################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssKI BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 633 GTATAC GT^[AC][GT]AC AciI 862 GCGG CCGC(-3/-1) AlwI 180 GATCC GGATC(4/5) Asp700I 376 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 729 GAGCTC G[AT]GC[AT]^C BanII 729 GAGCTC G[GA]GC[CT]^C BbvI 467 GCTGC GCAGC(8/12) BfaI 679 CTAG C^TAG BglI 682 GCCATTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 554 GGTCTC GGTCTC(1/5) BseRI 948 GAGGAG GAGGAG(10/8) BsiHKAI 729 GAGCTC G[AT]GC[AT]^C BsmFI 70 GGGAC GGGAC(10/14) Bsp1407I 260 TGTACA T^GTACA BsrDI 135 CATTGC GCAATG(2/0) BsrGI 260 TGTACA T^GTACA BssSI 701 CTCGTG CACGAG(-5/-1) Bst1107I 633 GTATAC GTA^TAC BstZ17I 633 GTATAC GTA^TAC Cac8I 787 GCAAGC GC[ACGT]^[ACGT]GC EarI 119 CTCTTC CTCTTC(1/4) EcoNI 818 CCTACACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Fnu4HI 467 GCTGC GC^[ACGT]GC HincII 250 GTTGAC GT[CT]^[GA]AC HindII 250 GTTGAC GT[CT]^[GA]AC HphI 426 TCACC GGTGA(8/7) ItaI 467 GCTGC GC^[ACGT]GC Ksp632I 119 CTCTTC CTCTTC(1/4) MaeI 679 CTAG C^TAG MaeII 346 ACGT A^CGT MseI 566 TTAA T^TAA NspI 481 GCATGT [GA]CATG^[CT] PleI 206 GACTC GAGTC(4/5) SacI 729 GAGCTC GAGCT^C SspBI 260 TGTACA T^GTACA TaqI 192 TCGA T^CGA TfiI 339 GATTC G^A[AT]TC Tru9I 566 TTAA T^TAA TseI 467 GCTGC G^C[AT]GC TthHB8I 192 TCGA T^CGA XcmI 317 CCATTAGATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 376 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 222 GTAC GT^AC 261 GTAC BglII 461 AGATCT A^GATCT 994 AGATCT BmyI 277 GTGCCC G[AGT]GC[ACT]^C 729 GAGCTC BsaJI 363 CCTTGG C^C[ACGT][ACGT]GG 387 CCTTGG BsmAI 951 GAGAC GTCTC(1/5) 555 GTCTC Bsp1286I 277 GTGCCC G[AGT]GC[ACT]^C 729 GAGCTC BsrI 494 CCAGT ACTGG(1/-1) 624 CCAGT BstYI 461 AGATCT [GA]^GATC[CT] 994 AGATCT Cfr13I 360 GGCCC G^G[ACGT]CC 491 GGGCC EcoT14I 363 CCTTGG C^C[AT][AT]GG 387 CCTTGG HinfI 206 GACTC G^A[ACGT]TC 339 GATTC MboII 459 GAAGA GAAGA(8/7) 120 TCTTC MflI 461 AGATCT [GA]^GATC[CT] 994 AGATCT RsaI 222 GTAC GT^AC 261 GTAC Sau96I 360 GGCCC G^G[ACGT]CC 491 GGGCC StyI 363 CCTTGG C^C[AT][AT]GG 387 CCTTGG XhoII 461 AGATCT [GA]^GATC[CT] 994 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI BsiYI BslI DdeI DpnII HaeIII MaeIII MboI MnlI MslI MwoI NdeII NlaIII Sau3AI SfcI Tsp45I TspRI