################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BanI BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tru9I Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AflIII 71 ACACGT A^C[GA][CT]GT Asp700I 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 446 GGATCC G^GATCC BanII 453 GGGCTC G[GA]GC[CT]^C BbvCI 186 CCTCAGC CCTCAGC(-5/-2) BclI 792 TGATCA T^GATCA BglII 908 AGATCT A^GATCT BmyI 453 GGGCTC G[AGT]GC[ACT]^C BpmI 689 CTGGAG CTGGAG(16/14) BsaI 509 GGTCTC GGTCTC(1/5) BsgI 222 CTGCAC GTGCAG(16/14) BsmAI 510 GTCTC GTCTC(1/5) BsmFI 198 GTCCC GGGAC(10/14) BsmI 898 GCATTC GAATGC(1/-1) Bsp1286I 453 GGGCTC G[AGT]GC[ACT]^C BstXI 272 CCATTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoRI 538 GAATTC G^AATTC EcoT14I 42 CCTTGG C^C[AT][AT]GG FbaI 792 TGATCA T^GATCA HindIII 707 AAGCTT A^AGCTT MaeII 73 ACGT A^CGT NlaIV 446 GGATCC GG[ACGT]^[ACGT]CC PaeR7I 656 CTCGAG C^TCGAG SmlI 656 CTCGAG C^T[CT][GA]AG SspI 579 AATATT AAT^ATT StyI 42 CCTTGG C^C[AT][AT]GG TaqI 657 TCGA T^CGA TspRI 317 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 657 TCGA T^CGA XhoI 656 CTCGAG C^TCGAG XmnI 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 876 CCGC AvaI 512 CTCGGG C^[CT]CG[GA]G 656 CTCGAG AvaII 90 GGTCC G^G[AT]CC 207 GGACC BbvI 263 GCAGC GCAGC(8/12) 760 GCAGC BfaI 893 CTAG C^TAG 1018 CTAG BseRI 139 CTCCTC GAGGAG(10/8) 184 CTCCTC BsoBI 512 CTCGGG C^[CT]CG[GA]G 656 CTCGAG Bsp1407I 773 TGTACA T^GTACA 1031 TGTACA BspHI 404 TCATGA T^CATGA 902 TCATGA BsrGI 773 TGTACA T^GTACA 1031 TGTACA BstF5I 36 CATCC GGATG(2/0) 837 CATCC EarI 79 CTCTTC CTCTTC(1/4) 586 GAAGAG Fnu4HI 263 GCAGC GC^[ACGT]GC 760 GCAGC FokI 36 CATCC GGATG(9/13) 837 CATCC HphI 888 GGTGA GGTGA(8/7) 729 TCACC ItaI 263 GCAGC GC^[ACGT]GC 760 GCAGC Ksp632I 79 CTCTTC CTCTTC(1/4) 586 GAAGAG MaeI 893 CTAG C^TAG 1018 CTAG MwoI 346 GCTGTTATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 646 GCTTCCTATGC RcaI 404 TCATGA T^CATGA 902 TCATGA SfaNI 475 GATGC GCATC(5/9) 98 GCATC SspBI 773 TGTACA T^GTACA 1031 TGTACA TseI 263 GCAGC G^C[AT]GC 760 GCAGC Tsp45I 292 GTCAC ^GT[GC]AC 889 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BsaJI BsiYI BslI BsrDI BssKI BstNI BstYI Cfr13I DdeI DpnII EcoRII HaeIII HinfI MaeIII MboI MboII MflI MnlI MslI MvaI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfcI TfiI Tsp509I XhoII