Restriction map for
pFN21AE6042
(Linear) (Six-base) MAPSORT of: KIEE6042.seq Check: 2984 from: 1 to: 951
KIEE6042.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 951
Size: 727 224
AccI GT'mk_AC
Cuts at: 0 248 951
Size: 248 703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 951
Size: 574 377
AleI CACnn'nnGTG
Cuts at: 0 229 775 951
Size: 229 546 176
Fragments arranged by size:
546 229 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 403 951
Size: 403 548
AlwNI CAG_nnn'CTG
Cuts at: 0 328 951
Size: 328 623
ApaI G_GGCC'C
Cuts at: 0 900 951
Size: 900 51
ApoI r'AATT_y
Cuts at: 0 659 951
Size: 659 292
AvaI C'yCGr_G
Cuts at: 0 512 642 951
Size: 512 130 309
Fragments arranged by size:
512 309 130
AvrII C'CTAG_G
Cuts at: 0 342 951
Size: 342 609
BanI G'GyrC_C
Cuts at: 0 540 951
Size: 540 411
BanII G_rGCy'C
Cuts at: 0 649 900 951
Size: 649 251 51
Fragments arranged by size:
649 251 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 526 560 951
Size: 526 34 391
Fragments arranged by size:
526 391 34
BglII A'GATC_T
Cuts at: 0 416 951
Size: 416 535
BlpI GC'TnA_GC
Cuts at: 0 187 951
Size: 187 764
Bme1580I G_kGCm'C
Cuts at: 0 236 900 951
Size: 236 664 51
Fragments arranged by size:
664 236 51
Bpu10I CC'TnA_GC
Cuts at: 0 318 803 951
Size: 318 485 148
Fragments arranged by size:
485 318 148
BsaAI yAC'GTr
Cuts at: 0 369 777 951
Size: 369 408 174
Fragments arranged by size:
408 369 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 951
Size: 139 30 782
Fragments arranged by size:
782 139 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 714 951
Size: 610 104 237
Fragments arranged by size:
610 237 104
BseYI C'CCAG_C
Cuts at: 0 447 951
Size: 447 504
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 549 951
Size: 373 176 402
Fragments arranged by size:
402 373 176
Bsp1286I G_dGCh'C
Cuts at: 0 236 649 900 951
Size: 236 413 251 51
Fragments arranged by size:
413 251 236 51
BspHI T'CATG_A
Cuts at: 0 404 951
Size: 404 547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 951
Size: 727 224
BsrGI T'GTAC_A
Cuts at: 0 767 951
Size: 767 184
BstAPI GCAn_nnn'nTGC
Cuts at: 0 419 951
Size: 419 532
BstEII G'GTnAC_C
Cuts at: 0 939 951
Size: 939 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 243 951
Size: 126 117 708
Fragments arranged by size:
708 126 117
BstYI r'GATC_y
Cuts at: 0 416 951
Size: 416 535
Bsu36I CC'TnA_GG
Cuts at: 0 202 874 930 951
Size: 202 672 56 21
Fragments arranged by size:
672 202 56 21
BtsI GCAGTG_nn'
Cuts at: 0 290 951
Size: 290 661
ClaI AT'CG_AT
Cuts at: 0 461 951
Size: 461 490
DraIII CAC_nnn'GTG
Cuts at: 0 735 951
Size: 735 216
EaeI y'GGCC_r
Cuts at: 0 713 951
Size: 713 238
EarI CTCTTCn'nnn_
Cuts at: 0 754 951
Size: 754 197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 951
Size: 574 377
EcoNI CCTnn'n_nnAGG
Cuts at: 0 904 951
Size: 904 47
EcoO109I rG'GnC_Cy
Cuts at: 0 896 897 951
Size: 896 1 54
Fragments arranged by size:
896 54 1
EcoRI G'AATT_C
Cuts at: 0 659 951
Size: 659 292
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 443 475 951
Size: 443 32 476
Fragments arranged by size:
476 443 32
HincII GTy'rAC
Cuts at: 0 249 951
Size: 249 702
MscI TGG'CCA
Cuts at: 0 715 951
Size: 715 236
MslI CAynn'nnrTG
Cuts at: 0 172 229 775 847 951
Size: 172 57 546 72 104
Fragments arranged by size:
546 172 104 72 57
PshAI GACnn'nnGTC
Cuts at: 0 351 951
Size: 351 600
PspOMI G'GGCC_C
Cuts at: 0 896 951
Size: 896 55
SalI G'TCGA_C
Cuts at: 0 247 951
Size: 247 704
SexAI A'CCwGG_T
Cuts at: 0 251 951
Size: 251 700
SfcI C'TryA_G
Cuts at: 0 901 951
Size: 901 50
SnaBI TAC'GTA
Cuts at: 0 369 951
Size: 369 582
StyI C'CwwG_G
Cuts at: 0 342 951
Size: 342 609
TatI w'GTAC_w
Cuts at: 0 176 767 951
Size: 176 591 184
Fragments arranged by size:
591 184 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 139 171 951
Size: 139 32 780
Fragments arranged by size:
780 139 32
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BcgI BglII BlpI Bme1580I
Bpu10I BsaAI BsaXI BseRI BseYI BsgI Bsp1286I BspHI
BspMI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtsI
ClaI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
Hin4I HincII MscI MslI PshAI PspOMI SalI SexAI
SfcI SnaBI StyI TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstZ17I BtgI BtgZI CspCI
DraI DrdI EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI