Restriction map for
pFN21AE2540
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglII BlnI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspEI BspHI BspMI BsrBI
BsrGI BssSI Bst1107I BstBI BstZ17I
Bsu36I CpoI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoT22I
FseI HgaI Hin1I HpaI KasI
KpnI KspI MluI MroI NarI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 994 GTCTAC GT^[AC][GT]AC
AflIII 435 ACATGT A^C[GA][CT]GT
AgeI 654 ACCGGT A^CCGGT
Aor51HI 146 AGCGCT AGC^GCT
ApaI 2141 GGGCCC GGGCC^C
Asp700I 1895 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 584 CCCGAG C^[CT]CG[GA]G
AviII 1113 TGCGCA TGC^GCA
BalI 268 TGGCCA TGG^CCA
BamHI 1797 GGATCC G^GATCC
BclI 362 TGATCA T^GATCA
BlpI 833 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 833 GCTTAGC GC^T[ACGT]AGC
BsaBI 2725 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 121 CTGCAC GTGCAG(16/14)
BsoBI 584 CCCGAG C^[CT]CG[GA]G
BspDI 2695 ATCGAT AT^CGAT
BspLU11I 435 ACATGT A^CATGT
BssHII 2097 GCGCGC G^CGCGC
BstAPI 2113 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1263 GGTTACC G^GT[ACGT]ACC
BstPI 1263 GGTTACC G^GT[ACGT]ACC
BstXI 1809 CCAGACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 833 GCTTAGC GC^T[ACGT]AGC
ClaI 2695 ATCGAT AT^CGAT
DsaI 2155 CCACGG C^C[GA][CT]GG
EarI 2641 CTCTTC CTCTTC(1/4)
Eco47III 146 AGCGCT AGC^GCT
EcoO65I 1263 GGTTACC G^GT[ACGT]ACC
EcoRV 1327 GATATC GAT^ATC
FauI 556 GCGGG CCCGC(4/6)
FbaI 362 TGATCA T^GATCA
FspI 1113 TGCGCA TGC^GCA
HaeII 146 AGCGCT [GA]GCGC^[CT]
HincII 2170 GTCAAC GT[CT]^[GA]AC
HindII 2170 GTCAAC GT[CT]^[GA]AC
HindIII 2815 AAGCTT A^AGCTT
Ksp632I 2641 CTCTTC CTCTTC(1/4)
MaeII 700 ACGT A^CGT
MamI 2725 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 635 CAATTG C^AATTG
MluNI 268 TGGCCA TGG^CCA
MscI 268 TGGCCA TGG^CCA
MslI 430 CATTCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 635 CAATTG C^AATTG
NaeI 1303 GCCGGC GCC^GGC
NdeI 2165 CATATG CA^TATG
NgoMIV 1303 GCCGGC G^CCGGC
PinAI 654 ACCGGT A^CCGGT
PpuMI 1374 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 2244 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2102 CTGCAG CTGCA^G
SacI 2638 GAGCTC GAGCT^C
SapI 2640 GCTCTTC GCTCTTC(1/4)
SexAI 542 ACCAGGT A^CC[AT]GGT
SmlI 172 CTTGAG C^T[CT][GA]AG
SpeI 781 ACTAGT A^CTAGT
SphI 1886 GCATGC GCATG^C
StuI 1344 AGGCCT AGG^CCT
XmnI 1895 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 154 CGCG CG^CG
2098 CGCG
AspI 100 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1361 GACTAGGTC
BglI 1298 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1303 GCCGGCCTGGC
BsaI 2450 GAGACC GGTCTC(1/5)
767 GGTCTC
BsaWI 484 ACCGGA [AT]^CCGG[AT]
654 ACCGGT
BseRI 1240 CTCCTC GAGGAG(10/8)
142 GAGGAG
BsmI 1425 GAATGC GAATGC(1/-1)
1914 GAATGC
BsrFI 654 ACCGGT [GA]^CCGG[CT]
1303 GCCGGC
BstUI 154 CGCG CG^CG
2098 CGCG
Cfr10I 654 ACCGGT [GA]^CCGG[CT]
1303 GCCGGC
DraI 721 TTTAAA TTT^AAA
1707 TTTAAA
DraII 1374 GGGACCC [GA]G^G[ACGT]CC[CT]
2140 AGGGCCC
EcoO109I 1374 GGGACCC [GA]G^G[ACGT]CC[CT]
2140 AGGGCCC
EcoRI 593 GAATTC G^AATTC
644 GAATTC
HphI 1921 GGTGA GGTGA(8/7)
2241 GGTGA
MvnI 154 CGCG CG^CG
2098 CGCG
PflFI 100 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1361 GACTAGGTC
PvuII 2117 CAGCTG CAG^CTG
2303 CAGCTG
SfcI 551 CTATAG C^T[GA][CT]AG
2102 CTGCAG
Tth111I 100 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1361 GACTAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DrdI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII