 CATCC
FokI 696 CATCC GGATG(9/13)
873 CATCC
KasI 488 GGCGCC G^GCGCC
934 GGCGCC
KspI 76 CCGCGG CCGC^GG
720 CCGCGG
NarI 488 GGCGCC GG^CGCC
934 GGCGCC
NruI 375 TCGCGA TCG^CGA
866 TCGCGA
PvuII 895 CAGCTG CAG^CTG
925 CAGCTG
SacII 76 CCGCGG CCGC^GG
720 CCGCGG
SfiI 11 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
297 GGCCGCGCTGGCC
SfoI 488 GGCGCC GGC^GCC
934 GGCGCC
TaqI 407 TCGA T^CGA
941 TCGA
TspRI 337 CAGTG CA[GC]TG[ACGT][ACGT]^
950 CAGTG
TthHB8I 407 TCGA T^CGA
941 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI AvaII BanI BanII BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV PpuMI Sau96I ScrFI
SmaI TseI XmaCI XmaI