(Circular) (Six-base) MAPSORT of: pFN21AE5493.seq Check: 6921 from: 1 to: 5746 pFN21AE5493 5746 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3089 1937 Size: 1152 4594 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3809 5193 5461 1632 Size: 2177 1384 268 1917 Fragments arranged by size: 2177 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 2076 4645 5622 1203 Size: 873 2569 977 1327 Fragments arranged by size: 2569 1327 977 873 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AlwNI CAG_nnn'CTG Cuts at: 3489 5061 3489 Size: 1572 4174 ApaLI G'TGCA_C Cuts at: 3803 4959 3803 Size: 1156 4590 ApoI r'AATT_y Cuts at: 1493 2304 2421 2543 2978 3055 3281 3433 5542 Size: 811 117 122 435 77 226 152 2109 Cuts at: 5542 1493 Size: 1697 Fragments arranged by size: 2109 1697 811 435 226 152 122 117 77 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2517 2661 3071 1870 Size: 73 574 144 410 4545 Fragments arranged by size: 4545 574 410 144 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2220 2253 2220 Size: 33 5713 BamHI G'GATC_C Cuts at: 2451 3076 3443 2451 Size: 625 367 4754 Fragments arranged by size: 4754 625 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3067 4522 618 Size: 325 217 627 1280 1455 1842 Fragments arranged by size: 1842 1455 1280 627 325 217 BanII G_rGCy'C Cuts at: 1391 2462 3065 1391 Size: 1071 603 4072 Fragments arranged by size: 4072 1071 603 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4775 BbvCI CC'TCA_GC Cuts at: 2192 2192 Size: 5746 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 1903 Size: 34 2000 34 3678 Fragments arranged by size: 3678 2000 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 2797 1548 Size: 1249 4497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4357 136 Size: 107 122 71 631 605 2685 1525 Fragments arranged by size: 2685 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2913 5741 2913 Size: 2828 2918 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 3807 4963 1790 Size: 2017 1156 2573 Fragments arranged by size: 2573 2017 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2715 4406 1239 Size: 1476 1691 2579 Fragments arranged by size: 2579 1691 1476 Bpu10I CC'TnA_GC Cuts at: 2192 2192 Size: 5746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 4736 5034 3871 Size: 865 298 4583 Fragments arranged by size: 4583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2520 4409 882 Size: 617 1021 1889 2219 Fragments arranged by size: 2219 1889 1021 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3553 3935 4570 275 Size: 53 83 186 936 2020 382 635 1451 Fragments arranged by size: 2020 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5521 Fragments arranged by size: 5521 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2180 1186 Size: 949 45 4752 Fragments arranged by size: 4752 949 45 BseYI C'CCAG_C Cuts at: 2163 4949 5445 2163 Size: 2786 496 2464 Fragments arranged by size: 2786 2464 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2212 1163 Size: 573 476 4697 Fragments arranged by size: 4697 573 476 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3807 3892 4963 3807 Size: 85 1071 4590 Fragments arranged by size: 4590 1071 85 BsmI GAATG_Cn' Cuts at: 2903 3257 3350 2903 Size: 354 93 5299 Fragments arranged by size: 5299 354 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2462 3065 3807 3892 4963 1391 Size: 399 672 603 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 672 603 399 85 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 2409 2907 3635 5365 2409 Size: 498 728 1730 2790 Fragments arranged by size: 2790 1730 728 498 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3101 844 Size: 2257 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2578 2646 3039 4241 4415 65 Size: 1183 1330 68 393 1202 174 1396 Fragments arranged by size: 1396 1330 1202 1183 393 174 68 BsrFI r'CCGG_y Cuts at: 1700 1810 4390 4577 1700 Size: 110 2580 187 2869 Fragments arranged by size: 2869 2580 187 110 BsrGI T'GTAC_A Cuts at: 96 2778 3036 96 Size: 2682 258 2806 Fragments arranged by size: 2806 2682 258 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5746 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5746 BstXI CCAn_nnnn'nTGG Cuts at: 2284 2284 Size: 5746 BstYI r'GATC_y Cuts at: 1972 2451 2690 2913 3076 3443 3828 3845 5297 Size: 479 239 223 163 367 385 17 1452 Cuts at: 5297 5383 5395 5741 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 479 385 367 346 239 223 163 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4255 Fragments arranged by size: 4255 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 3262 4056 4076 3262 Size: 794 20 4932 Fragments arranged by size: 4932 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3193 4082 5711 Size: 54 1425 50 275 18 1363 889 1629 Cuts at: 5711 8 Size: 43 Fragments arranged by size: 1629 1425 1363 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2586 3676 1353 Size: 737 496 1090 3423 Fragments arranged by size: 3423 1090 737 496 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2715 3809 4406 5193 5461 1239 Size: 393 1083 1094 597 787 268 1524 Fragments arranged by size: 1524 1094 1083 787 597 393 268 EcoRI G'AATT_C Cuts at: 2543 3055 3433 2543 Size: 512 378 4856 Fragments arranged by size: 4856 512 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2266 2266 Size: 5746 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 4893 1678 Size: 3215 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4387 4419 4461 4493 1373 Size: 32 135 32 2815 32 42 32 2626 Fragments arranged by size: 2815 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3336 669 Size: 988 281 1152 246 3079 Fragments arranged by size: 3079 1152 988 281 246 HindIII A'AGCT_T Cuts at: 748 2712 748 Size: 1964 3782 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 4860 5044 1305 Size: 753 2802 184 2007 Fragments arranged by size: 2802 2007 753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3924 Fragments arranged by size: 3924 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2282 2684 2744 2852 3704 4063 Size: 648 1011 105 402 60 108 852 359 Cuts at: 4063 4222 518 Size: 159 2042 Fragments arranged by size: 2042 1011 852 648 402 359 159 108 105 60 MspA1I CmG'CkG Cuts at: 1606 3162 3839 4987 5232 1606 Size: 1556 677 1148 245 2120 Fragments arranged by size: 2120 1556 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4255 Fragments arranged by size: 4255 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 3104 4649 1132 Size: 97 1875 1545 2229 Fragments arranged by size: 2229 1875 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5746 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4844 PfoI T'CCnGG_A Cuts at: 2447 2447 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PspXI vC'TCGA_Gb Cuts at: 2661 2661 Size: 5746 PstI C_TGCA'G Cuts at: 830 3098 830 Size: 2268 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 2463 2484 2872 3094 4229 4910 5101 Size: 219 1418 21 388 222 1135 681 191 Cuts at: 5101 826 Size: 1471 Fragments arranged by size: 1471 1418 1135 681 388 222 219 191 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 3073 1872 Size: 1201 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2661 3850 4751 5013 820 Size: 197 926 718 1189 901 262 1553 Fragments arranged by size: 1553 1189 926 901 718 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 2586 3670 5 Size: 47 2534 1084 2081 Fragments arranged by size: 2534 2081 1084 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 2004 2047 3185 513 Size: 552 939 43 1138 3074 Fragments arranged by size: 3074 1138 939 552 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2177 3773 3790 3943 4128 4573 325 Size: 1255 597 1596 17 153 185 445 1498 Fragments arranged by size: 1596 1498 1255 597 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2754 2778 Size: 274 80 33 51 1443 204 573 24 Cuts at: 2778 3036 3992 5704 96 Size: 258 956 1712 138 Fragments arranged by size: 1712 1443 956 573 274 258 204 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2064 2096 5706 5738 2064 Size: 32 3610 32 2072 Fragments arranged by size: 3610 2072 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 2661 1943 Size: 718 5028 XmaI C'CCGG_G Cuts at: 1870 3071 1870 Size: 1201 4545 XmnI GAAnn'nnTTC Cuts at: 2340 3875 2340 Size: 1535 4211 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI