################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BalI BanII BbeI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 524 GGTACC G^GTACC AccI 658 GTAGAC GT^[AC][GT]AC AccII 1526 CGCG CG^CG Alw44I 411 GTGCAC G^TGCAC AlwNI 1382 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 411 GTGCAC G^TGCAC Asp718I 524 GGTACC G^GTACC AspHI 411 GTGCAC G[AT]GC[AT]^C BamHI 671 GGATCC G^GATCC BbvCI 792 GCTGAGG CCTCAGC(-5/-2) BglII 1197 AGATCT A^GATCT BsaBI 220 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 955 GAGACC GGTCTC(1/5) BseRI 1171 GAGGAG GAGGAG(10/8) BsgI 1078 CTGCAC GTGCAG(16/14) BsiHKAI 411 GTGCAC G[AT]GC[AT]^C BsmI 1782 GAATGC GAATGC(1/-1) BspHI 1337 TCATGA T^CATGA BspMI 651 GCAGGT ACCTGC(4/8) BsrI 1247 ACTGG ACTGG(1/-1) BstUI 1526 CGCG CG^CG BstXI 1288 CCAGAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 574 GACGTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 966 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 44 CCAAGG C^C[AT][AT]GG FauI 1662 GCGGG CCCGC(4/6) HgaI 462 GCGTC GACGC(5/10) HincII 1585 GTTGAC GT[CT]^[GA]AC HindII 1585 GTTGAC GT[CT]^[GA]AC KpnI 524 GGTACC GGTAC^C MamI 220 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1526 CGCG CG^CG RcaI 1337 TCATGA T^CATGA SapI 1369 GAAGAGC GCTCTTC(1/4) ScaI 1244 AGTACT AGT^ACT SmlI 1201 CTTGAG C^T[CT][GA]AG StyI 44 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AfaI 525 GTAC GT^AC 1245 GTAC AflIII 1833 ACACGT A^C[GA][CT]GT 1835 ACGTGT BanI 33 GGTGCC G^G[CT][GA]CC 524 GGTACC BbrPI 1721 CACGTG CAC^GTG 1834 CACGTG BclI 419 TGATCA T^GATCA 906 TGATCA BcnI 1637 CCCGG CC^[GC]GG 1694 CCCGG BfaI 805 CTAG C^TAG 1649 CTAG BmyI 411 GTGCAC G[AGT]GC[ACT]^C 622 GGGCAC BsaAI 1721 CACGTG [CT]AC^GT[GA] 1834 CACGTG BsmAI 955 GAGAC GTCTC(1/5) 609 GTCTC Bsp1286I 411 GTGCAC G[AGT]GC[ACT]^C 622 GGGCAC FbaI 419 TGATCA T^GATCA 906 TGATCA HapII 1638 CCGG C^CGG 1695 CCGG HpaII 1638 CCGG C^CGG 1695 CCGG HphI 561 GGTGA GGTGA(8/7) 1755 GGTGA MaeI 805 CTAG C^TAG 1649 CTAG MaeIII 562 GTGAC ^GT[ACGT]AC 1724 GTGAC MslI 705 CATCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 736 CACCTCCGTG MspI 1638 CCGG C^CGG 1695 CCGG NciI 1637 CCCGG CC^[GC]GG 1694 CCCGG PflMI 529 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 681 CCACCCTCTGG PmaCI 1721 CACGTG CAC^GTG 1834 CACGTG PmlI 1721 CACGTG CAC^GTG 1834 CACGTG PpuMI 505 AGGACCC [GA]G^G[AT]CC[CT] 1512 GGGACCC RsaI 525 GTAC GT^AC 1245 GTAC TfiI 941 GATTC G^A[AT]TC 1800 GAATC Tsp45I 562 GTGAC ^GT[GC]AC 1724 GTGAC Van91I 529 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 681 CCACCCTCTGG XcmI 305 CCAAAAATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 719 CCATCACTGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BbsI BbvI BpmI BpuAI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI SspI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII