(Circular) (Six-base) MAPSORT of: pFN21AE3115.seq Check: 1440 from: 1 to: 5578 pFN21AE3115 5578 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3388 278 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2921 1937 Size: 984 4594 Acc65I G'GTAC_C Cuts at: 2899 2899 Size: 5578 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 2676 3641 5025 5293 1632 Size: 600 444 965 1384 268 1917 Fragments arranged by size: 1917 1384 965 600 444 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 4477 5454 1203 Size: 3274 977 1327 Fragments arranged by size: 3274 1327 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 4307 4307 Size: 5578 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2136 1184 Size: 952 4626 AlwNI CAG_nnn'CTG Cuts at: 3321 4893 3321 Size: 1572 4006 ApaLI G'TGCA_C Cuts at: 3635 4791 3635 Size: 1156 4422 ApoI r'AATT_y Cuts at: 1493 2300 2609 2887 3113 3265 5374 1493 Size: 807 309 278 226 152 2109 1697 Fragments arranged by size: 2109 1697 807 309 278 226 152 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2011 2069 2700 2903 1870 Size: 73 68 58 631 203 4545 Fragments arranged by size: 4545 631 203 73 68 58 BamHI G'GATC_C Cuts at: 2908 3275 2908 Size: 367 5211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2899 4354 618 Size: 325 217 627 1112 1455 1842 Fragments arranged by size: 1842 1455 1112 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2674 2810 2897 1391 Size: 621 662 136 87 4072 Fragments arranged by size: 4072 662 621 136 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2415 928 Size: 971 516 4091 Fragments arranged by size: 4091 971 516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3769 3803 1903 Size: 34 1832 34 3678 Fragments arranged by size: 3678 1832 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3470 4680 3470 Size: 1210 4368 BclI T'GATC_A Cuts at: 1548 1548 Size: 5578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4189 136 Size: 107 122 71 631 605 2517 1525 Fragments arranged by size: 2517 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5573 5573 Size: 5578 BlpI GC'TnA_GC Cuts at: 2995 2995 Size: 5578 Bme1580I G_kGCm'C Cuts at: 1790 3639 4795 1790 Size: 1849 1156 2573 Fragments arranged by size: 2573 1849 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5578 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3309 4267 442 Size: 416 53 83 399 24 1892 958 1753 Fragments arranged by size: 1892 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2521 4238 1239 Size: 1282 1717 2579 Fragments arranged by size: 2579 1717 1282 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2658 2794 3703 4568 4866 2658 Size: 136 909 865 298 3370 Fragments arranged by size: 3370 909 865 298 136 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4241 882 Size: 617 2742 2219 Fragments arranged by size: 2742 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3369 493 Size: 711 2165 2702 Fragments arranged by size: 2702 2165 711 BsaBI GATnn'nnATC Cuts at: 5572 5572 Size: 5578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3385 3767 4402 275 Size: 53 83 186 936 1852 382 635 1451 Fragments arranged by size: 1852 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4011 4409 4683 4830 5241 4011 Size: 398 274 147 411 4348 Fragments arranged by size: 4348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2815 2845 1178 Size: 30 165 30 1412 30 3911 Fragments arranged by size: 3911 1412 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5578 BseYI C'CCAG_C Cuts at: 2098 2842 4781 5277 2098 Size: 744 1939 496 2399 Fragments arranged by size: 2399 1939 744 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5005 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2054 3028 3789 4817 1540 Size: 275 186 53 974 761 1028 2301 Fragments arranged by size: 2301 1028 974 761 275 186 53 BsiHKAI G_wGCw'C Cuts at: 2012 3639 3724 4795 2012 Size: 1627 85 1071 2795 Fragments arranged by size: 2795 1627 1071 85 BsmI GAATG_Cn' Cuts at: 3089 3182 3089 Size: 93 5485 BsmBI CGTCTCn'nnnn_ Cuts at: 2756 2756 Size: 5578 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2674 2810 2897 3639 3724 4795 Size: 399 222 662 136 87 742 85 1071 Cuts at: 4795 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 662 399 222 136 87 85 BspEI T'CCGG_A Cuts at: 5241 5241 Size: 5578 BspHI T'CATG_A Cuts at: 3467 5197 3467 Size: 1730 3848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2933 844 Size: 2089 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3465 1394 Size: 429 1642 3507 Fragments arranged by size: 3507 1642 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4073 4247 65 Size: 1183 2825 174 1396 Fragments arranged by size: 2825 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4222 4409 1700 Size: 110 2412 187 2869 Fragments arranged by size: 2869 2412 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5578 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5578 BssSI C'ACGA_G Cuts at: 3638 4650 3638 Size: 1012 4566 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5578 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5578 BstXI CCAn_nnnn'nTGG Cuts at: 2842 2842 Size: 5578 BstYI r'GATC_y Cuts at: 1972 2908 3275 3660 3677 5129 5215 5227 5573 Size: 936 367 385 17 1452 86 12 346 Cuts at: 5573 1972 Size: 1977 Fragments arranged by size: 1977 1452 936 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5026 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4839 Fragments arranged by size: 4839 683 56 BtsI GCAGTG_nn' Cuts at: 2649 3094 3888 3908 2649 Size: 445 794 20 4319 Fragments arranged by size: 4319 794 445 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5543 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2084 4585 809 Size: 507 768 2501 1802 Fragments arranged by size: 2501 1802 768 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3025 3914 5543 Size: 54 1425 50 275 18 1195 889 1629 Cuts at: 5543 8 Size: 43 Fragments arranged by size: 1629 1425 1195 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3025 1537 Size: 275 1213 4090 Fragments arranged by size: 4090 1213 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2504 2589 3508 1353 Size: 831 320 85 919 3423 Fragments arranged by size: 3423 919 831 320 85 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4165 4539 4685 387 Size: 1064 138 2576 374 146 1280 Fragments arranged by size: 2576 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 5578 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2232 2521 2676 3641 4238 5025 5293 Size: 393 600 289 155 965 597 787 268 Cuts at: 5293 1239 Size: 1524 Fragments arranged by size: 1524 965 787 600 597 393 289 268 155 EcoO109I rG'GnC_Cy Cuts at: 2103 2862 2103 Size: 759 4819 EcoRI G'AATT_C Cuts at: 2609 2887 3265 2609 Size: 278 378 4922 Fragments arranged by size: 4922 378 278 FspI TGC'GCA Cuts at: 4084 4084 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 2019 2037 2099 4725 1678 Size: 341 18 62 2626 2531 Fragments arranged by size: 2626 2531 341 62 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2185 2217 2631 2663 4219 Size: 32 135 32 613 32 414 32 1556 Cuts at: 4219 4251 4293 4325 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1556 613 414 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2922 3168 669 Size: 988 281 984 246 3079 Fragments arranged by size: 3079 988 984 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KpnI G_GTAC'C Cuts at: 2903 2903 Size: 5578 MfeI C'AATT_G Cuts at: 2502 3177 2502 Size: 675 4903 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 2603 4692 4876 1305 Size: 729 569 2089 184 2007 Fragments arranged by size: 2089 2007 729 569 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3756 Fragments arranged by size: 3756 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2118 2730 3536 3895 4054 518 Size: 648 952 612 806 359 159 2042 Fragments arranged by size: 2042 952 806 648 612 359 159 MspA1I CmG'CkG Cuts at: 1606 2434 2994 3671 4819 5064 1606 Size: 828 560 677 1148 245 2120 Fragments arranged by size: 2120 1148 828 677 560 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5026 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 3025 3025 Size: 5578 NspI r_CATG'y Cuts at: 1132 1229 2936 4481 1132 Size: 97 1707 1545 2229 Fragments arranged by size: 2229 1707 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5578 PciI A'CATG_T Cuts at: 4477 4477 Size: 5578 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5578 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3647 3679 5160 5192 3647 Size: 32 1481 32 4033 Fragments arranged by size: 4033 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2103 2103 Size: 5578 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5578 PsiI TTA'TAA Cuts at: 3148 3148 Size: 5578 PstI C_TGCA'G Cuts at: 830 2656 2930 830 Size: 1826 274 3478 Fragments arranged by size: 3478 1826 274 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 PvuII CAG'CTG Cuts at: 2434 2434 Size: 5578 SacI G_AGCT'C Cuts at: 2012 2012 Size: 5578 SalI G'TCGA_C Cuts at: 1936 2920 1936 Size: 984 4594 SanDI GG'GwC_CC Cuts at: 2103 2103 Size: 5578 SbfI CC_TGCA'GG Cuts at: 2930 2930 Size: 5578 ScaI AGT'ACT Cuts at: 3826 5538 3826 Size: 1712 3866 SfcI C'TryA_G Cuts at: 826 1045 2652 2715 2926 4061 4742 4933 826 Size: 219 1607 63 211 1135 681 191 1471 Fragments arranged by size: 1607 1471 1135 681 219 211 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5578 SmaI CCC'GGG Cuts at: 1872 2071 2905 1872 Size: 199 834 4545 Fragments arranged by size: 4545 834 199 SmlI C'TyrA_G Cuts at: 820 1017 1943 2673 2809 3682 4583 4845 820 Size: 197 926 730 136 873 901 262 1553 Fragments arranged by size: 1553 926 901 873 730 262 197 136 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5578 SphI G_CATG'C Cuts at: 1132 2936 1132 Size: 1804 3774 SspI AAT'ATT Cuts at: 5 52 2317 3502 5 Size: 47 2265 1185 2081 Fragments arranged by size: 2265 2081 1185 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5578 StyI C'CwwG_G Cuts at: 513 1065 2589 3017 513 Size: 552 1524 428 3074 Fragments arranged by size: 3074 1524 552 428 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3605 3622 3775 3960 4405 325 Size: 1255 2025 17 153 185 445 1498 Fragments arranged by size: 2025 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2627 3824 5536 Size: 274 80 33 51 1443 650 1197 1712 Cuts at: 5536 96 Size: 138 Fragments arranged by size: 1712 1443 1197 650 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2815 2847 5538 5570 2815 Size: 32 2691 32 2823 Fragments arranged by size: 2823 2691 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5578 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2866 2866 Size: 5578 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2069 2903 1870 Size: 199 834 4545 Fragments arranged by size: 4545 834 199 XmnI GAAnn'nnTTC Cuts at: 3707 3707 Size: 5578 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3386 276 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI