################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsiYI BslI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MaeIII MamI MflI MluI MluNI MroI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 623 GTAC GT^AC AspHI 3 GAGCTC G[AT]GC[AT]^C BbsI 403 GAAGAC GAAGAC(2/6) BpmI 531 CTCCAG CTGGAG(16/14) BpuAI 403 GAAGAC GAAGAC(2/6) BsiEI 46 CGGTCG CG[GA][CT]^CG BsiHKAI 3 GAGCTC G[AT]GC[AT]^C BsmAI 757 GAGAC GTCTC(1/5) BsmBI 757 GAGACG CGTCTC(1/5) BstXI 830 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 104 GCGAGC GC[ACGT]^[ACGT]GC DpnII 675 GATC ^GATC DrdI 73 GACGGTTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 604 GAATTC G^AATTC EcoT14I 584 CCAAGG C^C[AT][AT]GG FauI 101 CCCGC CCCGC(4/6) HapII 65 CCGG C^CGG HpaII 65 CCGG C^CGG HphI 166 GGTGA GGTGA(8/7) MboI 675 GATC ^GATC MfeI 497 CAATTG C^AATTG MspA1I 427 CAGCTG C[AC]G^C[GT]G MspI 65 CCGG C^CGG MunI 497 CAATTG C^AATTG NdeII 675 GATC ^GATC PpuMI 97 GGGACCC [GA]G^G[AT]CC[CT] PstI 647 CTGCAG CTGCA^G PvuII 427 CAGCTG CAG^CTG RsaI 623 GTAC GT^AC SacI 3 GAGCTC GAGCT^C Sau3AI 675 GATC ^GATC SmaI 64 CCCGGG CCC^GGG SspI 310 AATATT AAT^ATT StyI 584 CCAAGG C^C[AT][AT]GG TaqI 198 TCGA T^CGA TthHB8I 198 TCGA T^CGA XcmI 854 CCAGGCCCTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 64 CCCGGG C^CCGGG XmaI 64 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 44 CGCG CG^CG 103 CGCG AcsI 295 AAATTT [GA]^AATT[CT] 604 GAATTC ApoI 295 AAATTT [GA]^AATT[CT] 604 GAATTC AvaII 98 GGACC G^G[AT]CC 581 GGACC BbvI 649 GCAGC GCAGC(8/12) 31 GCTGC BcnI 64 CCCGG CC^[GC]GG 65 CCGGG BstUI 44 CGCG CG^CG 103 CGCG DraII 97 GGGACCC [GA]G^G[ACGT]CC[CT] 856 AGGCCCT EcoO109I 97 GGGACCC [GA]G^G[ACGT]CC[CT] 856 AGGCCCT HaeIII 824 GGCC GG^CC 857 GGCC MslI 109 CATTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 721 CATCATCATG MvnI 44 CGCG CG^CG 103 CGCG NciI 64 CCCGG CC^[GC]GG 65 CCGGG NlaIV 97 GGGACC GG[ACGT]^[ACGT]CC 98 GGACCC SfcI 647 CTGCAG C^T[GA][CT]AG 710 CTACAG SmlI 668 CTCAAG C^T[CT][GA]AG 804 CTCAAG TseI 31 GCTGC G^C[AT]GC 649 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI AvaI BanII BmyI BsaJI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI CfoI Cfr13I EarI EcoRII Fnu4HI FokI HaeII HhaI HinP1I HinfI ItaI Ksp632I MboII MnlI MseI MvaI MwoI NlaIII Sau96I ScrFI SfaNI TfiI Tru9I Tsp509I TspRI