################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 25 GAATTC [GA]^AATT[CT] AfaI 171 GTAC GT^AC AflIII 479 ACATGT A^C[GA][CT]GT AocI 770 CCTTAGG CC^T[ACGT]AGG Aor51HI 105 AGCGCT AGC^GCT ApoI 25 GAATTC [GA]^AATT[CT] Asp700I 674 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 686 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 775 GGACC G^G[AT]CC BcnI 77 CCGGG CC^[GC]GG BsgI 70 CTGCAC GTGCAG(16/14) BsiYI 30 CCTGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 30 CCTGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 900 GAGAC GTCTC(1/5) BspHI 632 TCATGA T^CATGA BspLU11I 479 ACATGT A^CATGT BsrDI 129 CATTGC GCAATG(2/0) BstAPI 63 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 770 CCTTAGG CC^T[ACGT]AGG CfoI 106 GCGC GCG^C Eco47III 105 AGCGCT AGC^GCT Eco81I 770 CCTTAGG CC^T[ACGT]AGG EcoRI 25 GAATTC G^AATTC HaeII 105 AGCGCT [GA]GCGC^[CT] HapII 77 CCGG C^CGG HhaI 106 GCGC GCG^C HinP1I 106 GCGC G^CGC HpaII 77 CCGG C^CGG HphI 431 TCACC GGTGA(8/7) MaeII 494 ACGT A^CGT MfeI 779 CAATTG C^AATTG MseI 884 TTAA T^TAA MspI 77 CCGG C^CGG MunI 779 CAATTG C^AATTG NciI 77 CCGGG CC^[GC]GG NlaIV 116 GGAACC GG[ACGT]^[ACGT]CC NspI 479 ACATGT [GA]CATG^[CT] PflFI 686 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 61 CTGCAG CTGCA^G RcaI 632 TCATGA T^CATGA RsaI 171 GTAC GT^AC SacI 600 GAGCTC GAGCT^C SfcI 61 CTGCAG C^T[GA][CT]AG SmlI 290 CTCAAG C^T[CT][GA]AG TaqI 693 TCGA T^CGA TfiI 674 GAATC G^A[AT]TC Tru9I 884 TTAA T^TAA Tth111I 686 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 693 TCGA T^CGA XmnI 674 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 357 CCGC CCGC(-3/-1) 133 GCGG AlwNI 64 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 218 CAGTCACTG BanII 600 GAGCTC G[GA]GC[CT]^C 757 GGGCTC BlpI 322 GCTGAGC GC^T[ACGT]AGC 441 GCTAAGC Bpu1102I 322 GCTGAGC GC^T[ACGT]AGC 441 GCTAAGC BsaJI 551 CCCTGG C^C[ACGT][ACGT]GG 590 CCCTGG BsrI 472 ACTGG ACTGG(1/-1) 519 CCAGT BstYI 201 GGATCT [GA]^GATC[CT] 502 AGATCC Cac8I 72 GCACGC GC[ACGT]^[ACGT]GC 327 GCTGGC CelII 322 GCTGAGC GC^T[ACGT]AGC 441 GCTAAGC MflI 201 GGATCT [GA]^GATC[CT] 502 AGATCC MslI 12 CACAAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 162 CACACCCATG Tsp509I 26 AATT ^AATT 780 AATT TspRI 222 CACTG CA[GC]TG[ACGT][ACGT]^ 471 CACTG XhoII 201 GGATCT [GA]^GATC[CT] 502 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AlwI AspHI BbvI BmyI BsiHKAI Bsp1286I BspMI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII PleI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I