Restriction map for
pFN21AE2559
(Circular) (Six-base) MAPSORT of: pFN21AE2559.seq Check: 630 from: 1 to: 5146
pFN21AE2559 5146 bp
With 182 enzymes: SgfI *
January 9, 2015 16:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2956 278
Size: 53 83 186 936 1420 2468
Fragments arranged by size:
2468 1420 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2489 1937
Size: 552 4594
Acc65I G'GTAC_C
Cuts at: 2467 2467
Size: 5146
AclI AA'CG_TT
Cuts at: 1563 3273 3646 1563
Size: 1710 373 3063
Fragments arranged by size:
3063 1710 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3209 4593 4861 1632
Size: 1577 1384 268 1917
Fragments arranged by size:
1917 1577 1384 268
AfeI AGC'GCT
Cuts at: 2132 2132
Size: 5146
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4949
AflIII A'CryG_T
Cuts at: 1203 4045 5022 1203
Size: 2842 977 1327
Fragments arranged by size:
2842 1327 977
AgeI A'CCGG_T
Cuts at: 3977 3977
Size: 5146
AhdI GACnn_n'nnGTC
Cuts at: 3875 3875
Size: 5146
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5146
AlwNI CAG_nnn'CTG
Cuts at: 2194 2433 2889 4461 2194
Size: 239 456 1572 2879
Fragments arranged by size:
2879 1572 456 239
ApaLI G'TGCA_C
Cuts at: 3203 4359 3203
Size: 1156 3990
ApoI r'AATT_y
Cuts at: 1493 2455 2681 2833 4942 1493
Size: 962 226 152 2109 1697
Fragments arranged by size:
2109 1697 962 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3700 160
Size: 872 2668 1606
Fragments arranged by size:
2668 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5146
AvaI C'yCGr_G
Cuts at: 1870 1943 2471 1870
Size: 73 528 4545
Fragments arranged by size:
4545 528 73
BamHI G'GATC_C
Cuts at: 2476 2843 2476
Size: 367 4779
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2467 3922 618
Size: 325 217 627 680 1455 1842
Fragments arranged by size:
1842 1455 680 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2465 1391
Size: 1074 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4175
BbvCI CC'TCA_GC
Cuts at: 2213 2213
Size: 5146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2425 2459 3337 3371 1903
Size: 34 50 34 404 34 878 34 3678
Fragments arranged by size:
3678 878 404 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3038 4248 3038
Size: 1210 3936
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5146
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3757 136
Size: 107 122 71 631 605 2085 1525
Fragments arranged by size:
2085 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2313 5141 2313
Size: 2828 2318
BlpI GC'TnA_GC
Cuts at: 2563 2563
Size: 5146
Bme1580I G_kGCm'C
Cuts at: 1790 3207 4363 1790
Size: 1417 1156 2573
Fragments arranged by size:
2573 1417 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5146
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2200 2877 3835
Size: 416 53 83 399 24 783 677 958
Cuts at: 3835 442
Size: 1753
Fragments arranged by size:
1753 958 783 677 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 3806 1239
Size: 1169 1398 2579
Fragments arranged by size:
2579 1398 1169
Bpu10I CC'TnA_GC
Cuts at: 2213 2213
Size: 5146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2441 3271 4136 4434 2441
Size: 830 865 298 3153
Fragments arranged by size:
3153 865 830 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3809 882
Size: 617 2310 2219
Fragments arranged by size:
2310 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2937 493
Size: 711 1733 2702
Fragments arranged by size:
2702 1733 711
BsaBI GATnn'nnATC
Cuts at: 5140 5140
Size: 5146
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2953 3335 3970 275
Size: 53 83 186 936 1420 382 635 1451
Fragments arranged by size:
1451 1420 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2223 3579 3977 4251 4398 4809 2223
Size: 1356 398 274 147 411 2560
Fragments arranged by size:
2560 1356 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4921
Fragments arranged by size:
4921 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5146
BseYI C'CCAG_C
Cuts at: 4349 4845 4349
Size: 496 4650
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2596 3357 4385 1540
Size: 275 186 595 761 1028 2301
Fragments arranged by size:
2301 1028 761 595 275 186
BsiHKAI G_wGCw'C
Cuts at: 3207 3292 4363 3207
Size: 85 1071 3990
Fragments arranged by size:
3990 1071 85
BsmI GAATG_Cn'
Cuts at: 2657 2750 2657
Size: 93 5053
BsmBI CGTCTCn'nnnn_
Cuts at: 2138 2138
Size: 5146
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2465 3207 3292 4363 1391
Size: 399 675 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 675 399 85
BspEI T'CCGG_A
Cuts at: 2223 4809 2223
Size: 2586 2560
BspHI T'CATG_A
Cuts at: 3035 4765 3035
Size: 1730 3416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2501 844
Size: 1657 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3033 1394
Size: 429 1210 3507
Fragments arranged by size:
3507 1210 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3641 3815 65
Size: 1183 2393 174 1396
Fragments arranged by size:
2393 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3790 3977 1700
Size: 110 1980 187 2869
Fragments arranged by size:
2869 1980 187 110
BsrGI T'GTAC_A
Cuts at: 96 2349 96
Size: 2253 2893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5146
BssSI C'ACGA_G
Cuts at: 3206 4218 3206
Size: 1012 4134
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5146
BstBI TT'CG_AA
Cuts at: 3950 3950
Size: 5146
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5146
BstXI CCAn_nnnn'nTGG
Cuts at: 2179 2179
Size: 5146
BstYI r'GATC_y
Cuts at: 1972 2313 2476 2843 3228 3245 4697 4783 4795
Size: 341 163 367 385 17 1452 86 12
Cuts at: 4795 5141 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 341 163 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2247 2304 513
Size: 552 1182 57 3355
Fragments arranged by size:
3355 1182 552 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4407
Fragments arranged by size:
4407 683 56
BtsI GCAGTG_nn'
Cuts at: 2662 3456 3476 2662
Size: 794 20 4332
Fragments arranged by size:
4332 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5146
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5111
DraI TTT'AAA
Cuts at: 2450 2450
Size: 5146
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4153 809
Size: 507 2837 1802
Fragments arranged by size:
2837 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2593 3482 5111
Size: 54 1425 50 275 18 763 889 1629
Cuts at: 5111 8
Size: 43
Fragments arranged by size:
1629 1425 889 763 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2593 1537
Size: 275 781 4090
Fragments arranged by size:
4090 781 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3076 1353
Size: 1723 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3733 4107 4253 387
Size: 1064 138 2144 374 146 1280
Fragments arranged by size:
2144 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 3209 3806 4593 4861 1239
Size: 393 776 801 597 787 268 1524
Fragments arranged by size:
1524 801 787 776 597 393 268
EcoRI G'AATT_C
Cuts at: 2455 2833 2455
Size: 378 4768
FspI TGC'GCA
Cuts at: 3652 3652
Size: 5146
HaeII r_GCGC'y
Cuts at: 1678 2134 4293 1678
Size: 456 2159 2531
Fragments arranged by size:
2531 2159 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3787 3819 3861 3893 1373
Size: 32 135 32 2215 32 42 32 2626
Fragments arranged by size:
2626 2215 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2490 2736 669
Size: 988 281 552 246 3079
Fragments arranged by size:
3079 988 552 281 246
HindIII A'AGCT_T
Cuts at: 748 2056 748
Size: 1308 3838
HpaI GTT'AAC
Cuts at: 2736 2736
Size: 5146
KpnI G_GTAC'C
Cuts at: 2471 2471
Size: 5146
MfeI C'AATT_G
Cuts at: 2745 2745
Size: 5146
MluI A'CGCG_T
Cuts at: 5022 5022
Size: 5146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4260 4444 1305
Size: 2955 184 2007
Fragments arranged by size:
2955 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3324
Fragments arranged by size:
3324 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3104 3463 3622 518
Size: 648 1938 359 159 2042
Fragments arranged by size:
2042 1938 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 2562 3239 4387 4632 1606
Size: 555 401 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 555 401 245
NcoI C'CATG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 3355
Fragments arranged by size:
3355 1239 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5146
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5146
NotI GC'GGCC_GC
Cuts at: 2593 2593
Size: 5146
NspI r_CATG'y
Cuts at: 1132 1229 2504 4049 1132
Size: 97 1275 1545 2229
Fragments arranged by size:
2229 1545 1275 97
PasI CC'CwG_GG
Cuts at: 1348 2139 2403 1348
Size: 791 264 4091
Fragments arranged by size:
4091 791 264
PciI A'CATG_T
Cuts at: 4045 4045
Size: 5146
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5146
PmeI GTTT'AAAC
Cuts at: 2450 2450
Size: 5146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 4728 4760 3215
Size: 32 1481 32 3601
Fragments arranged by size:
3601 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5146
PsiI TTA'TAA
Cuts at: 2716 2716
Size: 5146
PstI C_TGCA'G
Cuts at: 830 2498 830
Size: 1668 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5146
SalI G'TCGA_C
Cuts at: 1936 2488 1936
Size: 552 4594
SbfI CC_TGCA'GG
Cuts at: 2498 2498
Size: 5146
ScaI AGT'ACT
Cuts at: 3394 5106 3394
Size: 1712 3434
SfcI C'TryA_G
Cuts at: 826 1045 2317 2494 3629 4310 4501 826
Size: 219 1272 177 1135 681 191 1471
Fragments arranged by size:
1471 1272 1135 681 219 191 177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5146
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5146
SmaI CCC'GGG
Cuts at: 1872 2473 1872
Size: 601 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 3250 4151 4413 820
Size: 197 926 477 830 901 262 1553
Fragments arranged by size:
1553 926 901 830 477 262 197
SnaBI TAC'GTA
Cuts at: 493 2937 493
Size: 2444 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5146
SphI G_CATG'C
Cuts at: 1132 2504 1132
Size: 1372 3774
SspI AAT'ATT
Cuts at: 5 52 3070 5
Size: 47 3018 2081
Fragments arranged by size:
3018 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5146
StyI C'CwwG_G
Cuts at: 513 1065 2304 2585 513
Size: 552 1239 281 3074
Fragments arranged by size:
3074 1239 552 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3173 3190 3343 3528 3973 325
Size: 1255 1593 17 153 185 445 1498
Fragments arranged by size:
1593 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 3392 5104
Size: 274 80 33 51 1443 372 1043 1712
Cuts at: 5104 96
Size: 138
Fragments arranged by size:
1712 1443 1043 372 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2007 2039 5106 5138 2007
Size: 32 3067 32 2015
Fragments arranged by size:
3067 2015 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5146
XbaI T'CTAG_A
Cuts at: 2482 2482
Size: 5146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2375 2375
Size: 5146
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5146
XmaI C'CCGG_G
Cuts at: 1870 2471 1870
Size: 601 4545
XmnI GAAnn'nnTTC
Cuts at: 3275 3275
Size: 5146
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2954 276
Size: 53 83 186 936 1420 2468
Fragments arranged by size:
2468 1420 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI