(Circular) (Six-base) MAPSORT of: pFN21AB8930.seq Check: 3288 from: 1 to: 5548 pFN21AB8930 5548 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2079 2187 2079 Size: 108 5440 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3358 278 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2891 1937 Size: 954 4594 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3611 4995 5263 1632 Size: 1979 1384 268 1917 Fragments arranged by size: 1979 1917 1384 268 AfeI AGC'GCT Cuts at: 2432 2432 Size: 5548 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 4447 5424 1203 Size: 3244 977 1327 Fragments arranged by size: 3244 1327 977 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 2578 4277 2578 Size: 1699 3849 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5548 AlwNI CAG_nnn'CTG Cuts at: 2488 3291 4863 2488 Size: 803 1572 3173 Fragments arranged by size: 3173 1572 803 ApaI G_GGCC'C Cuts at: 2600 2600 Size: 5548 ApaLI G'TGCA_C Cuts at: 3605 4761 3605 Size: 1156 4392 ApoI r'AATT_y Cuts at: 1493 2857 3083 3235 5344 1493 Size: 1364 226 152 2109 1697 Fragments arranged by size: 2109 1697 1364 226 152 AseI AT'TA_AT Cuts at: 160 1032 4102 160 Size: 872 3070 1606 Fragments arranged by size: 3070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2636 2737 2873 1870 Size: 73 693 101 136 4545 Fragments arranged by size: 4545 693 136 101 73 BamHI G'GATC_C Cuts at: 2878 3245 2878 Size: 367 5181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 2723 2747 2869 4324 Size: 325 217 627 245 691 24 122 1455 Cuts at: 4324 618 Size: 1842 Fragments arranged by size: 1842 1455 691 627 325 245 217 122 24 BanII G_rGCy'C Cuts at: 1391 2277 2600 2867 1391 Size: 886 323 267 4072 Fragments arranged by size: 4072 886 323 267 BbeI G_GCGC'C Cuts at: 2036 2727 2751 2036 Size: 691 24 4833 Fragments arranged by size: 4833 691 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2829 928 Size: 971 930 3647 Fragments arranged by size: 3647 971 930 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3739 3773 1903 Size: 34 1802 34 3678 Fragments arranged by size: 3678 1802 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3440 4650 3440 Size: 1210 4338 BclI T'GATC_A Cuts at: 1548 1548 Size: 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2732 4159 136 Size: 107 122 71 631 605 1060 1427 1525 Fragments arranged by size: 1525 1427 1060 631 605 122 107 71 BglII A'GATC_T Cuts at: 2412 5543 2412 Size: 3131 2417 BlpI GC'TnA_GC Cuts at: 2538 2965 2538 Size: 427 5121 Bme1580I G_kGCm'C Cuts at: 1790 2600 3609 4765 1790 Size: 810 1009 1156 2573 Fragments arranged by size: 2573 1156 1009 810 BmgBI CAC'GTC Cuts at: 1318 2839 1318 Size: 1521 4027 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2603 3279 4237 Size: 416 53 83 399 24 1186 676 958 Cuts at: 4237 442 Size: 1753 Fragments arranged by size: 1753 1186 958 676 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2662 2662 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2288 2321 2576 4208 1239 Size: 1049 33 255 1632 2579 Fragments arranged by size: 2579 1632 1049 255 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2528 3673 4538 4836 2528 Size: 1145 865 298 3240 Fragments arranged by size: 3240 1145 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4211 882 Size: 617 2712 2219 Fragments arranged by size: 2712 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 2615 5542 2615 Size: 2927 2621 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2033 2105 2724 2748 Size: 53 83 186 936 500 72 619 24 Cuts at: 2748 3355 3737 4372 275 Size: 607 382 635 1451 Fragments arranged by size: 1451 936 635 619 607 500 382 186 83 72 53 24 BsaWI w'CCGG_w Cuts at: 2707 3981 4379 4653 4800 5211 2707 Size: 1274 398 274 147 411 3044 Fragments arranged by size: 3044 1274 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5323 Fragments arranged by size: 5323 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2285 2573 1186 Size: 871 228 288 4161 Fragments arranged by size: 4161 871 288 228 BseYI C'CCAG_C Cuts at: 2488 2600 4751 5247 2488 Size: 112 2151 496 2789 Fragments arranged by size: 2789 2151 496 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4975 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2467 2998 3759 4787 1540 Size: 275 186 466 531 761 1028 2301 Fragments arranged by size: 2301 1028 761 531 466 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 3609 3694 4765 2277 Size: 1332 85 1071 3060 Fragments arranged by size: 3060 1332 1071 85 BsmI GAATG_Cn' Cuts at: 3059 3152 3059 Size: 93 5455 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2600 2867 3609 3694 4765 1391 Size: 399 487 323 267 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 399 323 267 85 BspEI T'CCGG_A Cuts at: 5211 5211 Size: 5548 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 2187 2903 844 Size: 1235 108 716 3489 Fragments arranged by size: 3489 1235 716 108 BsrBI CCG'CTC Cuts at: 1394 1823 2047 2776 3435 1394 Size: 429 224 729 659 3507 Fragments arranged by size: 3507 729 659 429 224 BsrDI GCAATG_nn' Cuts at: 65 1248 4043 4217 65 Size: 1183 2795 174 1396 Fragments arranged by size: 2795 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2660 2788 4192 4379 1700 Size: 110 850 128 1404 187 2869 Fragments arranged by size: 2869 1404 850 187 128 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5548 BssHII G'CGCG_C Cuts at: 1925 2232 2262 2533 1925 Size: 307 30 271 4940 Fragments arranged by size: 4940 307 271 30 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5548 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstXI CCAn_nnnn'nTGG Cuts at: 2593 2593 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2412 2878 3245 3630 3647 5099 5185 5197 Size: 440 466 367 385 17 1452 86 12 Cuts at: 5197 5543 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 466 440 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2190 513 Size: 552 1125 3871 Fragments arranged by size: 3871 1125 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4809 Fragments arranged by size: 4809 683 56 BtsI GCAGTG_nn' Cuts at: 3064 3858 3878 3064 Size: 794 20 4734 Fragments arranged by size: 4734 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2628 2790 Size: 54 1425 50 275 18 354 444 162 Cuts at: 2790 2995 3884 5513 8 Size: 205 889 1629 43 Fragments arranged by size: 1629 1425 889 444 354 275 205 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2995 1537 Size: 275 1183 4090 Fragments arranged by size: 4090 1183 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2823 3478 1353 Size: 1470 655 3423 Fragments arranged by size: 3423 1470 655 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2067 2572 4135 4509 4655 387 Size: 1064 138 478 505 1563 374 146 1280 Fragments arranged by size: 1563 1280 1064 505 478 374 146 138 EcoICRI GAG'CTC Cuts at: 2275 2275 Size: 5548 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2321 2576 3611 4208 4995 5263 Size: 393 656 33 255 1035 597 787 268 Cuts at: 5263 1239 Size: 1524 Fragments arranged by size: 1524 1035 787 656 597 393 268 255 33 EcoO109I rG'GnC_Cy Cuts at: 2551 2597 2734 2551 Size: 46 137 5365 Fragments arranged by size: 5365 137 46 EcoRI G'AATT_C Cuts at: 2857 3235 2857 Size: 378 5170 FspI TGC'GCA Cuts at: 4054 4054 Size: 5548 HaeII r_GCGC'y Cuts at: 1678 2036 2434 2627 2727 2745 2751 4695 1678 Size: 358 398 193 100 18 6 1944 2531 Fragments arranged by size: 2531 1944 398 358 193 100 18 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 4189 4221 4263 Size: 32 135 32 992 32 1593 32 42 Cuts at: 4263 4295 1373 Size: 32 2626 Fragments arranged by size: 2626 1593 992 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2892 3138 669 Size: 988 281 954 246 3079 Fragments arranged by size: 3079 988 954 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5548 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KasI G'GCGC_C Cuts at: 2032 2723 2747 2032 Size: 691 24 4833 Fragments arranged by size: 4833 691 24 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2115 4662 4846 1305 Size: 810 2547 184 2007 Fragments arranged by size: 2547 2007 810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3726 Fragments arranged by size: 3726 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3506 3865 4024 518 Size: 648 2340 359 159 2042 Fragments arranged by size: 2340 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 2192 2257 2359 2488 2604 2964 3641 Size: 456 130 65 102 129 116 360 677 Cuts at: 3641 4789 5034 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 456 360 245 130 129 116 102 65 NaeI GCC'GGC Cuts at: 2662 2790 2662 Size: 128 5420 NarI GG'CG_CC Cuts at: 2033 2724 2748 2033 Size: 691 24 4833 Fragments arranged by size: 4833 691 24 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4996 NdeI CA'TA_TG Cuts at: 387 387 Size: 5548 NgoMIV G'CCGG_C Cuts at: 2660 2788 2660 Size: 128 5420 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NruI TCG'CGA Cuts at: 2469 2469 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2906 4451 1132 Size: 97 1677 1545 2229 Fragments arranged by size: 2229 1677 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5548 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5548 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5130 5162 3617 Size: 32 1481 32 4003 Fragments arranged by size: 4003 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2551 2551 Size: 5548 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PspOMI G'GGCC_C Cuts at: 2596 2596 Size: 5548 PspXI vC'TCGA_Gb Cuts at: 2636 2636 Size: 5548 PstI C_TGCA'G Cuts at: 830 2319 2757 2900 830 Size: 1489 438 143 3478 Fragments arranged by size: 3478 1489 438 143 PvuI CG_AT'CG Cuts at: 2001 2467 2001 Size: 466 5082 PvuII CAG'CTG Cuts at: 2488 2488 Size: 5548 SacI G_AGCT'C Cuts at: 2277 2277 Size: 5548 SacII CC_GC'GG Cuts at: 2193 2193 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 3796 5508 3796 Size: 1712 3836 SexAI A'CCwGG_T Cuts at: 2516 2516 Size: 5548 SfcI C'TryA_G Cuts at: 826 1045 2315 2753 2896 4031 4712 4903 826 Size: 219 1270 438 143 1135 681 191 1471 Fragments arranged by size: 1471 1270 1135 681 438 219 191 143 SfoI GGC'GCC Cuts at: 2034 2725 2749 2034 Size: 691 24 4833 Fragments arranged by size: 4833 691 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2739 2875 1872 Size: 867 136 4545 Fragments arranged by size: 4545 867 136 SmlI C'TyrA_G Cuts at: 820 1017 1943 2543 2636 3652 4553 4815 820 Size: 197 926 600 93 1016 901 262 1553 Fragments arranged by size: 1553 1016 926 901 600 262 197 93 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5548 StyI C'CwwG_G Cuts at: 513 1065 2013 2987 513 Size: 552 948 974 3074 Fragments arranged by size: 3074 974 948 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3575 3592 3745 3930 4375 325 Size: 1255 1995 17 153 185 445 1498 Fragments arranged by size: 1995 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3794 5506 96 Size: 274 80 33 51 1443 1817 1712 138 Fragments arranged by size: 1817 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5508 5540 5508 Size: 32 5516 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5548 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2608 2608 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 2636 1943 Size: 693 4855 XmaI C'CCGG_G Cuts at: 1870 2737 2873 1870 Size: 867 136 4545 Fragments arranged by size: 4545 867 136 XmnI GAAnn'nnTTC Cuts at: 3677 3677 Size: 5548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3356 276 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NsiI PacI PfoI PmlI PsrI RsrII SanDI SapI SfiI SrfI SwaI