(Circular) (Six-base) MAPSORT of: pFN21AE6022.seq Check: 7535 from: 1 to: 5650 pFN21AE6022 5650 bp With 182 enzymes: SgfI * December 8, 2014 13:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2744 2744 Size: 5650 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2784 2993 1937 Size: 847 209 4594 Fragments arranged by size: 4594 847 209 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2053 2144 2939 3713 5097 5365 1632 Size: 421 91 795 774 1384 268 1917 Fragments arranged by size: 1917 1384 795 774 421 268 91 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 4379 4379 Size: 5650 AleI CACnn'nnGTG Cuts at: 1166 2864 1166 Size: 1698 3952 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2473 1184 Size: 1289 4361 AlwNI CAG_nnn'CTG Cuts at: 2300 2529 3393 4965 2300 Size: 229 864 1572 2985 Fragments arranged by size: 2985 1572 864 229 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2348 2959 3185 3337 5446 1493 Size: 855 611 226 152 2109 1697 Fragments arranged by size: 2109 1697 855 611 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2975 1870 Size: 73 1032 4545 Fragments arranged by size: 4545 1032 73 BamHI G'GATC_C Cuts at: 2980 3347 2980 Size: 367 5283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2971 4426 618 Size: 325 217 627 1184 1455 1842 Fragments arranged by size: 1842 1455 1184 627 325 217 BanII G_rGCy'C Cuts at: 1391 2474 2917 2969 1391 Size: 1083 443 52 4072 Fragments arranged by size: 4072 1083 443 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2836 928 Size: 971 937 3742 Fragments arranged by size: 3742 971 937 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 1548 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2369 4261 136 Size: 107 122 71 631 605 697 1892 1525 Fragments arranged by size: 1892 1525 697 631 605 122 107 71 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 2253 3711 4867 1790 Size: 463 1458 1156 2573 Fragments arranged by size: 2573 1458 1156 463 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3381 4339 442 Size: 416 53 83 399 24 1964 958 1753 Fragments arranged by size: 1964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4310 1239 Size: 3071 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3775 4640 4938 3775 Size: 865 298 4487 Fragments arranged by size: 4487 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2296 2597 4313 882 Size: 617 797 301 1716 2219 Fragments arranged by size: 2219 1716 797 617 301 BsaAI yAC'GTr Cuts at: 493 1204 2386 3441 493 Size: 711 1182 1055 2702 Fragments arranged by size: 2702 1182 1055 711 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2172 3457 3839 4474 Size: 53 83 186 936 639 1285 382 635 Cuts at: 4474 275 Size: 1451 Fragments arranged by size: 1451 1285 936 639 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2863 4083 4481 4755 4902 5313 2863 Size: 1220 398 274 147 411 3200 Fragments arranged by size: 3200 1220 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2664 2694 1178 Size: 30 165 30 1261 30 4134 Fragments arranged by size: 4134 1261 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2627 2672 2731 1186 Size: 1441 45 59 4105 Fragments arranged by size: 4105 1441 59 45 BseYI C'CCAG_C Cuts at: 2801 4853 5349 2801 Size: 2052 496 3102 Fragments arranged by size: 3102 2052 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5077 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3861 4889 1540 Size: 275 186 1099 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3711 3796 4867 3711 Size: 85 1071 4494 Fragments arranged by size: 4494 1071 85 BsmI GAATG_Cn' Cuts at: 3161 3254 3161 Size: 93 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2474 2917 2969 3711 3796 4867 Size: 399 463 221 443 52 742 85 1071 Cuts at: 4867 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 463 443 399 221 85 52 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 2421 3539 5269 2421 Size: 1118 1730 2802 Fragments arranged by size: 2802 1730 1118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2330 2744 3005 844 Size: 1486 414 261 3489 Fragments arranged by size: 3489 1486 414 261 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4145 4319 65 Size: 1183 2897 174 1396 Fragments arranged by size: 2897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4294 4481 1700 Size: 110 2484 187 2869 Fragments arranged by size: 2869 2484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5650 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 3710 4722 3710 Size: 1012 4638 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5650 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5650 BstYI r'GATC_y Cuts at: 1972 2525 2980 3347 3732 3749 5201 5287 5299 Size: 553 455 367 385 17 1452 86 12 Cuts at: 5299 5645 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 553 455 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2367 513 Size: 552 939 363 3796 Fragments arranged by size: 3796 939 552 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4911 Fragments arranged by size: 4911 683 56 BtsI GCAGTG_nn' Cuts at: 3166 3960 3980 3166 Size: 794 20 4836 Fragments arranged by size: 4836 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4657 809 Size: 507 3341 1802 Fragments arranged by size: 3341 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2025 2388 Size: 54 1425 50 275 18 177 18 363 Cuts at: 2388 3097 3986 5615 8 Size: 709 889 1629 43 Fragments arranged by size: 1629 1425 889 709 363 275 177 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2771 3580 1353 Size: 719 699 809 3423 Fragments arranged by size: 3423 809 719 699 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4237 4611 4757 387 Size: 1064 138 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2144 2939 3713 4310 5097 5365 Size: 393 421 91 795 774 597 787 268 Cuts at: 5365 1239 Size: 1524 Fragments arranged by size: 1524 795 787 774 597 421 393 268 91 EcoNI CCTnn'n_nnAGG Cuts at: 2267 2267 Size: 5650 EcoRI G'AATT_C Cuts at: 2959 3337 2959 Size: 378 5272 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 4797 1678 Size: 3119 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4291 4323 4365 4397 1373 Size: 32 135 32 2719 32 42 32 2626 Fragments arranged by size: 2719 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2931 2994 3240 669 Size: 988 281 993 63 246 3079 Fragments arranged by size: 3079 993 988 281 246 63 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 2931 3240 2931 Size: 309 5341 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 2210 3249 2210 Size: 1039 4611 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2025 4764 4948 1305 Size: 720 2739 184 2007 Fragments arranged by size: 2739 2007 720 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2027 2390 10 Size: 54 1425 343 177 18 363 3270 Fragments arranged by size: 3270 1425 363 343 177 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2021 2057 2111 2225 2279 2504 2645 Size: 648 855 36 54 114 54 225 141 Cuts at: 2645 2792 2864 3608 3967 4126 518 Size: 147 72 744 359 159 2042 Fragments arranged by size: 2042 855 744 648 359 225 159 147 141 114 72 54 54 36 MspA1I CmG'CkG Cuts at: 1606 3066 3743 4891 5136 1606 Size: 1460 677 1148 245 2120 Fragments arranged by size: 2120 1460 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2367 513 Size: 552 939 363 3796 Fragments arranged by size: 3796 939 552 363 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 2797 3008 4553 1132 Size: 97 1568 211 1545 2229 Fragments arranged by size: 2229 1568 1545 211 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5650 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 2275 1108 Size: 1167 4483 PfoI T'CCnGG_A Cuts at: 2262 2262 Size: 5650 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2756 1613 Size: 1143 4507 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 2525 3002 830 Size: 1695 477 3478 Fragments arranged by size: 3478 1695 477 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 3898 5610 3898 Size: 1712 3938 SexAI A'CCwGG_T Cuts at: 2268 2268 Size: 5650 SfcI C'TryA_G Cuts at: 826 1045 2521 2884 2998 4133 4814 5005 826 Size: 219 1476 363 114 1135 681 191 1471 Fragments arranged by size: 1476 1471 1135 681 363 219 191 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2977 1872 Size: 1105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3754 4655 4917 820 Size: 197 926 1811 901 262 1553 Fragments arranged by size: 1811 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 2726 1517 Size: 1209 4441 StyI C'CwwG_G Cuts at: 513 1065 2004 2367 3089 513 Size: 552 939 363 722 3074 Fragments arranged by size: 3074 939 722 552 363 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2267 3677 3694 3847 4032 4477 325 Size: 1255 687 1410 17 153 185 445 1498 Fragments arranged by size: 1498 1410 1255 687 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2193 3896 5608 Size: 274 80 33 51 1443 216 1703 1712 Cuts at: 5608 96 Size: 138 Fragments arranged by size: 1712 1703 1443 274 216 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5610 5642 5610 Size: 32 5618 Tth111I GACn'n_nGTC Cuts at: 1316 2270 1316 Size: 954 4696 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2260 2260 Size: 5650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2975 1870 Size: 1105 4545 XmnI GAAnn'nnTTC Cuts at: 3779 3779 Size: 5650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI