Restriction map for
pFN21AB9708
(Circular) (Six-base) MAPSORT of: pFN21AB9708.seq Check: 6577 from: 1 to: 5728
pFN21AB9708 5728 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2311 2705 2311
Size: 394 5334
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3071 1937
Size: 1134 4594
Acc65I G'GTAC_C
Cuts at: 3049 3049
Size: 5728
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2599 3791 5175 5443 1632
Size: 967 1192 1384 268 1917
Fragments arranged by size:
1917 1384 1192 967 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5531
AflIII A'CryG_T
Cuts at: 1203 4627 5604 1203
Size: 3424 977 1327
Fragments arranged by size:
3424 1327 977
AgeI A'CCGG_T
Cuts at: 4559 4559
Size: 5728
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5728
AlwNI CAG_nnn'CTG
Cuts at: 2278 2851 2905 3471 5043 2278
Size: 573 54 566 1572 2963
Fragments arranged by size:
2963 1572 573 566 54
ApaI G_GGCC'C
Cuts at: 2409 2888 2409
Size: 479 5249
ApaLI G'TGCA_C
Cuts at: 3785 4941 3785
Size: 1156 4572
ApoI r'AATT_y
Cuts at: 1493 3037 3263 3415 5524 1493
Size: 1544 226 152 2109 1697
Fragments arranged by size:
2109 1697 1544 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2101 2150 2167 2199 2402 2482
Size: 73 132 26 49 17 32 203 80
Cuts at: 2482 2705 2732 2789 3053 1870
Size: 223 27 57 264 4545
Fragments arranged by size:
4545 264 223 203 132 80 73 57
49 32 27 26 17
BamHI G'GATC_C
Cuts at: 3058 3425 3058
Size: 367 5361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2493 2856 2939 3049 4504
Size: 325 217 627 706 363 83 110 1455
Cuts at: 4504 618
Size: 1842
Fragments arranged by size:
1842 1455 706 627 363 325 217 110
83
BanII G_rGCy'C
Cuts at: 1391 2174 2409 2888 3047 1391
Size: 783 235 479 159 4072
Fragments arranged by size:
4072 783 479 235 159
BbeI G_GCGC'C
Cuts at: 2497 2943 2497
Size: 446 5282
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2769 928
Size: 971 870 3887
Fragments arranged by size:
3887 971 870
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3919 3953 1903
Size: 34 50 34 1898 34 3678
Fragments arranged by size:
3678 1898 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4830 3620
Size: 1210 4518
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2023 2119 2309
Size: 107 122 71 631 605 351 96 190
Cuts at: 2309 2404 4339 136
Size: 95 1935 1525
Fragments arranged by size:
1935 1525 631 605 351 190 122 107
96 95 71
BglII A'GATC_T
Cuts at: 5723 5723
Size: 5728
BlpI GC'TnA_GC
Cuts at: 3145 3145
Size: 5728
Bme1580I G_kGCm'C
Cuts at: 1790 2409 2859 2888 3789 4945 1790
Size: 619 450 29 901 1156 2573
Fragments arranged by size:
2573 1156 901 619 450 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3459 4417 442
Size: 416 53 83 399 24 2042 958 1753
Fragments arranged by size:
2042 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3026
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2212 4388 1239
Size: 973 2176 2579
Fragments arranged by size:
2579 2176 973
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3853 4718 5016 3853
Size: 865 298 4565
Fragments arranged by size:
4565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4391 882
Size: 617 2892 2219
Fragments arranged by size:
2892 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2923 3519 493
Size: 711 1719 596 2702
Fragments arranged by size:
2702 1719 711 596
BsaBI GATnn'nnATC
Cuts at: 5722 5722
Size: 5728
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2494 2556 2643
Size: 53 83 186 936 564 397 62 87
Cuts at: 2643 2940 3535 3917 4552 275
Size: 297 595 382 635 1451
Fragments arranged by size:
1451 936 635 595 564 397 382 297
186 87 83 62 53
BsaWI w'CCGG_w
Cuts at: 2424 4161 4559 4833 4980 5391 2424
Size: 1737 398 274 147 411 2761
Fragments arranged by size:
2761 1737 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5503
Fragments arranged by size:
5503 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5728
BseYI C'CCAG_C
Cuts at: 2132 2140 2218 2313 2443 2499 2543 4931 5427
Size: 8 78 95 130 56 44 2388 496
Cuts at: 5427 2132
Size: 2433
Fragments arranged by size:
2433 2388 496 130 95 78 56 44
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2241 2304 2584 2685 2785 2814
Size: 275 186 240 63 280 101 100 29
Cuts at: 2814 2994 3178 3939 4967 1540
Size: 180 184 761 1028 2301
Fragments arranged by size:
2301 1028 761 280 275 240 186 184
180 101 100 63 29
BsiHKAI G_wGCw'C
Cuts at: 3789 3874 4945 3789
Size: 85 1071 4572
Fragments arranged by size:
4572 1071 85
BsmI GAATG_Cn'
Cuts at: 3239 3332 3239
Size: 93 5635
BsmBI CGTCTCn'nnnn_
Cuts at: 2088 2198 2088
Size: 110 5618
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2409 2859 2888 3047 3789 3874
Size: 399 384 235 450 29 159 742 85
Cuts at: 3874 4945 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 384 235 159
85 29
BspEI T'CCGG_A
Cuts at: 2424 5391 2424
Size: 2967 2761
BspHI T'CATG_A
Cuts at: 3617 5347 3617
Size: 1730 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2311 2705 3083 844
Size: 1467 394 378 3489
Fragments arranged by size:
3489 1467 394 378
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 2478 3615 1394
Size: 429 212 443 1137 3507
Fragments arranged by size:
3507 1137 443 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 4223 4397 65
Size: 1183 2975 174 1396
Fragments arranged by size:
2975 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2158 2236 2536 4372 4559 1700
Size: 110 348 78 300 1836 187 2869
Fragments arranged by size:
2869 1836 348 300 187 110 78
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5728
BssHII G'CGCG_C
Cuts at: 1925 2136 2248 2250 2503 2715 2799 1925
Size: 211 112 2 253 212 84 4854
Fragments arranged by size:
4854 253 212 211 112 84 2
BssSI C'ACGA_G
Cuts at: 3788 4800 3788
Size: 1012 4716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2323 794
Size: 1529 4199
BstBI TT'CG_AA
Cuts at: 4532 4532
Size: 5728
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5728
BstXI CCAn_nnnn'nTGG
Cuts at: 2140 2140
Size: 5728
BstYI r'GATC_y
Cuts at: 1972 2421 3058 3425 3810 3827 5279 5365 5377
Size: 449 637 367 385 17 1452 86 12
Cuts at: 5377 5723 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 637 449 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3011 3011
Size: 5728
BtgI C'CryG_G
Cuts at: 513 1065 2081 2725 2967 513
Size: 552 1016 644 242 3274
Fragments arranged by size:
3274 1016 644 552 242
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2854 487
Size: 683 56 1628 3361
Fragments arranged by size:
3361 1628 683 56
BtsI GCAGTG_nn'
Cuts at: 2348 3244 4038 4058 2348
Size: 896 794 20 4018
Fragments arranged by size:
4018 896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 3032 3032
Size: 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2301 2392
Size: 54 1425 50 275 18 408 63 91
Cuts at: 2392 2782 2811 2964 2991 3175 4064 5693 8
Size: 390 29 153 27 184 889 1629 43
Fragments arranged by size:
1629 1425 889 408 390 275 184 153
91 63 54 50 43 29 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2238 2301 2782 2811 2991 3175 1537
Size: 275 426 63 481 29 180 184 4090
Fragments arranged by size:
4090 481 426 275 184 180 63 29
EarI CTCTTCn'nnn_
Cuts at: 1353 3658 1353
Size: 2305 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4315 4689 4835 387
Size: 1064 138 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2599 3791 4388 5175 5443 1239
Size: 393 580 387 1192 597 787 268 1524
Fragments arranged by size:
1524 1192 787 597 580 393 387 268
EcoO109I rG'GnC_Cy
Cuts at: 2106 2275 2406 2736 3007 3016 2106
Size: 169 131 330 271 9 4818
Fragments arranged by size:
4818 330 271 169 131 9
EcoRI G'AATT_C
Cuts at: 3037 3415 3037
Size: 378 5350
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 2199 2497 2943 4875 1678
Size: 521 298 446 1932 2531
Fragments arranged by size:
2531 1932 521 446 298
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2756 2788 4369 4401 4443
Size: 32 135 32 1184 32 1581 32 42
Cuts at: 4443 4475 1373
Size: 32 2626
Fragments arranged by size:
2626 1581 1184 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3072 3318 669
Size: 988 281 1134 246 3079
Fragments arranged by size:
3079 1134 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5728
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KasI G'GCGC_C
Cuts at: 2493 2939 2493
Size: 446 5282
KpnI G_GTAC'C
Cuts at: 3053 3053
Size: 5728
MfeI C'AATT_G
Cuts at: 3327 3327
Size: 5728
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4842 5026 1305
Size: 3537 184 2007
Fragments arranged by size:
3537 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3906
Fragments arranged by size:
3906 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3686 4045 4204 518
Size: 648 2520 359 159 2042
Fragments arranged by size:
2520 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2566 2727 2890 2902 2932 3144 3821
Size: 477 483 161 163 12 30 212 677
Cuts at: 3821 4969 5214 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 483 477 245 212 163
161 30 12
NaeI GCC'GGC
Cuts at: 2538 2538
Size: 5728
NarI GG'CG_CC
Cuts at: 2494 2940 2494
Size: 446 5282
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5176
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NgoMIV G'CCGG_C
Cuts at: 2536 2536
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 2991 3175 2991
Size: 184 5544
NruI TCG'CGA
Cuts at: 2382 2873 2382
Size: 491 5237
NspI r_CATG'y
Cuts at: 1132 1229 3086 4631 1132
Size: 97 1857 1545 2229
Fragments arranged by size:
2229 1857 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2851 1348
Size: 1503 4225
PciI A'CATG_T
Cuts at: 4627 4627
Size: 5728
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2923 1108
Size: 1815 3913
PfoI T'CCnGG_A
Cuts at: 2259 2259
Size: 5728
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PmlI CAC'GTG
Cuts at: 2923 2923
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 5310 5342 3797
Size: 32 1481 32 4183
Fragments arranged by size:
4183 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2106 2736 3007 3016 2106
Size: 630 271 9 4818
Fragments arranged by size:
4818 630 271 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5728
PsiI TTA'TAA
Cuts at: 3298 3298
Size: 5728
PspOMI G'GGCC_C
Cuts at: 2405 2884 2405
Size: 479 5249
PstI C_TGCA'G
Cuts at: 830 2847 3080 830
Size: 2017 233 3478
Fragments arranged by size:
3478 2017 233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
PvuII CAG'CTG
Cuts at: 2902 2932 2902
Size: 30 5698
SacII CC_GC'GG
Cuts at: 2084 2728 2084
Size: 644 5084
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SanDI GG'GwC_CC
Cuts at: 2106 2736 3016 2106
Size: 630 280 4818
Fragments arranged by size:
4818 630 280
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 3976 5688 3976
Size: 1712 4016
SfcI C'TryA_G
Cuts at: 826 1045 2843 3076 4211 4892 5083 826
Size: 219 1798 233 1135 681 191 1471
Fragments arranged by size:
1798 1471 1135 681 233 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2023 2309 2023
Size: 286 5442
SfoI GGC'GCC
Cuts at: 2495 2941 2495
Size: 446 5282
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 2103 2169 2201 2404 2484 2734 3055 1872
Size: 231 66 32 203 80 250 321 4545
Fragments arranged by size:
4545 321 250 231 203 80 66 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3832 4733 4995 820
Size: 197 926 1889 901 262 1553
Fragments arranged by size:
1889 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 3086 1132
Size: 1954 3774
SrfI GCCC'GGGC
Cuts at: 2404 2404
Size: 5728
SspI AAT'ATT
Cuts at: 5 52 3652 5
Size: 47 3600 2081
Fragments arranged by size:
3600 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5728
StyI C'CwwG_G
Cuts at: 513 1065 3167 513
Size: 552 2102 3074
Fragments arranged by size:
3074 2102 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3755 3772 3925 4110 4555 325
Size: 1255 2175 17 153 185 445 1498
Fragments arranged by size:
2175 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3974 5686 96
Size: 274 80 33 51 1443 1997 1712 138
Fragments arranged by size:
1997 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5688 5720 5688
Size: 32 5696
Tth111I GACn'n_nGTC
Cuts at: 1316 2956 1316
Size: 1640 4088
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5728
XmaI C'CCGG_G
Cuts at: 1870 2101 2167 2199 2402 2482 2732 3053 1870
Size: 231 66 32 203 80 250 321 4545
Fragments arranged by size:
4545 321 250 231 203 80 66 32
XmnI GAAnn'nnTTC
Cuts at: 3857 3857
Size: 5728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NsiI PacI PspXI PsrI RsrII SacI SapI
SexAI SwaI XcmI