Restriction map for
pFN21AB8930
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BlnI BsaAI BsaI BseAI
BsgI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MfeI MluI
MluNI MroI MscI MslI MunI
NcoI NdeI NheI NlaIII NotI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AfaI 444 GTAC GT^AC
AhdI 568 GACGTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 478 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 425 AGCGCT AGC^GCT
ApaI 591 GGGCCC GGGCC^C
AspEI 568 GACGTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 268 GAGCTC G[AT]GC[AT]^C
BbsI 817 GAAGAC GAAGAC(2/6)
BglI 721 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 407 AGATCT A^GATCT
BlpI 532 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 532 GCTAAGC GC^T[ACGT]AGC
BpuAI 817 GAAGAC GAAGAC(2/6)
BsaBI 606 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 702 TCCGGT [AT]^CCGG[AT]
BsiEI 459 CGATCG CG[GA][CT]^CG
BsiHKAI 268 GAGCTC G[AT]GC[AT]^C
BsrI 588 ACTGG ACTGG(1/-1)
BstF5I 120 CATCC GGATG(2/0)
BstXI 581 CCAAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 407 AGATCT [GA]^GATC[CT]
CelII 532 GCTAAGC GC^T[ACGT]AGC
DsaI 185 CCGCGG C^C[GA][CT]GG
Eam1105I 568 GACGTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 823 GAAGAG CTCTTC(1/4)
Eco47III 425 AGCGCT AGC^GCT
EcoT14I 8 CCAAGG C^C[AT][AT]GG
FokI 120 CATCC GGATG(9/13)
HgaI 99 GACGC GACGC(5/10)
Ksp632I 823 GAAGAG CTCTTC(1/4)
KspI 185 CCGCGG CCGC^GG
MaeIII 149 GTTAC ^GT[ACGT]AC
MamI 606 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 407 AGATCT [GA]^GATC[CT]
MseI 287 TTAA T^TAA
NruI 462 TCGCGA TCG^CGA
PaeR7I 631 CTCGAG C^TCGAG
PleI 574 GAGTC GAGTC(4/5)
PpuMI 545 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 459 CGATCG CGAT^CG
PvuII 481 CAGCTG CAG^CTG
RsaI 444 GTAC GT^AC
SacI 268 GAGCTC GAGCT^C
SacII 185 CCGCGG CCGC^GG
SexAI 511 ACCTGGT A^CC[AT]GGT
SfaNI 110 GCATC GCATC(5/9)
SmaI 732 CCCGGG CCC^GGG
StyI 8 CCAAGG C^C[AT][AT]GG
TfiI 522 GAATC G^A[AT]TC
Tru9I 287 TTAA T^TAA
Tsp509I 416 AATT ^AATT
TspRI 95 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 596 CCAGCGGGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 631 CTCGAG C^TCGAG
XhoII 407 AGATCT [GA]^GATC[CT]
XmaCI 732 CCCGGG C^CCGGG
XmaI 732 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaI 631 CTCGAG C^[CT]CG[GA]G
732 CCCGGG
BanII 268 GAGCTC G[GA]GC[CT]^C
591 GGGCCC
BmyI 268 GAGCTC G[AGT]GC[ACT]^C
591 GGGCCC
BsmFI 545 GGGAC GGGAC(10/14)
450 GTCCC
BsoBI 631 CTCGAG C^[CT]CG[GA]G
732 CCCGGG
Bsp1286I 268 GAGCTC G[AGT]GC[ACT]^C
591 GGGCCC
BspMI 65 ACCTGC ACCTGC(4/8)
173 ACCTGC
BsrBI 769 CCGCTC CCGCTC(-3/-3)
40 GAGCGG
BsrFI 655 GCCGGC [GA]^CCGG[CT]
783 GCCGGC
Cfr10I 655 GCCGGC [GA]^CCGG[CT]
783 GCCGGC
DdeI 365 CTAAG C^T[ACGT]AG
533 CTAAG
HinfI 522 GAATC G^A[ACGT]TC
574 GAGTC
MboII 817 GAAGA GAAGA(8/7)
823 GAAGA
NaeI 655 GCCGGC GCC^GGC
783 GCCGGC
NgoMIV 655 GCCGGC G^CCGGC
783 GCCGGC
PstI 310 CTGCAG CTGCA^G
748 CTGCAG
SfcI 310 CTGCAG C^T[GA][CT]AG
748 CTGCAG
SmlI 538 CTCAAG C^T[CT][GA]AG
631 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BbeI BbvI BcnI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI KasI MaeII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI SfoI
TaqI TseI TthHB8I