(Linear) (Six-base) MAPSORT of: KIEE2540.seq Check: 189 from: 1 to: 2922 KIEE2540.orf With 182 enzymes: SgfI * September 19, 2014 17:03 .. AccI GT'mk_AC Cuts at: 0 995 2922 Size: 995 1927 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 218 301 424 1322 1874 2628 2686 2755 Size: 218 83 123 898 552 754 58 69 Cuts at: 2755 2922 Size: 167 Fragments arranged by size: 898 754 552 218 167 123 83 69 58 AfeI AGC'GCT Cuts at: 0 148 2922 Size: 148 2774 AflIII A'CryG_T Cuts at: 0 435 2922 Size: 435 2487 AgeI A'CCGG_T Cuts at: 0 654 2922 Size: 654 2268 AlwNI CAG_nnn'CTG Cuts at: 0 2041 2119 2733 2922 Size: 2041 78 614 189 Fragments arranged by size: 2041 614 189 78 ApaI G_GGCC'C Cuts at: 0 2145 2922 Size: 2145 777 ApoI r'AATT_y Cuts at: 0 593 644 2485 2922 Size: 593 51 1841 437 Fragments arranged by size: 1841 593 437 51 AvaI C'yCGr_G Cuts at: 0 584 2922 Size: 584 2338 BamHI G'GATC_C Cuts at: 0 1797 2922 Size: 1797 1125 BanI G'GyrC_C Cuts at: 0 1006 1494 2761 2922 Size: 1006 488 1267 161 Fragments arranged by size: 1267 1006 488 161 BanII G_rGCy'C Cuts at: 0 20 2145 2380 2642 2922 Size: 20 2125 235 262 280 Fragments arranged by size: 2125 280 262 235 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 227 1045 1131 2922 Size: 227 818 86 1791 Fragments arranged by size: 1791 818 227 86 BclI T'GATC_A Cuts at: 0 362 2922 Size: 362 2560 BglI GCCn_nnn'nGGC Cuts at: 0 1304 1309 2922 Size: 1304 5 1613 Fragments arranged by size: 1613 1304 5 BlpI GC'TnA_GC Cuts at: 0 834 2922 Size: 834 2088 Bme1580I G_kGCm'C Cuts at: 0 86 1009 1497 1607 1619 2145 2922 Size: 86 923 488 110 12 526 777 Fragments arranged by size: 923 777 526 488 110 86 12 BmrI ACTGGGnnnn_n' Cuts at: 0 73 962 2662 2922 Size: 73 889 1700 260 Fragments arranged by size: 1700 889 260 73 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 985 1619 1712 2069 2292 2466 2922 Size: 985 634 93 357 223 174 456 Fragments arranged by size: 985 634 456 357 223 174 93 Bpu10I CC'TnA_GC Cuts at: 0 2510 2636 2922 Size: 2510 126 286 Fragments arranged by size: 2510 286 126 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 2922 Size: 193 2729 BsaI GGTCTCn'nnnn_ Cuts at: 0 773 2444 2922 Size: 773 1671 478 Fragments arranged by size: 1671 773 478 BsaBI GATnn'nnATC Cuts at: 0 2729 2922 Size: 2729 193 BsaWI w'CCGG_w Cuts at: 0 484 654 2922 Size: 484 170 2268 Fragments arranged by size: 2268 484 170 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 34 64 1760 1790 2922 Size: 34 30 1696 30 1132 Fragments arranged by size: 1696 1132 34 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 157 1231 2922 Size: 157 1074 1691 Fragments arranged by size: 1691 1074 157 BseYI C'CCAG_C Cuts at: 0 826 1287 1318 1477 2123 2742 2922 Size: 826 461 31 159 646 619 180 Fragments arranged by size: 826 646 619 461 180 159 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 106 2922 Size: 106 2816 BsiHKAI G_wGCw'C Cuts at: 0 859 948 2417 2642 2922 Size: 859 89 1469 225 280 Fragments arranged by size: 1469 859 280 225 89 BsmI GAATG_Cn' Cuts at: 0 1431 1920 2922 Size: 1431 489 1002 Fragments arranged by size: 1431 1002 489 Bsp1286I G_dGCh'C Cuts at: 0 20 86 859 948 1009 1497 1607 1619 Size: 20 66 773 89 61 488 110 12 Cuts at: 1619 2145 2380 2417 2642 2922 Size: 526 235 37 225 280 Fragments arranged by size: 773 526 488 280 235 225 110 89 66 61 37 20 12 BsrDI GCAATG_nn' Cuts at: 0 1109 2598 2897 2922 Size: 1109 1489 299 25 Fragments arranged by size: 1489 1109 299 25 BsrFI r'CCGG_y Cuts at: 0 654 1303 2922 Size: 654 649 1619 Fragments arranged by size: 1619 654 649 BssHII G'CGCG_C Cuts at: 0 2097 2922 Size: 2097 825 BstAPI GCAn_nnn'nTGC Cuts at: 0 2119 2922 Size: 2119 803 BstEII G'GTnAC_C Cuts at: 0 1263 2922 Size: 1263 1659 BstXI CCAn_nnnn'nTGG Cuts at: 0 1816 2922 Size: 1816 1106 BstYI r'GATC_y Cuts at: 0 306 950 1098 1797 2730 2922 Size: 306 644 148 699 933 192 Fragments arranged by size: 933 699 644 306 192 148 BtgI C'CryG_G Cuts at: 0 2155 2922 Size: 2155 767 BtsI GCAGTG_nn' Cuts at: 0 1263 2922 Size: 1263 1659 ClaI AT'CG_AT Cuts at: 0 2696 2922 Size: 2696 226 DraI TTT'AAA Cuts at: 0 723 1709 2922 Size: 723 986 1213 Fragments arranged by size: 1213 986 723 DrdI GACnn_nn'nnGTC Cuts at: 0 103 1818 2474 2922 Size: 103 1715 656 448 Fragments arranged by size: 1715 656 448 103 EaeI y'GGCC_r Cuts at: 0 2 268 2913 2922 Size: 2 266 2645 9 Fragments arranged by size: 2645 266 9 2 EarI CTCTTCn'nnn_ Cuts at: 0 2647 2922 Size: 2647 275 EcoICRI GAG'CTC Cuts at: 0 2640 2922 Size: 2640 282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 218 301 424 985 1322 1619 1712 1874 Size: 218 83 123 561 337 297 93 162 Cuts at: 1874 2069 2292 2466 2628 2686 2755 2922 Size: 195 223 174 162 58 69 167 Fragments arranged by size: 561 337 297 223 218 195 174 167 162 162 123 93 83 69 58 EcoO109I rG'GnC_Cy Cuts at: 0 1375 2141 2922 Size: 1375 766 781 Fragments arranged by size: 1375 781 766 EcoRI G'AATT_C Cuts at: 0 593 644 2922 Size: 593 51 2278 Fragments arranged by size: 2278 593 51 EcoRV GAT'ATC Cuts at: 0 1329 2922 Size: 1329 1593 FspI TGC'GCA Cuts at: 0 1115 2922 Size: 1115 1807 HaeII r_GCGC'y Cuts at: 0 150 2922 Size: 150 2772 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 34 66 479 511 1773 1805 1816 1848 Size: 34 32 413 32 1262 32 11 32 Cuts at: 1848 1926 1958 2365 2397 2922 Size: 78 32 407 32 525 Fragments arranged by size: 1262 525 413 407 78 34 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 0 2172 2922 Size: 2172 750 HindIII A'AGCT_T Cuts at: 0 2815 2922 Size: 2815 107 MfeI C'AATT_G Cuts at: 0 635 2922 Size: 635 2287 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 20 77 536 2426 2922 Size: 20 57 459 1890 496 Fragments arranged by size: 1890 496 459 57 20 MscI TGG'CCA Cuts at: 0 270 2922 Size: 270 2652 MslI CAynn'nnrTG Cuts at: 0 434 2922 Size: 434 2488 MspA1I CmG'CkG Cuts at: 0 2119 2305 2918 2922 Size: 2119 186 613 4 Fragments arranged by size: 2119 613 186 4 NaeI GCC'GGC Cuts at: 0 1305 2922 Size: 1305 1617 NdeI CA'TA_TG Cuts at: 0 2166 2922 Size: 2166 756 NgoMIV G'CCGG_C Cuts at: 0 1303 2922 Size: 1303 1619 NspI r_CATG'y Cuts at: 0 439 1316 1720 1890 2178 2922 Size: 439 877 404 170 288 744 Fragments arranged by size: 877 744 439 404 288 170 PasI CC'CwG_GG Cuts at: 0 9 2922 Size: 9 2913 PciI A'CATG_T Cuts at: 0 435 2922 Size: 435 2487 PflMI CCAn_nnn'nTGG Cuts at: 0 640 1468 1490 2922 Size: 640 828 22 1432 Fragments arranged by size: 1432 828 640 22 PfoI T'CCnGG_A Cuts at: 0 2849 2922 Size: 2849 73 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 930 962 2528 2560 2922 Size: 930 32 1566 32 362 Fragments arranged by size: 1566 930 362 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1375 2922 Size: 1375 1547 PshAI GACnn'nnGTC Cuts at: 0 2248 2922 Size: 2248 674 PspOMI G'GGCC_C Cuts at: 0 2141 2922 Size: 2141 781 PstI C_TGCA'G Cuts at: 0 2106 2922 Size: 2106 816 PvuII CAG'CTG Cuts at: 0 2119 2305 2922 Size: 2119 186 617 Fragments arranged by size: 2119 617 186 SacI G_AGCT'C Cuts at: 0 2642 2922 Size: 2642 280 SanDI GG'GwC_CC Cuts at: 0 1375 2922 Size: 1375 1547 SapI GCTCTTCn'nnn_ Cuts at: 0 2647 2922 Size: 2647 275 SexAI A'CCwGG_T Cuts at: 0 542 2922 Size: 542 2380 SfcI C'TryA_G Cuts at: 0 551 2102 2922 Size: 551 1551 820 Fragments arranged by size: 1551 820 551 SmlI C'TyrA_G Cuts at: 0 172 2922 Size: 172 2750 SpeI A'CTAG_T Cuts at: 0 781 2922 Size: 781 2141 SphI G_CATG'C Cuts at: 0 1890 2922 Size: 1890 1032 SspI AAT'ATT Cuts at: 0 462 708 1587 2922 Size: 462 246 879 1335 Fragments arranged by size: 1335 879 462 246 StuI AGG'CCT Cuts at: 0 1346 2922 Size: 1346 1576 StyI C'CwwG_G Cuts at: 0 353 1347 1949 2922 Size: 353 994 602 973 Fragments arranged by size: 994 973 602 353 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1491 1696 2468 2922 Size: 1491 205 772 454 Fragments arranged by size: 1491 772 454 205 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1760 1792 1905 1937 2922 Size: 1760 32 113 32 985 Fragments arranged by size: 1760 985 113 32 32 Tth111I GACn'n_nGTC Cuts at: 0 103 1364 2922 Size: 103 1261 1558 Fragments arranged by size: 1558 1261 103 XmnI GAAnn'nnTTC Cuts at: 0 1899 2922 Size: 1899 1023 Enzymes that do cut: AccI AcuI AfeI AflIII AgeI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BssHII BstAPI BstEII BstXI BstYI BtgI BtsI ClaI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SapI SexAI SfcI SmlI SpeI SphI SspI StuI StyI TaqII TstI Tth111I XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI BplI BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssSI BstBI BstZ17I Bsu36I BtgZI CspCI DraIII EagI EciI EcoNI FalI FseI FspAI HpaI KasI KpnI MluI NarI NcoI NheI NotI NruI NsiI PacI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI TatI XbaI XcmI XhoI XmaI ZraI