################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AlwNI 16 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 400 GTGCTC G[AT]GC[AT]^C BanI 292 GGCACC G^G[CT][GA]CC BanII 453 GGGCTC G[GA]GC[CT]^C BfaI 343 CTAG C^TAG BglI 346 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 59 AGATCT A^GATCT BlpI 912 GCTGAGC GC^T[ACGT]AGC Bpu1102I 912 GCTGAGC GC^T[ACGT]AGC BsiHKAI 400 GTGCTC G[AT]GC[AT]^C BsmAI 422 GTCTC GTCTC(1/5) BspHI 404 TCATGA T^CATGA BstXI 236 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 912 GCTGAGC GC^T[ACGT]AGC DraII 539 AGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 350 CCATGG C^C[GA][CT]GG EarI 748 CTCTTC CTCTTC(1/4) EcoO109I 539 AGGTCCC [GA]G^G[ACGT]CC[CT] Ksp632I 748 CTCTTC CTCTTC(1/4) MaeI 343 CTAG C^TAG MspA1I 253 CAGCTG C[AC]G^C[GT]G NcoI 350 CCATGG C^CATGG NspI 776 ACATGC [GA]CATG^[CT] PflMI 15 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 154 GACTC GAGTC(4/5) PpuMI 539 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 253 CAGCTG CAG^CTG RcaI 404 TCATGA T^CATGA SfaNI 433 GCATC GCATC(5/9) SspI 124 AATATT AAT^ATT StuI 707 AGGCCT AGG^CCT Van91I 15 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 144 GATCC GGATC(4/5) 509 GATCC AvaII 279 GGTCC G^G[AT]CC 540 GGTCC BalI 8 TGGCCA TGG^CCA 257 TGGCCA BmyI 400 GTGCTC G[AGT]GC[ACT]^C 453 GGGCTC BsgI 326 GTGCAG GTGCAG(16/14) 919 GTGCAG BsmFI 232 GTCCC GGGAC(10/14) 541 GTCCC Bsp1286I 400 GTGCTC G[AGT]GC[ACT]^C 453 GGGCTC BspMI 311 ACCTGC ACCTGC(4/8) 718 ACCTGC BstYI 59 AGATCT [GA]^GATC[CT] 508 AGATCC Cfr13I 279 GGTCC G^G[ACGT]CC 540 GGTCC EcoT14I 282 CCTTGG C^C[AT][AT]GG 350 CCATGG MflI 59 AGATCT [GA]^GATC[CT] 508 AGATCC MluNI 8 TGGCCA TGG^CCA 257 TGGCCA MscI 8 TGGCCA TGG^CCA 257 TGGCCA NlaIV 292 GGCACC GG[ACGT]^[ACGT]CC 540 GGTCCC PstI 313 CTGCAG CTGCA^G 504 CTGCAG Sau96I 279 GGTCC G^G[ACGT]CC 540 GGTCC SfcI 313 CTGCAG C^T[GA][CT]AG 504 CTGCAG StyI 282 CCTTGG C^C[AT][AT]GG 350 CCATGG XcmI 11 CCAACCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 15 CCAGACCCTGGGTGG XhoII 59 AGATCT [GA]^GATC[CT] 508 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AfaI AluI BbvI BsaJI BseRI BsiYI BslI BssKI BstF5I BstNI Cac8I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI TfiI TseI Tsp45I TspRI