################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BanI BbeI BbrPI BbsI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 698 GACGTC GACGT^C AcyI 698 GACGTC G[GA]^CG[CT]C AseI 900 ATTAAT AT^TAAT AsnI 900 ATTAAT AT^TAAT Asp700I 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 95 TGGCCA TGG^CCA BamHI 446 GGATCC G^GATCC BanII 453 GGGCTC G[GA]GC[CT]^C BbvCI 186 CCTCAGC CCTCAGC(-5/-2) BbvI 263 GCAGC GCAGC(8/12) BciVI 153 GGATAC GTATCC(6/5) BmyI 453 GGGCTC G[AGT]GC[ACT]^C BsaHI 698 GACGTC G[GA]^CG[CT]C BsgI 222 CTGCAC GTGCAG(16/14) Bsp1286I 453 GGGCTC G[AGT]GC[ACT]^C BspHI 404 TCATGA T^CATGA BspMI 760 GCAGGT ACCTGC(4/8) BstXI 272 CCATTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 283 GCTGGC GC[ACGT]^[ACGT]GC EaeI 95 TGGCCA [CT]^GGCC[GA] Fnu4HI 263 GCAGC GC^[ACGT]GC Hin1I 698 GACGTC G[GA]^CG[CT]C HindIII 707 AAGCTT A^AGCTT HphI 729 TCACC GGTGA(8/7) ItaI 263 GCAGC GC^[ACGT]GC MaeII 699 ACGT A^CGT MluNI 95 TGGCCA TGG^CCA MscI 95 TGGCCA TGG^CCA MseI 901 TTAA T^TAA PaeR7I 656 CTCGAG C^TCGAG PshBI 900 ATTAAT AT^TAAT RcaI 404 TCATGA T^CATGA SfaNI 475 GATGC GCATC(5/9) SmlI 656 CTCGAG C^T[CT][GA]AG SspI 579 AATATT AAT^ATT TaqI 657 TCGA T^CGA Tru9I 901 TTAA T^TAA TseI 263 GCAGC G^C[AT]GC TthHB8I 657 TCGA T^CGA XcmI 98 CCATCTTTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 656 CTCGAG C^TCGAG XmnI 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 876 CCGC AcsI 299 AAATTT [GA]^AATT[CT] 416 AAATTT AluI 161 AGCT AG^CT 708 AGCT ApoI 299 AAATTT [GA]^AATT[CT] 416 AAATTT AvaI 512 CTCGGG C^[CT]CG[GA]G 656 CTCGAG AvaII 90 GGTCC G^G[AT]CC 207 GGACC BpmI 689 CTGGAG CTGGAG(16/14) 929 CTGGAG BsaI 408 GAGACC GGTCTC(1/5) 509 GGTCTC BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG 450 CCTGGG BsmAI 408 GAGAC GTCTC(1/5) 510 GTCTC BsmFI 696 GGGAC GGGAC(10/14) 198 GTCCC BsmI 331 GAATGC GAATGC(1/-1) 776 GCATTC BsoBI 512 CTCGGG C^[CT]CG[GA]G 656 CTCGAG BsrDI 317 CATTGC GCAATG(2/0) 566 GCAATG BstYI 446 GGATCC [GA]^GATC[CT] 685 GGATCT Cfr13I 90 GGTCC G^G[ACGT]CC 207 GGACC DpnII 447 GATC ^GATC 686 GATC EarI 79 CTCTTC CTCTTC(1/4) 586 GAAGAG Ksp632I 79 CTCTTC CTCTTC(1/4) 586 GAAGAG MboI 447 GATC ^GATC 686 GATC MflI 446 GGATCC [GA]^GATC[CT] 685 GGATCT MwoI 283 GCTGGCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 326 GCTCCGAATGC NdeII 447 GATC ^GATC 686 GATC NlaIV 325 GGCTCC GG[ACGT]^[ACGT]CC 446 GGATCC Sau3AI 447 GATC ^GATC 686 GATC Sau96I 90 GGTCC G^G[ACGT]CC 207 GGACC SfcI 458 CTACAG C^T[GA][CT]AG 479 CTGTAG Tsp509I 300 AATT ^AATT 417 AATT XhoII 446 GGATCC [GA]^GATC[CT] 685 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AfaI AlwI BseRI BsiYI BslI BssKI BstNI DdeI EcoRII HaeIII HinfI MaeIII MboII MnlI MslI MvaI NlaIII RsaI ScrFI TfiI Tsp45I TspRI