Restriction map for
pFN21AE6022
(Linear) (Six-base) MAPSORT of: KIEE6022.seq Check: 7394 from: 1 to: 945
KIEE6022.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 739 945
Size: 739 206
AccI GT'mk_AC
Cuts at: 0 779 945
Size: 779 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 139 934 945
Size: 48 91 795 11
Fragments arranged by size:
795 91 48 11
AleI CACnn'nnGTG
Cuts at: 0 859 945
Size: 859 86
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 468 945
Size: 468 477
AlwNI CAG_nnn'CTG
Cuts at: 0 295 524 945
Size: 295 229 421
Fragments arranged by size:
421 295 229
ApoI r'AATT_y
Cuts at: 0 343 945
Size: 343 602
BanII G_rGCy'C
Cuts at: 0 469 912 945
Size: 469 443 33
Fragments arranged by size:
469 443 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 831 945
Size: 831 114
BglI GCCn_nnn'nGGC
Cuts at: 0 364 945
Size: 364 581
Bme1580I G_kGCm'C
Cuts at: 0 248 945
Size: 248 697
BsaI GGTCTCn'nnnn_
Cuts at: 0 291 592 945
Size: 291 301 353
Fragments arranged by size:
353 301 291
BsaAI yAC'GTr
Cuts at: 0 381 945
Size: 381 564
BsaHI Gr'CG_yC
Cuts at: 0 167 945
Size: 167 778
BsaWI w'CCGG_w
Cuts at: 0 858 945
Size: 858 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 659 689 945
Size: 659 30 256
Fragments arranged by size:
659 256 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 622 667 726 945
Size: 622 45 59 219
Fragments arranged by size:
622 219 59 45
BseYI C'CCAG_C
Cuts at: 0 796 945
Size: 796 149
Bsp1286I G_dGCh'C
Cuts at: 0 248 469 912 945
Size: 248 221 443 33
Fragments arranged by size:
443 248 221 33
BspHI T'CATG_A
Cuts at: 0 416 945
Size: 416 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 325 739 945
Size: 325 414 206
Fragments arranged by size:
414 325 206
BstYI r'GATC_y
Cuts at: 0 520 945
Size: 520 425
BtgI C'CryG_G
Cuts at: 0 362 945
Size: 362 583
EaeI y'GGCC_r
Cuts at: 0 2 20 383 945
Size: 2 18 363 562
Fragments arranged by size:
562 363 18 2
EarI CTCTTCn'nnn_
Cuts at: 0 67 766 945
Size: 67 699 179
Fragments arranged by size:
699 179 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 139 934 945
Size: 48 91 795 11
Fragments arranged by size:
795 91 48 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 945
Size: 262 683
HincII GTy'rAC
Cuts at: 0 926 945
Size: 926 19
HpaI GTT'AAC
Cuts at: 0 926 945
Size: 926 19
MfeI C'AATT_G
Cuts at: 0 205 945
Size: 205 740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 945
Size: 20 925
MscI TGG'CCA
Cuts at: 0 4 22 385 945
Size: 4 18 363 560
Fragments arranged by size:
560 363 18 4
MslI CAynn'nnrTG
Cuts at: 0 16 52 106 220 274 499 640 787
Size: 16 36 54 114 54 225 141 147
Cuts at: 787 859 945
Size: 72 86
Fragments arranged by size:
225 147 141 114 86 72 54 54
36 16
NcoI C'CATG_G
Cuts at: 0 362 945
Size: 362 583
NspI r_CATG'y
Cuts at: 0 792 945
Size: 792 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 270 945
Size: 270 675
PfoI T'CCnGG_A
Cuts at: 0 257 945
Size: 257 688
PshAI GACnn'nnGTC
Cuts at: 0 751 945
Size: 751 194
PstI C_TGCA'G
Cuts at: 0 520 945
Size: 520 425
SexAI A'CCwGG_T
Cuts at: 0 263 945
Size: 263 682
SfcI C'TryA_G
Cuts at: 0 516 879 945
Size: 516 363 66
Fragments arranged by size:
516 363 66
StuI AGG'CCT
Cuts at: 0 721 945
Size: 721 224
StyI C'CwwG_G
Cuts at: 0 362 945
Size: 362 583
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 262 945
Size: 262 683
TatI w'GTAC_w
Cuts at: 0 188 945
Size: 188 757
Tth111I GACn'n_nGTC
Cuts at: 0 265 945
Size: 265 680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 945
Size: 255 690
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApoI BanII
BbsI BglI Bme1580I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI Bsp1286I BspHI BspMI BstYI BtgI EaeI
EarI Eco57MI EcoNI HincII HpaI MfeI MmeI MscI
MslI NcoI NspI PflMI PfoI PshAI PstI SexAI
SfcI StuI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaBI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HindIII KasI KpnI MluI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XhoI XmaI XmnI ZraI