Restriction map for
pFN21AE5792
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
KspI MluI MroI MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacI SacII SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 4 GCGG CCGC(-3/-1)
AclI 334 AACGTT AA^CGTT
AflIII 1531 ACATGT A^C[GA][CT]GT
AlwI 237 GATCC GGATC(4/5)
AlwNI 229 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 350 GTGCTC G[AT]GC[AT]^C
AvaI 1677 CTCGGG C^[CT]CG[GA]G
AvaII 231 GGACC G^G[AT]CC
BanII 1649 GGGCTC G[GA]GC[CT]^C
BcgI 453 CGAAATGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 1439 CTCCAG CTGGAG(16/14)
BsiHKAI 350 GTGCTC G[AT]GC[AT]^C
BsmAI 1097 GTCTC GTCTC(1/5)
BsmFI 43 GGGAC GGGAC(10/14)
BsoBI 1677 CTCGGG C^[CT]CG[GA]G
BspLU11I 1531 ACATGT A^CATGT
BsrDI 1030 GCAATG GCAATG(2/0)
Bst1107I 1596 GTATAC GTA^TAC
BstZ17I 1596 GTATAC GTA^TAC
DraI 775 TTTAAA TTT^AAA
DraII 230 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 869 CCATGG C^C[GA][CT]GG
EcoNI 1320 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 230 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1813 GAATTC G^AATTC
EcoT22I 1500 ATGCAT ATGCA^T
HincII 1231 GTCGAC GT[CT]^[GA]AC
HindII 1231 GTCGAC GT[CT]^[GA]AC
MaeII 335 ACGT A^CGT
MfeI 1025 CAATTG C^AATTG
MslI 928 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 71 CAGCTG C[AC]G^C[GT]G
MunI 1025 CAATTG C^AATTG
MwoI 1347 GCAGTATAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 869 CCATGG C^CATGG
NsiI 1500 ATGCAT ATGCA^T
PleI 1640 GACTC GAGTC(4/5)
PpuMI 230 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 334 AACGTT AA^CGTT
PstI 355 CTGCAG CTGCA^G
PvuII 71 CAGCTG CAG^CTG
SalI 1231 GTCGAC G^TCGAC
SfaNI 885 GATGC GCATC(5/9)
SmlI 719 CTCAAG C^T[CT][GA]AG
SphI 1761 GCATGC GCATG^C
TaqI 1232 TCGA T^CGA
TthHB8I 1232 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1231 GTCGAC GT^[AC][GT]AC
1596 GTATAC
BalI 1140 TGGCCA TGG^CCA
1904 TGGCCA
BglII 1358 AGATCT A^GATCT
1796 AGATCT
BmyI 350 GTGCTC G[AGT]GC[ACT]^C
1649 GGGCTC
BsaBI 161 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1405 GATTTAGATC
Bsp1286I 350 GTGCTC G[AGT]GC[ACT]^C
1649 GGGCTC
BstYI 1358 AGATCT [GA]^GATC[CT]
1796 AGATCT
Cfr13I 231 GGACC G^G[ACGT]CC
1735 GGGCC
EaeI 1140 TGGCCA [CT]^GGCC[GA]
1904 TGGCCA
EarI 263 CTCTTC CTCTTC(1/4)
340 GAAGAG
EcoT14I 869 CCATGG C^C[AT][AT]GG
1143 CCAAGG
HindIII 299 AAGCTT A^AGCTT
944 AAGCTT
Ksp632I 263 CTCTTC CTCTTC(1/4)
340 GAAGAG
MamI 161 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1405 GATTTAGATC
MflI 1358 AGATCT [GA]^GATC[CT]
1796 AGATCT
MluNI 1140 TGGCCA TGG^CCA
1904 TGGCCA
MscI 1140 TGGCCA TGG^CCA
1904 TGGCCA
NlaIV 1878 GGCTCC GG[ACGT]^[ACGT]CC
1885 GGAACC
Sau96I 231 GGACC G^G[ACGT]CC
1735 GGGCC
ScaI 214 AGTACT AGT^ACT
1371 AGTACT
SspI 688 AATATT AAT^ATT
1295 AATATT
StyI 869 CCATGG C^C[AT][AT]GG
1143 CCAAGG
Tsp45I 784 GTCAC ^GT[GC]AC
1471 GTCAC
XcmI 314 CCAGTCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1636 CCAAGACTCTTGTGG
XhoII 1358 AGATCT [GA]^GATC[CT]
1796 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BbsI BbvI BfaI BpuAI BsaJI
BsgI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NspI RsaI Sau3AI ScrFI
SfcI TfiI Tru9I TseI Tsp509I
TspRI XmnI