Restriction map for
pFN21AE6032
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# NO CUT ENZYMES ( 167 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI HinP1I HindIII KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 877 GACGTC GACGT^C
AcsI 114 AAATTT [GA]^AATT[CT]
AcyI 877 GACGTC G[GA]^CG[CT]C
AflIII 563 ACACGT A^C[GA][CT]GT
AluI 185 AGCT AG^CT
ApoI 114 AAATTT [GA]^AATT[CT]
AvaI 793 CCCGAG C^[CT]CG[GA]G
BanI 430 GGTGCC G^G[CT][GA]CC
BanII 444 GGGCTC G[GA]GC[CT]^C
BcnI 62 CCGGG CC^[GC]GG
BglII 290 AGATCT A^GATCT
BmyI 444 GGGCTC G[AGT]GC[ACT]^C
BsaBI 585 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 877 GACGTC G[GA]^CG[CT]C
BsgI 557 GTGCAG GTGCAG(16/14)
BsoBI 793 CCCGAG C^[CT]CG[GA]G
Bsp1286I 444 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 587 TGTACA T^GTACA
BspHI 122 TCATGA T^CATGA
BsrGI 587 TGTACA T^GTACA
DraI 892 TTTAAA TTT^AAA
HapII 62 CCGG C^CGG
Hin1I 877 GACGTC G[GA]^CG[CT]C
HincII 657 GTTAAC GT[CT]^[GA]AC
HindII 657 GTTAAC GT[CT]^[GA]AC
HpaI 657 GTTAAC GTT^AAC
HpaII 62 CCGG C^CGG
MamI 585 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 184 CAGCTG C[AC]G^C[GT]G
MspI 62 CCGG C^CGG
NciI 62 CCGGG CC^[GC]GG
PleI 145 GACTC GAGTC(4/5)
PvuII 184 CAGCTG CAG^CTG
RcaI 122 TCATGA T^CATGA
SphI 671 GCATGC GCATG^C
SspBI 587 TGTACA T^GTACA
StuI 698 AGGCCT AGG^CCT
TaqI 452 TCGA T^CGA
TfiI 135 GATTC G^A[AT]TC
Tsp509I 115 AATT ^AATT
TthHB8I 452 TCGA T^CGA
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 81 GATCC GGATC(4/5)
531 GATCC
BsaAI 358 TACGTG [CT]AC^GT[GA]
766 TACGTG
BsaJI 33 CCTGGG C^C[ACGT][ACGT]GG
333 CCTGGG
BsmFI 223 GTCCC GGGAC(10/14)
487 GTCCC
BstYI 290 AGATCT [GA]^GATC[CT]
530 AGATCC
Cac8I 420 GCTGGC GC[ACGT]^[ACGT]GC
671 GCATGC
Cfr13I 101 GGGCC G^G[ACGT]CC
336 GGGCC
HinfI 135 GATTC G^A[ACGT]TC
145 GACTC
MflI 290 AGATCT [GA]^GATC[CT]
530 AGATCC
NspI 671 GCATGC [GA]CATG^[CT]
907 GCATGT
Sau96I 101 GGGCC G^G[ACGT]CC
336 GGGCC
SfcI 728 CTGTAG C^T[GA][CT]AG
858 CTACAG
Tsp45I 106 GTGAC ^GT[GC]AC
832 GTCAC
XhoII 290 AGATCT [GA]^GATC[CT]
530 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AfaI BbvI BsiYI BslI
BsrI BssKI BstF5I BstNI DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SfaNI Tru9I TseI
TspRI