Restriction map for
pFN21AE3035
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmAI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI Ksp632I MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 82 GGCGCC G[GA]^CG[CT]C
AfaI 198 GTAC GT^AC
AlwNI 226 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 400 GAGCTC G[AT]GC[AT]^C
AvaI 403 CTCGAG C^[CT]CG[GA]G
AvaII 420 GGACC G^G[AT]CC
BanI 82 GGCGCC G^G[CT][GA]CC
BanII 400 GAGCTC G[GA]GC[CT]^C
BbeI 82 GGCGCC GGCGC^C
BbrPI 325 CACGTG CAC^GTG
BglI 228 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 162 CTCCAG CTGGAG(16/14)
BsaAI 325 CACGTG [CT]AC^GT[GA]
BsaHI 82 GGCGCC G[GA]^CG[CT]C
BsiEI 73 CGGCCG CG[GA][CT]^CG
BsiHKAI 400 GAGCTC G[AT]GC[AT]^C
BsoBI 403 CTCGAG C^[CT]CG[GA]G
BssSI 394 CTCGTG CACGAG(-5/-1)
BstAPI 225 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 183 GGTCACC G^GT[ACGT]ACC
BstPI 183 GGTCACC G^GT[ACGT]ACC
DdeI 484 CTGAG C^T[ACGT]AG
DpnII 144 GATC ^GATC
DraII 173 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 319 CCGCGG C^C[GA][CT]GG
EagI 73 CGGCCG C^GGCCG
EclXI 73 CGGCCG C^GGCCG
Eco52I 73 CGGCCG C^GGCCG
EcoO109I 173 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 183 GGTCACC G^GT[ACGT]ACC
HaeII 82 GGCGCC [GA]GCGC^[CT]
Hin1I 82 GGCGCC G[GA]^CG[CT]C
HindIII 10 AAGCTT A^AGCTT
KasI 82 GGCGCC G^GCGCC
KspI 319 CCGCGG CCGC^GG
MaeII 326 ACGT A^CGT
MboI 144 GATC ^GATC
MslI 471 CATGTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 75 GCCGGC GCC^GGC
NarI 82 GGCGCC GG^CGCC
NdeII 144 GATC ^GATC
NgoMIV 75 GCCGGC G^CCGGC
NlaIII 471 CATG CATG^
NspI 470 GCATGT [GA]CATG^[CT]
PaeR7I 403 CTCGAG C^TCGAG
PmaCI 325 CACGTG CAC^GTG
PmlI 325 CACGTG CAC^GTG
RsaI 198 GTAC GT^AC
SacI 400 GAGCTC GAGCT^C
SacII 319 CCGCGG CCGC^GG
Sau3AI 144 GATC ^GATC
SfoI 82 GGCGCC GGC^GCC
TaqI 404 TCGA T^CGA
TthHB8I 404 TCGA T^CGA
XhoI 403 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 320 CGCG CG^CG
379 CGCG
AluI 11 AGCT AG^CT
401 AGCT
BcnI 62 CCCGG CC^[GC]GG
412 CCCGG
BmyI 400 GAGCTC G[AGT]GC[ACT]^C
408 GTGCCC
Bsp1286I 400 GAGCTC G[AGT]GC[ACT]^C
408 GTGCCC
BstUI 320 CGCG CG^CG
379 CGCG
Cfr13I 174 GGCCC G^G[ACGT]CC
420 GGACC
EaeI 73 CGGCCG [CT]^GGCC[GA]
294 CGGCCA
FauI 318 CCCGC CCCGC(4/6)
79 GCGGG
HgaI 222 GACGC GACGC(5/10)
380 GCGTC
MboII 66 GAAGA GAAGA(8/7)
330 GAAGA
MspA1I 229 CCGCTG C[AC]G^C[GT]G
319 CCGCGG
MvnI 320 CGCG CG^CG
379 CGCG
NciI 62 CCCGG CC^[GC]GG
412 CCCGG
Sau96I 174 GGCCC G^G[ACGT]CC
420 GGACC
TspRI 298 CAGTG CA[GC]TG[ACGT][ACGT]^
460 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI BbvI BsaJI BseRI BsiYI
BslI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MnlI MspI MvaI MwoI
NlaIV PleI ScrFI SmlI TseI
Tsp45I