################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BspDI BspEI BspLU11I BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tru9I Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AspHI 896 GAGCAC G[AT]GC[AT]^C BalI 95 TGGCCA TGG^CCA BamHI 446 GGATCC G^GATCC BanII 453 GGGCTC G[GA]GC[CT]^C BbvI 263 GCAGC GCAGC(8/12) BpmI 689 CTGGAG CTGGAG(16/14) BsiHKAI 896 GAGCAC G[AT]GC[AT]^C BsmI 331 GAATGC GAATGC(1/-1) Bsp1407I 773 TGTACA T^GTACA BspHI 404 TCATGA T^CATGA BspMI 760 GCAGGT ACCTGC(4/8) BsrDI 566 GCAATG GCAATG(2/0) BsrGI 773 TGTACA T^GTACA BstEII 888 GGTGACC G^GT[ACGT]ACC BstPI 888 GGTGACC G^GT[ACGT]ACC BstXI 272 CCATTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 95 TGGCCA [CT]^GGCC[GA] EarI 79 CTCTTC CTCTTC(1/4) EcoO65I 888 GGTGACC G^GT[ACGT]ACC Fnu4HI 263 GCAGC GC^[ACGT]GC HincII 191 GTCAAC GT[CT]^[GA]AC HindII 191 GTCAAC GT[CT]^[GA]AC HindIII 707 AAGCTT A^AGCTT ItaI 263 GCAGC GC^[ACGT]GC Ksp632I 79 CTCTTC CTCTTC(1/4) MluNI 95 TGGCCA TGG^CCA MscI 95 TGGCCA TGG^CCA PaeR7I 656 CTCGAG C^TCGAG RcaI 404 TCATGA T^CATGA SmlI 656 CTCGAG C^T[CT][GA]AG SspBI 773 TGTACA T^GTACA SspI 579 AATATT AAT^ATT TaqI 657 TCGA T^CGA TseI 263 GCAGC G^C[AT]GC TthHB8I 657 TCGA T^CGA XcmI 98 CCATCTTTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 656 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 876 CCGC AcsI 299 AAATTT [GA]^AATT[CT] 416 AAATTT ApoI 299 AAATTT [GA]^AATT[CT] 416 AAATTT Asp700I 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 586 GAAAAGGTTC AvaI 512 CTCGGG C^[CT]CG[GA]G 656 CTCGAG AvaII 90 GGTCC G^G[AT]CC 207 GGACC BmyI 453 GGGCTC G[AGT]GC[ACT]^C 896 GAGCAC BsaI 408 GAGACC GGTCTC(1/5) 509 GGTCTC BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG 450 CCTGGG BseRI 139 CTCCTC GAGGAG(10/8) 184 CTCCTC BsmAI 408 GAGAC GTCTC(1/5) 510 GTCTC BsoBI 512 CTCGGG C^[CT]CG[GA]G 656 CTCGAG Bsp1286I 453 GGGCTC G[AGT]GC[ACT]^C 896 GAGCAC BstYI 446 GGATCC [GA]^GATC[CT] 685 GGATCT HphI 888 GGTGA GGTGA(8/7) 729 TCACC MaeIII 536 GTGAC ^GT[ACGT]AC 889 GTGAC MflI 446 GGATCC [GA]^GATC[CT] 685 GGATCT NlaIV 325 GGCTCC GG[ACGT]^[ACGT]CC 446 GGATCC SfaNI 463 GATGC GCATC(5/9) 475 GATGC SfcI 479 CTGTAG C^T[GA][CT]AG 867 CTACAG Tsp45I 536 GTGAC ^GT[GC]AC 889 GTGAC Tsp509I 300 AATT ^AATT 417 AATT TspRI 107 CAGTG CA[GC]TG[ACGT][ACGT]^ 317 CACTG XhoII 446 GGATCC [GA]^GATC[CT] 685 GGATCT XmnI 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 586 GAAAAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AfaI AluI AlwI BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII FokI HaeIII HinfI MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI TfiI