(Circular) (Six-base) MAPSORT of: pFN21AE5792.seq Check: 8360 from: 1 to: 6673 pFN21AE5792 6673 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4483 278 Size: 53 83 186 936 2947 2468 Fragments arranged by size: 2947 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3237 3602 4016 1937 Size: 1300 365 414 4594 Fragments arranged by size: 4594 1300 414 365 Acc65I G'GTAC_C Cuts at: 3994 3994 Size: 6673 AclI AA'CG_TT Cuts at: 1563 2340 4800 5173 1563 Size: 777 2460 373 3063 Fragments arranged by size: 3063 2460 777 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2421 2445 3080 3834 4736 6120 6388 1632 Size: 789 24 635 754 902 1384 268 1917 Fragments arranged by size: 1917 1384 902 789 754 635 268 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6476 AflIII A'CryG_T Cuts at: 1203 3536 5572 6549 1203 Size: 2333 2036 977 1327 Fragments arranged by size: 2333 2036 1327 977 AgeI A'CCGG_T Cuts at: 5504 5504 Size: 6673 AhdI GACnn_n'nnGTC Cuts at: 5402 5402 Size: 6673 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6673 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3048 1184 Size: 1864 4809 AlwNI CAG_nnn'CTG Cuts at: 2239 4416 5988 2239 Size: 2177 1572 2924 Fragments arranged by size: 2924 2177 1572 ApaLI G'TGCA_C Cuts at: 4730 5886 4730 Size: 1156 5517 ApoI r'AATT_y Cuts at: 1493 2777 2900 3156 3818 3982 4208 4360 6469 Size: 1284 123 256 662 164 226 152 2109 Cuts at: 6469 1493 Size: 1697 Fragments arranged by size: 2109 1697 1284 662 256 226 164 152 123 AseI AT'TA_AT Cuts at: 160 1032 5227 160 Size: 872 4195 1606 Fragments arranged by size: 4195 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6673 AvaI C'yCGr_G Cuts at: 1870 1943 3682 3998 1870 Size: 73 1739 316 4545 Fragments arranged by size: 4545 1739 316 73 BamHI G'GATC_C Cuts at: 4003 4370 4003 Size: 367 6306 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3994 5449 618 Size: 325 217 627 2207 1455 1842 Fragments arranged by size: 2207 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3658 3992 1391 Size: 2267 334 4072 Fragments arranged by size: 4072 2267 334 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3200 3321 3570 3852 928 Size: 971 1301 121 249 282 3749 Fragments arranged by size: 3749 1301 971 282 249 121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2447 2481 4864 4898 1903 Size: 34 510 34 2383 34 3678 Fragments arranged by size: 3678 2383 510 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4565 5775 4565 Size: 1210 5463 BclI T'GATC_A Cuts at: 1548 1548 Size: 6673 BfrBI ATG'CAT Cuts at: 3507 3507 Size: 6673 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5284 136 Size: 107 122 71 631 605 3612 1525 Fragments arranged by size: 3612 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3363 3801 6668 3363 Size: 438 2867 3368 Fragments arranged by size: 3368 2867 438 BlpI GC'TnA_GC Cuts at: 4090 4090 Size: 6673 Bme1580I G_kGCm'C Cuts at: 1790 4734 5890 1790 Size: 2944 1156 2573 Fragments arranged by size: 2944 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6673 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2152 4404 5362 Size: 416 53 83 399 24 735 2252 958 Cuts at: 5362 442 Size: 1753 Fragments arranged by size: 2252 1753 958 735 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6673 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3429 5333 1239 Size: 2190 1904 2579 Fragments arranged by size: 2579 2190 1904 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2709 4798 5663 5961 2709 Size: 2089 865 298 3421 Fragments arranged by size: 3421 2089 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5336 882 Size: 617 3837 2219 Fragments arranged by size: 3837 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4464 493 Size: 711 3260 2702 Fragments arranged by size: 3260 2702 711 BsaBI GATnn'nnATC Cuts at: 2170 3414 6667 2170 Size: 1244 3253 2176 Fragments arranged by size: 3253 2176 1244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4480 4862 5497 275 Size: 53 83 186 936 2947 382 635 1451 Fragments arranged by size: 2947 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5106 5504 5778 5925 6336 5106 Size: 398 274 147 411 5443 Fragments arranged by size: 5443 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6448 Fragments arranged by size: 6448 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6673 BseYI C'CCAG_C Cuts at: 5876 6372 5876 Size: 496 6177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3008 3204 3371 1163 Size: 573 1272 196 167 4465 Fragments arranged by size: 4465 1272 573 196 167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4123 4884 5912 1540 Size: 275 186 2122 761 1028 2301 Fragments arranged by size: 2301 2122 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2359 4734 4819 5890 2359 Size: 2375 85 1071 3142 Fragments arranged by size: 3142 2375 1071 85 BsmI GAATG_Cn' Cuts at: 4184 4277 4184 Size: 93 6580 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2359 3658 3992 4734 4819 5890 1391 Size: 399 569 1299 334 742 85 1071 2174 Fragments arranged by size: 2174 1299 1071 742 569 399 334 85 BspEI T'CCGG_A Cuts at: 6336 6336 Size: 6673 BspHI T'CATG_A Cuts at: 4562 6292 4562 Size: 1730 4943 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4028 844 Size: 3184 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4560 1394 Size: 429 2737 3507 Fragments arranged by size: 3507 2737 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3042 5168 5342 65 Size: 1183 1794 2126 174 1396 Fragments arranged by size: 2126 1794 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5317 5504 1700 Size: 110 3507 187 2869 Fragments arranged by size: 3507 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6673 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6673 BssSI C'ACGA_G Cuts at: 4733 5745 4733 Size: 1012 5661 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6673 BstBI TT'CG_AA Cuts at: 5477 5477 Size: 6673 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6673 BstYI r'GATC_y Cuts at: 1972 3363 3801 4003 4370 4755 4772 6224 6310 Size: 1391 438 202 367 385 17 1452 86 Cuts at: 6310 6322 6668 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1391 438 385 367 346 202 86 17 12 BstZ17I GTA'TAC Cuts at: 3603 3603 Size: 6673 BtgI C'CryG_G Cuts at: 513 1065 2004 2874 513 Size: 552 939 870 4312 Fragments arranged by size: 4312 939 870 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5934 Fragments arranged by size: 5934 683 56 BtsI GCAGTG_nn' Cuts at: 2966 4189 4983 5003 2966 Size: 1223 794 20 4636 Fragments arranged by size: 4636 1223 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6673 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2011 2046 529 Size: 35 1447 35 5156 Fragments arranged by size: 5156 1447 35 35 DraI TTT'AAA Cuts at: 2782 3977 2782 Size: 1195 5478 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5680 809 Size: 507 4364 1802 Fragments arranged by size: 4364 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3145 3909 4120 Size: 54 1425 50 275 18 1315 764 211 Cuts at: 4120 5009 6638 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1315 889 764 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4120 1537 Size: 275 2308 4090 Fragments arranged by size: 4090 2308 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2274 2340 4603 1353 Size: 921 66 2263 3423 Fragments arranged by size: 3423 2263 921 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5260 5634 5780 387 Size: 1064 138 435 3236 374 146 1280 Fragments arranged by size: 3236 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2421 2445 3080 3429 3834 4736 5333 Size: 393 789 24 635 349 405 902 597 Cuts at: 5333 6120 6388 1239 Size: 787 268 1524 Fragments arranged by size: 1524 902 789 787 635 597 405 393 349 268 24 EcoNI CCTnn'n_nnAGG Cuts at: 3329 3329 Size: 6673 EcoO109I rG'GnC_Cy Cuts at: 2236 2236 Size: 6673 EcoRI G'AATT_C Cuts at: 3818 3982 4360 3818 Size: 164 378 6131 Fragments arranged by size: 6131 378 164 FspI TGC'GCA Cuts at: 5179 5179 Size: 6673 HaeII r_GCGC'y Cuts at: 1678 5820 1678 Size: 4142 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2341 2373 2690 2722 2835 Size: 32 135 32 769 32 317 32 113 Cuts at: 2835 2867 3230 3262 3427 3459 3557 3589 5314 Size: 32 363 32 165 32 98 32 1725 Cuts at: 5314 5346 5388 5420 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1725 769 363 317 165 135 113 98 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3238 4017 4263 669 Size: 988 281 1300 779 246 3079 Fragments arranged by size: 3079 1300 988 779 281 246 HindIII A'AGCT_T Cuts at: 748 2304 2949 748 Size: 1556 645 4472 Fragments arranged by size: 4472 1556 645 HpaI GTT'AAC Cuts at: 4263 4263 Size: 6673 KpnI G_GTAC'C Cuts at: 3998 3998 Size: 6673 MfeI C'AATT_G Cuts at: 3030 4272 3030 Size: 1242 5431 MluI A'CGCG_T Cuts at: 6549 6549 Size: 6673 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3833 5787 5971 1305 Size: 2528 1954 184 2007 Fragments arranged by size: 2528 2007 1954 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3147 3911 10 Size: 54 1425 343 1315 764 2772 Fragments arranged by size: 2772 1425 1315 764 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2937 4631 4990 5149 518 Size: 648 1771 1694 359 159 2042 Fragments arranged by size: 2042 1771 1694 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2078 4089 4766 5914 6159 1606 Size: 472 2011 677 1148 245 2120 Fragments arranged by size: 2120 2011 1148 677 472 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2874 513 Size: 552 939 870 4312 Fragments arranged by size: 4312 939 870 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6673 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6673 NotI GC'GGCC_GC Cuts at: 4120 4120 Size: 6673 NsiI A_TGCA'T Cuts at: 3509 3509 Size: 6673 NspI r_CATG'y Cuts at: 1132 1229 2085 3342 3507 3540 3770 4031 5576 Size: 97 856 1257 165 33 230 261 1545 Cuts at: 5576 1132 Size: 2229 Fragments arranged by size: 2229 1545 1257 856 261 230 165 97 33 PasI CC'CwG_GG Cuts at: 1348 3163 1348 Size: 1815 4858 PciI A'CATG_T Cuts at: 3536 5572 3536 Size: 2036 4637 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6673 PfoI T'CCnGG_A Cuts at: 3795 3886 3795 Size: 91 6582 PmeI GTTT'AAAC Cuts at: 3977 3977 Size: 6673 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4742 4774 6255 6287 4742 Size: 32 1481 32 5128 Fragments arranged by size: 5128 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2236 2236 Size: 6673 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6673 PsiI TTA'TAA Cuts at: 2042 4243 2042 Size: 2201 4472 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2814 2846 2814 Size: 32 6641 PstI C_TGCA'G Cuts at: 830 2364 4025 830 Size: 1534 1661 3478 Fragments arranged by size: 3478 1661 1534 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6673 PvuII CAG'CTG Cuts at: 2078 2078 Size: 6673 SalI G'TCGA_C Cuts at: 1936 3236 4015 1936 Size: 1300 779 4594 Fragments arranged by size: 4594 1300 779 SbfI CC_TGCA'GG Cuts at: 4025 4025 Size: 6673 ScaI AGT'ACT Cuts at: 2221 3378 4921 6633 2221 Size: 1157 1543 1712 2261 Fragments arranged by size: 2261 1712 1543 1157 SfcI C'TryA_G Cuts at: 826 1045 2360 2613 3172 4021 5156 5837 6028 Size: 219 1315 253 559 849 1135 681 191 Cuts at: 6028 826 Size: 1471 Fragments arranged by size: 1471 1315 1135 849 681 559 253 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6673 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6673 SmaI CCC'GGG Cuts at: 1872 4000 1872 Size: 2128 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2724 4777 5678 5940 820 Size: 197 926 781 2053 901 262 1553 Fragments arranged by size: 2053 1553 926 901 781 262 197 SnaBI TAC'GTA Cuts at: 493 4464 493 Size: 3971 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6673 SphI G_CATG'C Cuts at: 1132 3770 4031 1132 Size: 2638 261 3774 Fragments arranged by size: 3774 2638 261 SspI AAT'ATT Cuts at: 5 52 2695 3302 4597 5 Size: 47 2643 607 1295 2081 Fragments arranged by size: 2643 2081 1295 607 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6673 StyI C'CwwG_G Cuts at: 513 1065 2004 2874 3148 4112 513 Size: 552 939 870 274 964 3074 Fragments arranged by size: 3074 964 939 870 552 274 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4700 4717 4870 5055 5500 325 Size: 1255 3120 17 153 185 445 1498 Fragments arranged by size: 3120 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2219 3019 3220 Size: 274 80 33 51 1443 242 800 201 Cuts at: 3220 3376 4919 6631 96 Size: 156 1543 1712 138 Fragments arranged by size: 1712 1543 1443 800 274 242 201 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6633 6665 6633 Size: 32 6641 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6673 XbaI T'CTAG_A Cuts at: 4009 4009 Size: 6673 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2326 3648 2326 Size: 1322 5351 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6673 XmaI C'CCGG_G Cuts at: 1870 3998 1870 Size: 2128 4545 XmnI GAAnn'nnTTC Cuts at: 2289 2829 3590 4802 2289 Size: 540 761 1212 4160 Fragments arranged by size: 4160 1212 761 540 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4481 276 Size: 53 83 186 936 2947 2468 Fragments arranged by size: 2947 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI