(Circular) (Six-base) MAPSORT of: pFN21AE3263.seq Check: 2797 from: 1 to: 6607 pFN21AE3263 6607 bp With 182 enzymes: SgfI * March 28, 2012 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4417 278 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2664 3950 1937 Size: 727 1286 4594 Fragments arranged by size: 4594 1286 727 Acc65I G'GTAC_C Cuts at: 2529 3928 2529 Size: 1399 5208 AclI AA'CG_TT Cuts at: 1563 4734 5107 1563 Size: 3171 373 3063 Fragments arranged by size: 3171 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2411 2925 2961 3159 3227 4670 6054 6322 Size: 779 514 36 198 68 1443 1384 268 Cuts at: 6322 1632 Size: 1917 Fragments arranged by size: 1917 1443 1384 779 514 268 198 68 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6410 AflIII A'CryG_T Cuts at: 1203 3838 3840 5506 6483 1203 Size: 2635 2 1666 977 1327 Fragments arranged by size: 2635 1666 1327 977 2 AgeI A'CCGG_T Cuts at: 5438 5438 Size: 6607 AhdI GACnn_n'nnGTC Cuts at: 5336 5336 Size: 6607 AleI CACnn'nnGTG Cuts at: 1166 2745 1166 Size: 1579 5028 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6607 AlwNI CAG_nnn'CTG Cuts at: 3392 4350 5922 3392 Size: 958 1572 4077 Fragments arranged by size: 4077 1572 958 ApaLI G'TGCA_C Cuts at: 2416 4664 5820 2416 Size: 2248 1156 3203 Fragments arranged by size: 3203 2248 1156 ApoI r'AATT_y Cuts at: 1493 2159 2196 2314 3265 3462 3486 3916 4142 Size: 666 37 118 951 197 24 430 226 Cuts at: 4142 4294 6403 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 951 666 430 226 197 152 118 37 24 AseI AT'TA_AT Cuts at: 160 1032 5161 160 Size: 872 4129 1606 Fragments arranged by size: 4129 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 AvaI C'yCGr_G Cuts at: 1870 1943 3932 1870 Size: 73 1989 4545 Fragments arranged by size: 4545 1989 73 BamHI G'GATC_C Cuts at: 2676 3937 4304 2676 Size: 1261 367 4979 Fragments arranged by size: 4979 1261 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 2529 3928 5383 618 Size: 325 217 627 251 491 1399 1455 1842 Fragments arranged by size: 1842 1455 1399 627 491 325 251 217 BanII G_rGCy'C Cuts at: 1391 3926 1391 Size: 2535 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2084 3429 3678 928 Size: 971 185 1345 249 3857 Fragments arranged by size: 3857 1345 971 249 185 BbvCI CC'TCA_GC Cuts at: 2798 2798 Size: 6607 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4798 4832 1903 Size: 34 2861 34 3678 Fragments arranged by size: 3678 2861 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4499 5709 4499 Size: 1210 5397 BclI T'GATC_A Cuts at: 1548 2424 2911 1548 Size: 876 487 5244 Fragments arranged by size: 5244 876 487 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5218 136 Size: 107 122 71 631 605 3546 1525 Fragments arranged by size: 3546 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3202 6602 3202 Size: 3400 3207 BlpI GC'TnA_GC Cuts at: 4024 4024 Size: 6607 Bme1580I G_kGCm'C Cuts at: 1790 2420 2631 4668 5824 1790 Size: 630 211 2037 1156 2573 Fragments arranged by size: 2573 2037 1156 630 211 BmgBI CAC'GTC Cuts at: 1318 2581 1318 Size: 1263 5344 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3262 4338 5296 Size: 416 53 83 399 24 1845 1076 958 Cuts at: 5296 442 Size: 1753 Fragments arranged by size: 1845 1753 1076 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6607 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3732 3732 Size: 6607 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2714 3414 3512 5267 1239 Size: 1475 700 98 1755 2579 Fragments arranged by size: 2579 1755 1475 700 98 Bpu10I CC'TnA_GC Cuts at: 2798 2798 Size: 6607 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3227 4732 5597 5895 3227 Size: 1505 865 298 3939 Fragments arranged by size: 3939 1505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2954 5270 882 Size: 617 1455 2316 2219 Fragments arranged by size: 2316 2219 1455 617 BsaAI yAC'GTr Cuts at: 493 1204 3728 3841 4398 493 Size: 711 2524 113 557 2702 Fragments arranged by size: 2702 2524 711 557 113 BsaBI GATnn'nnATC Cuts at: 2229 6601 2229 Size: 4372 2235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4414 4796 5431 275 Size: 53 83 186 936 2881 382 635 1451 Fragments arranged by size: 2881 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5040 5438 5712 5859 6270 5040 Size: 398 274 147 411 5377 Fragments arranged by size: 5377 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3694 3724 1178 Size: 30 165 30 2291 30 4061 Fragments arranged by size: 4061 2291 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3191 1186 Size: 2005 4602 BseYI C'CCAG_C Cuts at: 5810 6306 5810 Size: 496 6111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3068 1163 Size: 573 1332 4702 Fragments arranged by size: 4702 1332 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4057 4818 5846 1540 Size: 275 186 2056 761 1028 2301 Fragments arranged by size: 2301 2056 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2420 4668 4753 5824 2420 Size: 2248 85 1071 3203 Fragments arranged by size: 3203 2248 1071 85 BsmI GAATG_Cn' Cuts at: 3793 4118 4211 3793 Size: 325 93 6189 Fragments arranged by size: 6189 325 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2420 2631 3926 4668 4753 5824 1391 Size: 399 630 211 1295 742 85 1071 2174 Fragments arranged by size: 2174 1295 1071 742 630 399 211 85 BspEI T'CCGG_A Cuts at: 6270 6270 Size: 6607 BspHI T'CATG_A Cuts at: 3342 4496 6226 3342 Size: 1154 1730 3723 Fragments arranged by size: 3723 1730 1154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2647 3962 844 Size: 1803 1315 3489 Fragments arranged by size: 3489 1803 1315 BsrBI CCG'CTC Cuts at: 1394 1823 4494 1394 Size: 429 2671 3507 Fragments arranged by size: 3507 2671 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5102 5276 65 Size: 1183 3854 174 1396 Fragments arranged by size: 3854 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5251 5438 1700 Size: 110 3441 187 2869 Fragments arranged by size: 3441 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6607 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6607 BssSI C'ACGA_G Cuts at: 4667 5679 4667 Size: 1012 5595 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6607 BstBI TT'CG_AA Cuts at: 5411 5411 Size: 6607 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6607 BstXI CCAn_nnnn'nTGG Cuts at: 3300 3300 Size: 6607 BstYI r'GATC_y Cuts at: 1972 2676 3202 3238 3638 3937 4304 4689 4706 Size: 704 526 36 400 299 367 385 17 Cuts at: 4706 6158 6244 6256 6602 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 704 526 400 385 367 346 299 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5116 Fragments arranged by size: 5116 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2111 487 Size: 683 56 885 4983 Fragments arranged by size: 4983 885 683 56 BtsI GCAGTG_nn' Cuts at: 2727 2789 4123 4917 4937 2727 Size: 62 1334 794 20 4397 Fragments arranged by size: 4397 1334 794 62 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6607 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6572 DraI TTT'AAA Cuts at: 3911 3911 Size: 6607 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2585 5614 809 Size: 507 1269 3029 1802 Fragments arranged by size: 3029 1802 1269 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4054 4943 6572 Size: 54 1425 50 275 18 2224 889 1629 Cuts at: 6572 8 Size: 43 Fragments arranged by size: 2224 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4054 1537 Size: 275 2242 4090 Fragments arranged by size: 4090 2242 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2822 2843 2879 3155 3185 3369 3423 3872 Size: 1469 21 36 276 30 184 54 449 Cuts at: 3872 4537 1353 Size: 665 3423 Fragments arranged by size: 3423 1469 665 449 276 184 54 36 30 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2035 3845 5194 5568 5714 387 Size: 1064 138 446 1810 1349 374 146 1280 Fragments arranged by size: 1810 1349 1280 1064 446 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2411 2714 2925 2961 3159 3227 3414 Size: 393 779 303 211 36 198 68 187 Cuts at: 3414 3512 4670 5267 6054 6322 1239 Size: 98 1158 597 787 268 1524 Fragments arranged by size: 1524 1158 787 779 597 393 303 268 211 198 187 98 68 36 EcoNI CCTnn'n_nnAGG Cuts at: 2975 2975 Size: 6607 EcoO109I rG'GnC_Cy Cuts at: 2511 3123 3518 2511 Size: 612 395 5600 Fragments arranged by size: 5600 612 395 EcoRI G'AATT_C Cuts at: 3916 4294 3916 Size: 378 6229 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2569 2569 Size: 6607 FspI TGC'GCA Cuts at: 5113 5113 Size: 6607 HaeII r_GCGC'y Cuts at: 1678 5754 1678 Size: 4076 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2921 2953 3395 3427 3565 Size: 32 135 32 1349 32 442 32 138 Cuts at: 3565 3597 5248 5280 5322 5354 1373 Size: 32 1651 32 42 32 2626 Fragments arranged by size: 2626 1651 1349 442 138 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3592 3951 4197 669 Size: 988 281 1654 359 246 3079 Fragments arranged by size: 3079 1654 988 359 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6607 HpaI GTT'AAC Cuts at: 4197 4197 Size: 6607 KpnI G_GTAC'C Cuts at: 2533 3932 2533 Size: 1399 5208 MfeI C'AATT_G Cuts at: 4206 4206 Size: 6607 MluI A'CGCG_T Cuts at: 6483 6483 Size: 6607 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2120 3469 5721 5905 1305 Size: 815 1349 2252 184 2007 Fragments arranged by size: 2252 2007 1349 815 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4785 Fragments arranged by size: 4785 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2714 2745 4565 4924 5083 518 Size: 648 1548 31 1820 359 159 2042 Fragments arranged by size: 2042 1820 1548 648 359 159 31 MspA1I CmG'CkG Cuts at: 1606 2379 2389 3389 3451 4023 4700 5848 6093 Size: 773 10 1000 62 572 677 1148 245 Cuts at: 6093 1606 Size: 2120 Fragments arranged by size: 2120 1148 1000 773 677 572 245 62 10 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5116 Fragments arranged by size: 5116 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6607 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6607 NotI GC'GGCC_GC Cuts at: 4054 4054 Size: 6607 NspI r_CATG'y Cuts at: 1132 1229 3965 5510 1132 Size: 97 2736 1545 2229 Fragments arranged by size: 2736 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2404 1348 Size: 1056 5551 PciI A'CATG_T Cuts at: 5506 5506 Size: 6607 PflMI CCAn_nnn'nTGG Cuts at: 1108 2540 2692 1108 Size: 1432 152 5023 Fragments arranged by size: 5023 1432 152 PfoI T'CCnGG_A Cuts at: 2679 3182 3641 2679 Size: 503 459 5645 Fragments arranged by size: 5645 503 459 PmeI GTTT'AAAC Cuts at: 3911 3911 Size: 6607 PmlI CAC'GTG Cuts at: 3728 3841 3728 Size: 113 6494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4676 4708 6189 6221 4676 Size: 32 1481 32 5062 Fragments arranged by size: 5062 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2511 3518 2511 Size: 1007 5600 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6607 PsiI TTA'TAA Cuts at: 2640 4177 2640 Size: 1537 5070 PstI C_TGCA'G Cuts at: 830 3959 830 Size: 3129 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6607 PvuII CAG'CTG Cuts at: 2389 3389 3451 2389 Size: 1000 62 5545 Fragments arranged by size: 5545 1000 62 SalI G'TCGA_C Cuts at: 1936 3949 1936 Size: 2013 4594 SanDI GG'GwC_CC Cuts at: 3518 3518 Size: 6607 SapI GCTCTTCn'nnn_ Cuts at: 3369 3369 Size: 6607 SbfI CC_TGCA'GG Cuts at: 3959 3959 Size: 6607 ScaI AGT'ACT Cuts at: 3251 4855 6567 3251 Size: 1604 1712 3291 Fragments arranged by size: 3291 1712 1604 SfcI C'TryA_G Cuts at: 826 1045 3955 5090 5771 5962 826 Size: 219 2910 1135 681 191 1471 Fragments arranged by size: 2910 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6607 SmaI CCC'GGG Cuts at: 1872 3934 1872 Size: 2062 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3206 4711 5612 5874 820 Size: 197 926 1263 1505 901 262 1553 Fragments arranged by size: 1553 1505 1263 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4398 493 Size: 3905 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6607 SphI G_CATG'C Cuts at: 1132 3965 1132 Size: 2833 3774 SspI AAT'ATT Cuts at: 5 52 2257 2305 2922 4531 5 Size: 47 2205 48 617 1609 2081 Fragments arranged by size: 2205 2081 1609 617 48 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6607 StyI C'CwwG_G Cuts at: 513 1065 2004 2049 4046 513 Size: 552 939 45 1997 3074 Fragments arranged by size: 3074 1997 939 552 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4634 4651 4804 4989 5434 325 Size: 1255 3054 17 153 185 445 1498 Fragments arranged by size: 3054 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3249 4853 6565 Size: 274 80 33 51 1443 1272 1604 1712 Cuts at: 6565 96 Size: 138 Fragments arranged by size: 1712 1604 1443 1272 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3045 3077 6567 6599 3045 Size: 32 3490 32 3053 Fragments arranged by size: 3490 3053 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6607 XbaI T'CTAG_A Cuts at: 3943 3943 Size: 6607 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2317 2731 2317 Size: 414 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6607 XmaI C'CCGG_G Cuts at: 1870 3932 1870 Size: 2062 4545 XmnI GAAnn'nnTTC Cuts at: 4736 4736 Size: 6607 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4415 276 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI