################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1286I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeIII Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboII MfeI MluI MluNI MscI MseI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 218 TCCGGA T^CCGGA AfaI 345 GTAC GT^AC Aor51HI 125 AGCGCT AGC^GCT BbvCI 207 CCTCAGC CCTCAGC(-5/-2) BglII 308 AGATCT A^GATCT BpmI 382 CTGGAG CTGGAG(16/14) BsaWI 218 TCCGGA [AT]^CCGG[AT] BseAI 218 TCCGGA T^CCGGA BsmBI 139 GAGACG CGTCTC(1/5) BsmFI 178 GTCCC GGGAC(10/14) Bsp1407I 344 TGTACA T^GTACA BspEI 218 TCCGGA T^CCGGA BsrGI 344 TGTACA T^GTACA BsrI 201 CCAGT ACTGG(1/-1) BstXI 167 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 308 AGATCT [GA]^GATC[CT] DpnII 309 GATC ^GATC Eco47III 125 AGCGCT AGC^GCT EcoT14I 299 CCATGG C^C[AT][AT]GG HaeII 125 AGCGCT [GA]GCGC^[CT] HgaI 26 GCGTC GACGC(5/10) HindIII 51 AAGCTT A^AGCTT HphI 264 GGTGA GGTGA(8/7) MaeII 142 ACGT A^CGT MboI 309 GATC ^GATC MflI 308 AGATCT [GA]^GATC[CT] MroI 218 TCCGGA T^CCGGA MspA1I 154 CCGCTG C[AC]G^C[GT]G NcoI 299 CCATGG C^CATGG NdeII 309 GATC ^GATC PleI 385 GAGTC GAGTC(4/5) RsaI 345 GTAC GT^AC Sau3AI 309 GATC ^GATC SfaNI 212 GCATC GCATC(5/9) SfcI 312 CTACAG C^T[GA][CT]AG SmlI 415 CTTGAG C^T[CT][GA]AG SspBI 344 TGTACA T^GTACA StyI 299 CCATGG C^C[AT][AT]GG TfiI 83 GAATC G^A[AT]TC XcmI 363 CCAGGAGACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 308 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AluI 52 AGCT AG^CT 188 AGCT AlwNI 184 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 423 CAGACCCTG AvaII 177 GGTCC G^G[AT]CC 231 GGTCC BcnI 87 CCGGG CC^[GC]GG 111 CCCGG BsiYI 397 CCCAGGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 398 CCAGGGATGGG BslI 397 CCCAGGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 398 CCAGGGATGGG CfoI 126 GCGC GCG^C 293 GCGC Cfr13I 177 GGTCC G^G[ACGT]CC 231 GGTCC DdeI 208 CTCAG C^T[ACGT]AG 235 CTGAG DsaI 242 CCACGG C^C[GA][CT]GG 299 CCATGG FauI 153 CCCGC CCCGC(4/6) 180 CCCGC HhaI 126 GCGC GCG^C 293 GCGC HinP1I 126 GCGC G^CGC 293 GCGC HinfI 83 GAATC G^A[ACGT]TC 385 GAGTC NciI 87 CCGGG CC^[GC]GG 111 CCCGG NlaIV 177 GGTCCC GG[ACGT]^[ACGT]CC 221 GGAGCC Sau96I 177 GGTCC G^G[ACGT]CC 231 GGTCC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI BbvI BsaJI BsmAI BssKI BstF5I BstNI EcoRII Fnu4HI FokI HapII HpaII ItaI MnlI MspI MvaI MwoI NlaIII ScrFI TseI