################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 99 CGCG CG^CG AccIII 554 TCCGGA T^CCGGA AcsI 680 AAATTC [GA]^AATT[CT] AflIII 770 ACATGT A^C[GA][CT]GT AgeI 362 ACCGGT A^CCGGT AlwNI 414 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 680 AAATTC [GA]^AATT[CT] Asp700I 705 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 74 GGATCC G^GATCC BclI 401 TGATCA T^GATCA BfaI 931 CTAG C^TAG BpmI 67 CTGGAG CTGGAG(16/14) BsaI 741 GGTCTC GGTCTC(1/5) BseAI 554 TCCGGA T^CCGGA BseRI 850 CTCCTC GAGGAG(10/8) BsgI 388 CTGCAC GTGCAG(16/14) Bsp1407I 773 TGTACA T^GTACA BspEI 554 TCCGGA T^CCGGA BspHI 404 TCATGA T^CATGA BspLU11I 770 ACATGT A^CATGT BspMI 718 ACCTGC ACCTGC(4/8) BsrFI 362 ACCGGT [GA]^CCGG[CT] BsrGI 773 TGTACA T^GTACA BssSI 514 CACGAG CACGAG(-5/-1) BstUI 99 CGCG CG^CG BstYI 74 GGATCC [GA]^GATC[CT] CfoI 492 GCGC GCG^C Cfr10I 362 ACCGGT [GA]^CCGG[CT] DraIII 514 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 586 GACGGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 401 TGATCA T^GATCA HhaI 492 GCGC GCG^C HinP1I 492 GCGC G^CGC HinfI 47 GATTC G^A[ACGT]TC MaeI 931 CTAG C^TAG MflI 74 GGATCC [GA]^GATC[CT] MroI 554 TCCGGA T^CCGGA MseI 254 TTAA T^TAA MvnI 99 CGCG CG^CG PflMI 513 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 362 ACCGGT A^CCGGT PvuII 238 CAGCTG CAG^CTG RcaI 404 TCATGA T^CATGA SspBI 773 TGTACA T^GTACA StuI 707 AGGCCT AGG^CCT TaqI 584 TCGA T^CGA TfiI 47 GATTC G^A[AT]TC Tru9I 254 TTAA T^TAA Tsp509I 681 AATT ^AATT TthHB8I 584 TCGA T^CGA Van91I 513 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 74 GGATCC [GA]^GATC[CT] XmnI 705 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BcnI 146 CCCGG CC^[GC]GG 423 CCGGG BmyI 508 GGGCAC G[AGT]GC[ACT]^C 896 GGGCAC BsaWI 362 ACCGGT [AT]^CCGG[AT] 554 TCCGGA BsmAI 477 GTCTC GTCTC(1/5) 742 GTCTC BsmFI 144 GTCCC GGGAC(10/14) 624 GTCCC Bsp1286I 508 GGGCAC G[AGT]GC[ACT]^C 896 GGGCAC BstXI 131 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 461 CCAACTCCTTGG DdeI 211 CTCAG C^T[ACGT]AG 874 CTCAG EcoT14I 467 CCTTGG C^C[AT][AT]GG 629 CCTTGG FauI 500 CCCGC CCCGC(4/6) 544 CCCGC MspA1I 238 CAGCTG C[AC]G^C[GT]G 501 CCGCTG NciI 146 CCCGG CC^[GC]GG 423 CCGGG NspI 770 ACATGT [GA]CATG^[CT] 776 ACATGC SapI 300 GCTCTTC GCTCTTC(1/4) 825 GCTCTTC StyI 467 CCTTGG C^C[AT][AT]GG 629 CCTTGG XcmI 128 CCACCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 272 CCATCAGCTACATGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BalI BanI BbvI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EaeI EarI EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MslI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI