################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaBI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HindIII KasI KpnI KspI MamI MluI MroI MspA1I MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 778 GTCTAC GT^[AC][GT]AC AciI 375 CCGC CCGC(-3/-1) AcsI 343 GAATTT [GA]^AATT[CT] AcyI 166 GACGCC G[GA]^CG[CT]C ApoI 343 GAATTT [GA]^AATT[CT] AspI 262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 261 GGACC G^G[AT]CC BbsI 838 GTCTTC GAAGAC(2/6) BglI 358 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 838 GTCTTC GAAGAC(2/6) BsaAI 379 TACGTG [CT]AC^GT[GA] BsaHI 166 GACGCC G[GA]^CG[CT]C BsaWI 858 TCCGGT [AT]^CCGG[AT] BspHI 416 TCATGA T^CATGA BsrI 31 ACTGG ACTGG(1/-1) BstYI 520 AGATCC [GA]^GATC[CT] DsaI 362 CCATGG C^C[GA][CT]GG EcoNI 258 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 362 CCATGG C^C[AT][AT]GG HgaI 166 GACGC GACGC(5/10) Hin1I 166 GACGCC G[GA]^CG[CT]C HincII 924 GTTAAC GT[CT]^[GA]AC HindII 924 GTTAAC GT[CT]^[GA]AC HpaI 924 GTTAAC GTT^AAC MaeII 380 ACGT A^CGT MfeI 205 CAATTG C^AATTG MflI 520 AGATCC [GA]^GATC[CT] MunI 205 CAATTG C^AATTG NcoI 362 CCATGG C^CATGG NlaIV 458 GGTTCC GG[ACGT]^[ACGT]CC NspI 788 ACATGC [GA]CATG^[CT] PflFI 262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 264 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 59 GACTC GAGTC(4/5) PshAI 747 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 516 CTGCAG CTGCA^G RcaI 416 TCATGA T^CATGA SexAI 263 ACCAGGT A^CC[AT]GGT StuI 719 AGGCCT AGG^CCT StyI 362 CCATGG C^C[AT][AT]GG Tth111I 262 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 264 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 248 CCAAGATGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 520 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 290 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 519 CAGATCCTG BanII 465 GGGCTC G[GA]GC[CT]^C 908 GGGCTC BbvI 452 GCTGC GCAGC(8/12) 772 GCTGC BfaI 331 CTAG C^TAG 355 CTAG BsaI 598 GAGACC GGTCTC(1/5) 285 GGTCTC BsmFI 57 GGGAC GGGAC(10/14) 553 GTCCC BspMI 730 ACCTGC ACCTGC(4/8) 334 GCAGGT Cfr13I 261 GGACC G^G[ACGT]CC 708 GGGCC EarI 61 CTCTTC CTCTTC(1/4) 760 CTCTTC Fnu4HI 452 GCTGC GC^[ACGT]GC 772 GCTGC HapII 711 CCGG C^CGG 859 CCGG HpaII 711 CCGG C^CGG 859 CCGG ItaI 452 GCTGC GC^[ACGT]GC 772 GCTGC Ksp632I 61 CTCTTC CTCTTC(1/4) 760 CTCTTC MaeI 331 CTAG C^TAG 355 CTAG MseI 874 TTAA T^TAA 925 TTAA MspI 711 CCGG C^CGG 859 CCGG MwoI 246 GCCCAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 358 GCCACCATGGC Sau96I 261 GGACC G^G[ACGT]CC 708 GGGCC SfcI 516 CTGCAG C^T[GA][CT]AG 879 CTATAG TfiI 531 GATTC G^A[AT]TC 935 GATTC Tru9I 874 TTAA T^TAA 925 TTAA TseI 452 GCTGC G^C[AT]GC 772 GCTGC Tsp509I 206 AATT ^AATT 344 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AfaI AluI AlwI BalI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI DdeI DpnII EaeI EcoRII FokI HaeIII HinfI HphI MaeIII MboI MboII MluNI MnlI MscI MslI MvaI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI Tsp45I TspRI