################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI AlwI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI BspEI BsrBI BssHII Bst1107I BstAPI BstEII BstPI BstXI BstZ17I CelII CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MluI MroI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI SspI SwaI Tsp45I Tsp509I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccII 274 CGCG CG^CG Alw44I 503 GTGCAC G^TGCAC AocI 417 CCTGAGG CC^T[ACGT]AGG ApaLI 503 GTGCAC G^TGCAC AspHI 503 GTGCAC G[AT]GC[AT]^C AspI 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 36 CCTAGG C^CTAGG BalI 89 TGGCCA TGG^CCA BanII 59 GAGCCC G[GA]GC[CT]^C BbsI 574 GTCTTC GAAGAC(2/6) BfaI 37 CTAG C^TAG BglII 293 AGATCT A^GATCT BlnI 36 CCTAGG C^CTAGG BpuAI 574 GTCTTC GAAGAC(2/6) BsaWI 253 ACCGGA [AT]^CCGG[AT] BsiHKAI 503 GTGCAC G[AT]GC[AT]^C Bsp1407I 590 TGTACA T^GTACA BspDI 514 ATCGAT AT^CGAT BspLU11I 723 ACATGT A^CATGT BspMI 712 ACCTGC ACCTGC(4/8) BsrDI 116 GCAATG GCAATG(2/0) BsrFI 152 ACCGGC [GA]^CCGG[CT] BsrGI 590 TGTACA T^GTACA BssSI 508 CACGAG CACGAG(-5/-1) BstBI 577 TTCGAA TT^CGAA BstUI 274 CGCG CG^CG BstYI 293 AGATCT [GA]^GATC[CT] Bsu36I 417 CCTGAGG CC^T[ACGT]AGG Cfr10I 152 ACCGGC [GA]^CCGG[CT] ClaI 514 ATCGAT AT^CGAT DraII 533 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 311 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 344 CCATGG C^C[GA][CT]GG EaeI 89 TGGCCA [CT]^GGCC[GA] Eco81I 417 CCTGAGG CC^T[ACGT]AGG EcoNI 462 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 533 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRV 386 GATATC GAT^ATC HphI 836 TCACC GGTGA(8/7) MaeI 37 CTAG C^TAG MflI 293 AGATCT [GA]^GATC[CT] MluNI 89 TGGCCA TGG^CCA MscI 89 TGGCCA TGG^CCA MvnI 274 CGCG CG^CG NcoI 344 CCATGG C^CATGG NspI 723 ACATGT [GA]CATG^[CT] NspV 577 TTCGAA TT^CGAA PflFI 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 466 CTGCAG CTGCA^G SapI 716 GCTCTTC GCTCTTC(1/4) SbfI 465 CCTGCAGG CCTGCA^GG SfuI 577 TTCGAA TT^CGAA Sse8387I 465 CCTGCAGG CCTGCA^GG SspBI 590 TGTACA T^GTACA StuI 701 AGGCCT AGG^CCT Tth111I 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 293 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AfaI 171 GTAC GT^AC 591 GTAC AflIII 723 ACATGT A^C[GA][CT]GT 913 ACGTGT BsaI 774 GAGACC GGTCTC(1/5) 649 GGTCTC BsgI 289 GTGCAG GTGCAG(16/14) 449 GTGCAG BsmAI 774 GAGAC GTCTC(1/5) 650 GTCTC BsrI 143 ACTGG ACTGG(1/-1) 312 ACTGG CfoI 275 GCGC GCG^C 678 GCGC DrdI 426 GACCATGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 565 GACACTTCAGTC EarI 73 CTCTTC CTCTTC(1/4) 717 CTCTTC EcoT14I 36 CCTAGG C^C[AT][AT]GG 344 CCATGG FauI 272 CCCGC CCCGC(4/6) 692 CCCGC HapII 153 CCGG C^CGG 254 CCGG HhaI 275 GCGC GCG^C 678 GCGC HinP1I 275 GCGC G^CGC 678 GCGC HpaII 153 CCGG C^CGG 254 CCGG Ksp632I 73 CTCTTC CTCTTC(1/4) 717 CTCTTC MaeII 203 ACGT A^CGT 913 ACGT MseI 47 TTAA T^TAA 607 TTAA MspA1I 409 CAGCTG C[AC]G^C[GT]G 452 CAGCTG MspI 153 CCGG C^CGG 254 CCGG MwoI 156 GCTCCACACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 340 GCAGCCATGGC PleI 41 GACTC GAGTC(4/5) 740 GACTC PvuII 409 CAGCTG CAG^CTG 452 CAGCTG RsaI 171 GTAC GT^AC 591 GTAC SfaNI 81 GATGC GCATC(5/9) 207 GATGC StyI 36 CCTAGG C^C[AT][AT]GG 344 CCATGG TaqI 515 TCGA T^CGA 578 TCGA TfiI 129 GATTC G^A[AT]TC 138 GATTC Tru9I 47 TTAA T^TAA 607 TTAA TthHB8I 515 TCGA T^CGA 578 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AvaII BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BspHI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MboI MboII MnlI MslI MvaI NdeII NlaIII NlaIV RcaI Sau3AI Sau96I ScrFI SfcI TseI TspRI