################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeIII MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AciI 898 GCGG CCGC(-3/-1) AclI 776 AACGTT AA^CGTT AflIII 586 ACATGT A^C[GA][CT]GT ApaI 191 GGGCCC GGGCC^C AseI 622 ATTAAT AT^TAAT AsnI 622 ATTAAT AT^TAAT Asp700I 775 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 480 GTGCTC G[AT]GC[AT]^C BalI 318 TGGCCA TGG^CCA BsaI 259 GAGACC GGTCTC(1/5) BsaWI 176 TCCGGT [AT]^CCGG[AT] BsiHKAI 480 GTGCTC G[AT]GC[AT]^C BsmAI 259 GAGAC GTCTC(1/5) BsmFI 233 GGGAC GGGAC(10/14) BsmI 278 GCATTC GAATGC(1/-1) BspLU11I 586 ACATGT A^CATGT BspMI 878 GCAGGT ACCTGC(4/8) BstBI 380 TTCGAA TT^CGAA BstYI 575 AGATCC [GA]^GATC[CT] DraI 951 TTTAAA TTT^AAA HgaI 12 GCGTC GACGC(5/10) HincII 499 GTTGAC GT[CT]^[GA]AC HindII 499 GTTGAC GT[CT]^[GA]AC HindIII 529 AAGCTT A^AGCTT HphI 1019 TCACC GGTGA(8/7) MaeII 777 ACGT A^CGT MflI 575 AGATCC [GA]^GATC[CT] MluNI 318 TGGCCA TGG^CCA MscI 318 TGGCCA TGG^CCA MslI 325 CATCGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 896 CAGCGG C[AC]G^C[GT]G NdeI 724 CATATG CA^TATG NspV 380 TTCGAA TT^CGAA PshBI 622 ATTAAT AT^TAAT Psp1406I 776 AACGTT AA^CGTT ScaI 468 AGTACT AGT^ACT SfuI 380 TTCGAA TT^CGAA SphI 828 GCATGC GCATG^C SspI 946 AATATT AAT^ATT XhoII 575 AGATCC [GA]^GATC[CT] XmnI 775 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 180 GTCTAC GT^[AC][GT]AC 1024 GTAGAC AcsI 616 GAATTC [GA]^AATT[CT] 993 GAATTC AfaI 469 GTAC GT^AC 882 GTAC ApoI 616 GAATTC [GA]^AATT[CT] 993 GAATTC BanII 133 GAGCCC G[GA]GC[CT]^C 191 GGGCCC BbvI 390 GCAGC GCAGC(8/12) 506 GCAGC BfaI 239 CTAG C^TAG 649 CTAG BsaJI 116 CCATGG C^C[ACGT][ACGT]GG 485 CCATGG BstF5I 548 GGATG GGATG(2/0) 702 GGATG Cfr13I 191 GGGCC G^G[ACGT]CC 192 GGCCC DdeI 451 CTGAG C^T[ACGT]AG 1010 CTCAG DraII 190 AGGGCCC [GA]G^G[ACGT]CC[CT] 191 GGGCCCC DsaI 116 CCATGG C^C[GA][CT]GG 485 CCATGG EarI 94 GAAGAG CTCTTC(1/4) 571 GAAGAG EcoO109I 190 AGGGCCC [GA]G^G[ACGT]CC[CT] 191 GGGCCCC EcoRI 616 GAATTC G^AATTC 993 GAATTC EcoT14I 116 CCATGG C^C[AT][AT]GG 485 CCATGG Fnu4HI 390 GCAGC GC^[ACGT]GC 506 GCAGC FokI 548 GGATG GGATG(9/13) 702 GGATG HapII 39 CCGG C^CGG 177 CCGG HpaII 39 CCGG C^CGG 177 CCGG ItaI 390 GCAGC GC^[ACGT]GC 506 GCAGC Ksp632I 94 GAAGAG CTCTTC(1/4) 571 GAAGAG MaeI 239 CTAG C^TAG 649 CTAG MseI 623 TTAA T^TAA 952 TTAA MspI 39 CCGG C^CGG 177 CCGG MwoI 12 GCGTCCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 204 GCTCTATAAGC NcoI 116 CCATGG C^CATGG 485 CCATGG NspI 586 ACATGT [GA]CATG^[CT] 828 GCATGC RsaI 469 GTAC GT^AC 882 GTAC Sau96I 191 GGGCC G^G[ACGT]CC 192 GGCCC SfcI 598 CTACAG C^T[GA][CT]AG 833 CTACAG StyI 116 CCATGG C^C[AT][AT]GG 485 CCATGG Tru9I 623 TTAA T^TAA 952 TTAA TseI 390 GCAGC G^C[AT]GC 506 GCAGC TspRI 315 CACTG CA[GC]TG[ACGT][ACGT]^ 604 CAGTG XcmI 321 CCAGCATCGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 723 CCATATGAAAGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AluI AlwI BmyI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I DpnII EaeI EcoRII HaeIII HinfI MboI MboII MnlI MvaI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI TaqI TfiI Tsp509I TthHB8I