(Circular) (Six-base) MAPSORT of: pFN21AE4053.seq Check: 2980 from: 1 to: 5605 pFN21AE4053 5605 bp With 182 enzymes: SgfI * March 28, 2012 13:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2200 2200 Size: 5605 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2948 1937 Size: 1011 4594 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3668 5052 5320 1632 Size: 2036 1384 268 1917 Fragments arranged by size: 2036 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 2095 4504 5481 1203 Size: 892 2409 977 1327 Fragments arranged by size: 2409 1327 977 892 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5605 AlwNI CAG_nnn'CTG Cuts at: 2278 2437 2851 3348 4920 2278 Size: 159 414 497 1572 2963 Fragments arranged by size: 2963 1572 497 414 159 ApaLI G'TGCA_C Cuts at: 3662 4818 3662 Size: 1156 4449 ApoI r'AATT_y Cuts at: 1493 2914 3140 3292 5401 1493 Size: 1421 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 226 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2930 1870 Size: 73 987 4545 Fragments arranged by size: 4545 987 73 BamHI G'GATC_C Cuts at: 2935 3302 2935 Size: 367 5238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2287 2323 2926 4381 618 Size: 325 217 627 500 36 603 1455 1842 Fragments arranged by size: 1842 1455 627 603 500 325 217 36 BanII G_rGCy'C Cuts at: 1391 2046 2924 1391 Size: 655 878 4072 Fragments arranged by size: 4072 878 655 BbeI G_GCGC'C Cuts at: 2291 2291 Size: 5605 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2085 928 Size: 971 186 4448 Fragments arranged by size: 4448 971 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2369 2403 3796 3830 1903 Size: 34 432 34 1393 34 3678 Fragments arranged by size: 3678 1393 432 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3497 4707 3497 Size: 1210 4395 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2240 4216 136 Size: 107 122 71 631 605 568 1976 1525 Fragments arranged by size: 1976 1525 631 605 568 122 107 71 BglII A'GATC_T Cuts at: 5600 5600 Size: 5605 BlpI GC'TnA_GC Cuts at: 3022 3022 Size: 5605 Bme1580I G_kGCm'C Cuts at: 1790 3666 4822 1790 Size: 1876 1156 2573 Fragments arranged by size: 2573 1876 1156 BmgBI CAC'GTC Cuts at: 1318 2083 2098 2827 1318 Size: 765 15 729 4096 Fragments arranged by size: 4096 765 729 15 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2467 3336 4294 Size: 416 53 83 399 24 1050 869 958 Cuts at: 4294 442 Size: 1753 Fragments arranged by size: 1753 1050 958 869 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2528 4265 1239 Size: 1289 1737 2579 Fragments arranged by size: 2579 1737 1289 Bpu10I CC'TnA_GC Cuts at: 2792 2792 Size: 5605 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 3730 4595 4893 2525 Size: 1205 865 298 3237 Fragments arranged by size: 3237 1205 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2591 4268 882 Size: 617 1092 1677 2219 Fragments arranged by size: 2219 1677 1092 617 BsaAI yAC'GTr Cuts at: 493 1204 3396 493 Size: 711 2192 2702 Fragments arranged by size: 2702 2192 711 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2288 2628 3412 3794 Size: 53 83 186 936 755 340 784 382 Cuts at: 3794 4429 275 Size: 635 1451 Fragments arranged by size: 1451 936 784 755 635 382 340 186 83 53 BsaWI w'CCGG_w Cuts at: 2027 2157 4038 4436 4710 4857 5268 2027 Size: 130 1881 398 274 147 411 2364 Fragments arranged by size: 2364 1881 411 398 274 147 130 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5380 Fragments arranged by size: 5380 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2525 1186 Size: 1339 4266 BseYI C'CCAG_C Cuts at: 4808 5304 4808 Size: 496 5109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2189 2825 1163 Size: 573 453 636 3943 Fragments arranged by size: 3943 636 573 453 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2162 3055 3816 4844 1540 Size: 275 186 161 893 761 1028 2301 Fragments arranged by size: 2301 1028 893 761 275 186 161 BsiHKAI G_wGCw'C Cuts at: 3666 3751 4822 3666 Size: 85 1071 4449 Fragments arranged by size: 4449 1071 85 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2924 3666 3751 4822 1391 Size: 399 256 878 742 85 1071 2174 Fragments arranged by size: 2174 1071 878 742 399 256 85 BspEI T'CCGG_A Cuts at: 2027 5268 2027 Size: 3241 2364 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 2200 2890 2904 2960 844 Size: 1232 124 690 14 56 3489 Fragments arranged by size: 3489 1232 690 124 56 14 BsrBI CCG'CTC Cuts at: 1394 1823 3492 1394 Size: 429 1669 3507 Fragments arranged by size: 3507 1669 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4100 4274 65 Size: 1183 2852 174 1396 Fragments arranged by size: 2852 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2326 2684 4249 4436 1700 Size: 110 516 358 1565 187 2869 Fragments arranged by size: 2869 1565 516 358 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5605 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2223 2518 2935 3302 3687 3704 5156 5242 Size: 251 295 417 367 385 17 1452 86 Cuts at: 5242 5254 5600 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 417 385 367 346 295 251 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2228 2631 513 Size: 552 939 224 403 3487 Fragments arranged by size: 3487 939 552 403 224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 2764 3121 3915 3935 2764 Size: 357 794 20 4434 Fragments arranged by size: 4434 794 357 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DraIII CAC_nnn'GTG Cuts at: 2776 2776 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4612 809 Size: 507 3296 1802 Fragments arranged by size: 3296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2409 2604 Size: 54 1425 50 275 18 351 228 195 Cuts at: 2604 2724 3052 3941 5570 8 Size: 120 328 889 1629 43 Fragments arranged by size: 1629 1425 889 351 328 275 228 195 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3535 1353 Size: 2182 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2795 4192 4566 4712 387 Size: 1064 138 1206 1397 374 146 1280 Fragments arranged by size: 1397 1280 1206 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2528 3668 4265 5052 5320 1239 Size: 393 896 1140 597 787 268 1524 Fragments arranged by size: 1524 1140 896 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2110 2317 2848 2110 Size: 207 531 4867 Fragments arranged by size: 4867 531 207 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 2291 2399 2437 2477 2583 4752 1678 Size: 613 108 38 40 106 2169 2531 Fragments arranged by size: 2531 2169 613 108 106 40 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4246 4278 4320 4352 1373 Size: 32 135 32 2674 32 42 32 2626 Fragments arranged by size: 2674 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2611 2893 2949 3195 669 Size: 988 281 673 282 56 246 3079 Fragments arranged by size: 3079 988 673 282 281 246 56 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KasI G'GCGC_C Cuts at: 2287 2287 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2680 4719 4903 1305 Size: 1375 2039 184 2007 Fragments arranged by size: 2039 2007 1375 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3783 Fragments arranged by size: 3783 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2825 3563 3922 4081 518 Size: 648 1659 738 359 159 2042 Fragments arranged by size: 2042 1659 738 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2116 2125 2230 2350 2633 2839 3021 3698 Size: 510 9 105 120 283 206 182 677 Cuts at: 3698 4846 5091 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 510 283 245 206 182 120 105 9 NarI GG'CG_CC Cuts at: 2288 2288 Size: 5605 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4114 Fragments arranged by size: 4114 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2963 4508 1132 Size: 97 1734 1545 2229 Fragments arranged by size: 2229 1734 1545 97 PasI CC'CwG_GG Cuts at: 1348 2073 1348 Size: 725 4880 PciI A'CATG_T Cuts at: 4504 4504 Size: 5605 PflMI CCAn_nnn'nTGG Cuts at: 1108 2776 2851 1108 Size: 1668 75 3862 Fragments arranged by size: 3862 1668 75 PfoI T'CCnGG_A Cuts at: 2061 2061 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2110 2317 2110 Size: 207 5398 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PstI C_TGCA'G Cuts at: 830 2139 2274 2433 2865 2901 2957 830 Size: 1309 135 159 432 36 56 3478 Fragments arranged by size: 3478 1309 432 159 135 56 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 PvuII CAG'CTG Cuts at: 2125 2350 2125 Size: 225 5380 SacII CC_GC'GG Cuts at: 2231 2634 2231 Size: 403 5202 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SanDI GG'GwC_CC Cuts at: 2110 2110 Size: 5605 SbfI CC_TGCA'GG Cuts at: 2901 2957 2901 Size: 56 5549 ScaI AGT'ACT Cuts at: 2732 3853 5565 2732 Size: 1121 1712 2772 Fragments arranged by size: 2772 1712 1121 SexAI A'CCwGG_T Cuts at: 2319 2319 Size: 5605 SfcI C'TryA_G Cuts at: 826 1045 2135 2270 2429 2861 2897 2953 4088 Size: 219 1090 135 159 432 36 56 1135 Cuts at: 4088 4769 4960 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1090 681 432 219 191 159 135 56 36 SfiI GGCCn_nnn'nGGCC Cuts at: 2240 2240 Size: 5605 SfoI GGC'GCC Cuts at: 2289 2289 Size: 5605 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5605 SmaI CCC'GGG Cuts at: 1872 2932 1872 Size: 1060 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 3709 4610 4872 820 Size: 197 926 597 1169 901 262 1553 Fragments arranged by size: 1553 1169 926 901 597 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5605 StyI C'CwwG_G Cuts at: 513 1065 2004 3044 513 Size: 552 939 1040 3074 Fragments arranged by size: 3074 1040 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2449 3632 3649 3802 3987 4432 325 Size: 1255 869 1183 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1183 869 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2730 3851 5563 Size: 274 80 33 51 1443 753 1121 1712 Cuts at: 5563 96 Size: 138 Fragments arranged by size: 1712 1443 1121 753 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5565 5597 5565 Size: 32 5573 Tth111I GACn'n_nGTC Cuts at: 1316 2711 1316 Size: 1395 4210 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2560 2560 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2930 1870 Size: 1060 4545 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SrfI SwaI