(Linear) (Six-base) MAPSORT of: KIEE5792.seq Check: 3891 from: 1 to: 1968 KIEE5792.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AccI GT'mk_AC Cuts at: 0 1232 1597 1968 Size: 1232 365 371 Fragments arranged by size: 1232 371 365 AclI AA'CG_TT Cuts at: 0 335 1968 Size: 335 1633 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 416 440 1075 1829 1968 Size: 416 24 635 754 139 Fragments arranged by size: 754 635 416 139 24 AflIII A'CryG_T Cuts at: 0 1531 1968 Size: 1531 437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1043 1968 Size: 1043 925 AlwNI CAG_nnn'CTG Cuts at: 0 234 1968 Size: 234 1734 ApoI r'AATT_y Cuts at: 0 772 895 1151 1813 1968 Size: 772 123 256 662 155 Fragments arranged by size: 772 662 256 155 123 AvaI C'yCGr_G Cuts at: 0 1677 1968 Size: 1677 291 BanII G_rGCy'C Cuts at: 0 1653 1968 Size: 1653 315 BbsI GAAGACnn'nnnn_ Cuts at: 0 1195 1316 1565 1847 1968 Size: 1195 121 249 282 121 Fragments arranged by size: 1195 282 249 121 121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 442 476 1968 Size: 442 34 1492 Fragments arranged by size: 1492 442 34 BfrBI ATG'CAT Cuts at: 0 1502 1968 Size: 1502 466 BglII A'GATC_T Cuts at: 0 1358 1796 1968 Size: 1358 438 172 Fragments arranged by size: 1358 438 172 BmrI ACTGGGnnnn_n' Cuts at: 0 147 1968 Size: 147 1821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1424 1968 Size: 1424 544 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 704 1968 Size: 704 1264 BsaBI GATnn'nnATC Cuts at: 0 165 1409 1968 Size: 165 1244 559 Fragments arranged by size: 1244 559 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1003 1199 1366 1968 Size: 1003 196 167 602 Fragments arranged by size: 1003 602 196 167 BsiHKAI G_wGCw'C Cuts at: 0 354 1968 Size: 354 1614 Bsp1286I G_dGCh'C Cuts at: 0 354 1653 1968 Size: 354 1299 315 Fragments arranged by size: 1299 354 315 BsrDI GCAATG_nn' Cuts at: 0 1037 1968 Size: 1037 931 BstYI r'GATC_y Cuts at: 0 1358 1796 1968 Size: 1358 438 172 Fragments arranged by size: 1358 438 172 BstZ17I GTA'TAC Cuts at: 0 1598 1968 Size: 1598 370 BtgI C'CryG_G Cuts at: 0 869 1968 Size: 869 1099 BtsI GCAGTG_nn' Cuts at: 0 961 1968 Size: 961 1007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 6 41 1968 Size: 6 35 1927 Fragments arranged by size: 1927 35 6 DraI TTT'AAA Cuts at: 0 777 1968 Size: 777 1191 EaeI y'GGCC_r Cuts at: 0 1140 1904 1968 Size: 1140 764 64 Fragments arranged by size: 1140 764 64 EarI CTCTTCn'nnn_ Cuts at: 0 269 335 1968 Size: 269 66 1633 Fragments arranged by size: 1633 269 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 1968 Size: 19 1949 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 416 440 1075 1424 1829 1968 Size: 416 24 635 349 405 139 Fragments arranged by size: 635 416 405 349 139 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 1324 1968 Size: 1324 644 EcoO109I rG'GnC_Cy Cuts at: 0 231 1968 Size: 231 1737 EcoRI G'AATT_C Cuts at: 0 1813 1968 Size: 1813 155 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 336 368 685 717 830 862 1225 1257 Size: 336 32 317 32 113 32 363 32 Cuts at: 1257 1422 1454 1552 1584 1968 Size: 165 32 98 32 384 Fragments arranged by size: 384 363 336 317 165 113 98 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1233 1968 Size: 1233 735 HindIII A'AGCT_T Cuts at: 0 299 944 1968 Size: 299 645 1024 Fragments arranged by size: 1024 645 299 MfeI C'AATT_G Cuts at: 0 1025 1968 Size: 1025 943 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1828 1968 Size: 1828 140 MscI TGG'CCA Cuts at: 0 1142 1906 1968 Size: 1142 764 62 Fragments arranged by size: 1142 764 62 MslI CAynn'nnrTG Cuts at: 0 932 1968 Size: 932 1036 MspA1I CmG'CkG Cuts at: 0 73 1968 Size: 73 1895 NcoI C'CATG_G Cuts at: 0 869 1968 Size: 869 1099 NsiI A_TGCA'T Cuts at: 0 1504 1968 Size: 1504 464 NspI r_CATG'y Cuts at: 0 80 1337 1502 1535 1765 1968 Size: 80 1257 165 33 230 203 Fragments arranged by size: 1257 230 203 165 80 33 PasI CC'CwG_GG Cuts at: 0 1158 1968 Size: 1158 810 PciI A'CATG_T Cuts at: 0 1531 1968 Size: 1531 437 PfoI T'CCnGG_A Cuts at: 0 1790 1881 1968 Size: 1790 91 87 Fragments arranged by size: 1790 91 87 PpuMI rG'GwC_Cy Cuts at: 0 231 1968 Size: 231 1737 PsiI TTA'TAA Cuts at: 0 37 1968 Size: 37 1931 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 809 841 1968 Size: 809 32 1127 Fragments arranged by size: 1127 809 32 PstI C_TGCA'G Cuts at: 0 359 1968 Size: 359 1609 PvuII CAG'CTG Cuts at: 0 73 1968 Size: 73 1895 SalI G'TCGA_C Cuts at: 0 1231 1968 Size: 1231 737 ScaI AGT'ACT Cuts at: 0 216 1373 1968 Size: 216 1157 595 Fragments arranged by size: 1157 595 216 SfcI C'TryA_G Cuts at: 0 355 608 1167 1968 Size: 355 253 559 801 Fragments arranged by size: 801 559 355 253 SmlI C'TyrA_G Cuts at: 0 719 1968 Size: 719 1249 SphI G_CATG'C Cuts at: 0 1765 1968 Size: 1765 203 SspI AAT'ATT Cuts at: 0 690 1297 1968 Size: 690 607 671 Fragments arranged by size: 690 671 607 StyI C'CwwG_G Cuts at: 0 869 1143 1968 Size: 869 274 825 Fragments arranged by size: 869 825 274 TatI w'GTAC_w Cuts at: 0 214 1014 1215 1371 1968 Size: 214 800 201 156 597 Fragments arranged by size: 800 597 214 201 156 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 321 1643 1968 Size: 321 1322 325 Fragments arranged by size: 1322 325 321 XmnI GAAnn'nnTTC Cuts at: 0 284 824 1585 1968 Size: 284 540 761 383 Fragments arranged by size: 761 540 383 284 Enzymes that do cut: AccI AclI AcuI AflIII AlfI AlwNI ApoI AvaI BanII BbsI BcgI BfrBI BglII BmrI BpmI BpuEI BsaBI BsgI BsiHKAI Bsp1286I BsrDI BstYI BstZ17I BtgI BtsI CspCI DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PfoI PpuMI PsiI PsrI PstI PvuII SalI ScaI SfcI SmlI SphI SspI StyI TatI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI ClaI DraIII DrdI EagI EcoICRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PvuI RsrII SacI SacII SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI