################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BbrPI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MluNI MscI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccIII 22 TCCGGA T^CCGGA AflIII 90 ACACGT A^C[GA][CT]GT AspI 703 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 37 GAGCCC G[GA]GC[CT]^C BbeI 282 GGCGCC GGCGC^C BbsI 73 GAAGAC GAAGAC(2/6) BcgI 375 GCAACTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 229 GCCGCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 37 GAGCCC G[AGT]GC[ACT]^C BpmI 502 CTGGAG CTGGAG(16/14) BpuAI 73 GAAGAC GAAGAC(2/6) BsaI 580 GGTCTC GGTCTC(1/5) BseAI 22 TCCGGA T^CCGGA BseRI 505 GAGGAG GAGGAG(10/8) BsiEI 154 CGGTCG CG[GA][CT]^CG BsmAI 581 GTCTC GTCTC(1/5) Bsp1286I 37 GAGCCC G[AGT]GC[ACT]^C BspEI 22 TCCGGA T^CCGGA BstF5I 252 GGATG GGATG(2/0) DraIII 766 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG FokI 252 GGATG GGATG(9/13) KasI 282 GGCGCC G^GCGCC MboII 73 GAAGA GAAGA(8/7) MroI 22 TCCGGA T^CCGGA MslI 816 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 282 GGCGCC GG^CGCC NlaIII 769 CATG CATG^ PflFI 703 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SbfI 891 CCTGCAGG CCTGCA^GG ScaI 725 AGTACT AGT^ACT SexAI 314 ACCTGGT A^CC[AT]GGT SfiI 228 GGCCGCATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 282 GGCGCC GGC^GCC SmlI 535 CTCAAG C^T[CT][GA]AG Sse8387I 891 CCTGCAGG CCTGCA^GG TfiI 336 GAATC G^A[AT]TC Tth111I 703 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 548 CCATCGTGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 282 GGCGCC G[GA]^CG[CT]C 622 GACGCC AfaI 555 GTAC GT^AC 726 GTAC AlwI 219 GATCC GGATC(4/5) 513 GGATC BanI 282 GGCGCC G^G[CT][GA]CC 318 GGTGCC BsaHI 282 GGCGCC G[GA]^CG[CT]C 622 GACGCC BsaWI 22 TCCGGA [AT]^CCGG[AT] 152 TCCGGT BsgI 835 CTGCAC GTGCAG(16/14) 163 GTGCAG BsmFI 620 GGGAC GGGAC(10/14) 106 GTCCC BsrFI 321 GCCGGT [GA]^CCGG[CT] 679 ACCGGC BsrI 279 ACTGG ACTGG(1/-1) 452 ACTGG BstYI 218 AGATCC [GA]^GATC[CT] 513 GGATCT Cfr10I 321 GCCGGT [GA]^CCGG[CT] 679 ACCGGC DdeI 379 CTGAG C^T[ACGT]AG 787 CTGAG DsaI 223 CCGCGG C^C[GA][CT]GG 626 CCGCGG Hin1I 282 GGCGCC G[GA]^CG[CT]C 622 GACGCC HincII 604 GTCAAC GT[CT]^[GA]AC 886 GTCAAC HindII 604 GTCAAC GT[CT]^[GA]AC 886 GTCAAC KspI 223 CCGCGG CCGC^GG 626 CCGCGG MaeIII 290 GTCAC ^GT[ACGT]AC 449 GTGAC MflI 218 AGATCC [GA]^GATC[CT] 513 GGATCT PflMI 765 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 840 CCAGGCCCTGG PleI 381 GAGTC GAGTC(4/5) 778 GAGTC PpuMI 104 GGGTCCC [GA]G^G[AT]CC[CT] 311 AGGACCT PvuII 118 CAGCTG CAG^CTG 343 CAGCTG RsaI 555 GTAC GT^AC 726 GTAC SacII 223 CCGCGG CCGC^GG 626 CCGCGG SfaNI 196 GATGC GCATC(5/9) 253 GATGC TaqI 194 TCGA T^CGA 818 TCGA Tsp45I 290 GTCAC ^GT[GC]AC 449 GTGAC TthHB8I 194 TCGA T^CGA 818 TCGA Van91I 765 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 840 CCAGGCCCTGG XhoII 218 AGATCC [GA]^GATC[CT] 513 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AluI AlwNI AvaII BbvI BcnI BsaJI BsiYI BslI BspMI BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfcI TseI TspRI