(Circular) (Six-base) MAPSORT of: pFN21AE3035.seq Check: 9472 from: 1 to: 5209 pFN21AE3035 5209 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3019 278 Size: 53 83 186 936 1483 2468 Fragments arranged by size: 2468 1483 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2552 1937 Size: 615 4594 Acc65I G'GTAC_C Cuts at: 2530 2530 Size: 5209 AclI AA'CG_TT Cuts at: 1563 3336 3709 1563 Size: 1773 373 3063 Fragments arranged by size: 3063 1773 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3272 4656 4924 1632 Size: 1640 1384 268 1917 Fragments arranged by size: 1917 1640 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5012 AflIII A'CryG_T Cuts at: 1203 4108 5085 1203 Size: 2905 977 1327 Fragments arranged by size: 2905 1327 977 AgeI A'CCGG_T Cuts at: 4040 4040 Size: 5209 AhdI GACnn_n'nnGTC Cuts at: 3938 3938 Size: 5209 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5209 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5209 AlwNI CAG_nnn'CTG Cuts at: 2236 2952 4524 2236 Size: 716 1572 2921 Fragments arranged by size: 2921 1572 716 ApaLI G'TGCA_C Cuts at: 3266 4422 3266 Size: 1156 4053 ApoI r'AATT_y Cuts at: 1493 2518 2744 2896 5005 1493 Size: 1025 226 152 2109 1697 Fragments arranged by size: 2109 1697 1025 226 152 AseI AT'TA_AT Cuts at: 160 1032 3763 160 Size: 872 2731 1606 Fragments arranged by size: 2731 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5209 AvaI C'yCGr_G Cuts at: 1870 1943 2408 2534 1870 Size: 73 465 126 4545 Fragments arranged by size: 4545 465 126 73 BamHI G'GATC_C Cuts at: 2539 2906 2539 Size: 367 4842 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2530 3985 618 Size: 325 217 627 300 443 1455 1842 Fragments arranged by size: 1842 1455 627 443 325 300 217 BanII G_rGCy'C Cuts at: 1391 2409 2528 1391 Size: 1018 119 4072 Fragments arranged by size: 4072 1018 119 BbeI G_GCGC'C Cuts at: 2091 2091 Size: 5209 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3400 3434 1903 Size: 34 1463 34 3678 Fragments arranged by size: 3678 1463 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3101 4311 3101 Size: 1210 3999 BclI T'GATC_A Cuts at: 1548 1548 Size: 5209 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2239 3820 136 Size: 107 122 71 631 605 567 1581 1525 Fragments arranged by size: 1581 1525 631 605 567 122 107 71 BglII A'GATC_T Cuts at: 5204 5204 Size: 5209 BlpI GC'TnA_GC Cuts at: 2626 2626 Size: 5209 Bme1580I G_kGCm'C Cuts at: 1790 2417 3270 4426 1790 Size: 627 853 1156 2573 Fragments arranged by size: 2573 1156 853 627 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5209 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2940 3898 442 Size: 416 53 83 399 24 1523 958 1753 Fragments arranged by size: 1753 1523 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5209 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2152 3869 1239 Size: 913 1717 2579 Fragments arranged by size: 2579 1717 913 Bpu10I CC'TnA_GC Cuts at: 2489 2489 Size: 5209 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2192 2426 3334 4199 4497 2192 Size: 234 908 865 298 2904 Fragments arranged by size: 2904 908 865 298 234 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3872 882 Size: 617 2373 2219 Fragments arranged by size: 2373 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2332 3000 493 Size: 711 1128 668 2702 Fragments arranged by size: 2702 1128 711 668 BsaBI GATnn'nnATC Cuts at: 5203 5203 Size: 5209 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 3016 3398 4033 Size: 53 83 186 936 555 928 382 635 Cuts at: 4033 275 Size: 1451 Fragments arranged by size: 1451 936 928 635 555 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3642 4040 4314 4461 4872 3642 Size: 398 274 147 411 3979 Fragments arranged by size: 3979 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2333 2363 2396 2426 1178 Size: 30 165 30 930 30 33 30 3961 Fragments arranged by size: 3961 930 165 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2357 2360 1186 Size: 1084 87 3 4035 Fragments arranged by size: 4035 1084 87 3 BseYI C'CCAG_C Cuts at: 2379 4412 4908 2379 Size: 2033 496 2680 Fragments arranged by size: 2680 2033 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4636 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2081 2659 3420 4448 1540 Size: 275 186 80 578 761 1028 2301 Fragments arranged by size: 2301 1028 761 578 275 186 80 BsiHKAI G_wGCw'C Cuts at: 2409 3270 3355 4426 2409 Size: 861 85 1071 3192 Fragments arranged by size: 3192 1071 861 85 BsmI GAATG_Cn' Cuts at: 2720 2813 2720 Size: 93 5116 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2409 2417 2528 3270 3355 4426 1391 Size: 399 619 8 111 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 619 399 111 85 8 BspEI T'CCGG_A Cuts at: 4872 4872 Size: 5209 BspHI T'CATG_A Cuts at: 3098 4828 3098 Size: 1730 3479 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2497 2564 844 Size: 1653 67 3489 Fragments arranged by size: 3489 1653 67 BsrBI CCG'CTC Cuts at: 1394 1823 3096 1394 Size: 429 1273 3507 Fragments arranged by size: 3507 1273 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3704 3878 65 Size: 1183 2456 174 1396 Fragments arranged by size: 2456 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2076 2080 2469 3853 4040 1700 Size: 110 266 4 389 1384 187 2869 Fragments arranged by size: 2869 1384 389 266 187 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5209 BssSI C'ACGA_G Cuts at: 2399 3269 4281 2399 Size: 870 1012 3327 Fragments arranged by size: 3327 1012 870 BstAPI GCAn_nnn'nTGC Cuts at: 794 2236 794 Size: 1442 3767 BstBI TT'CG_AA Cuts at: 4013 4013 Size: 5209 BstEII G'GTnAC_C Cuts at: 1184 2188 1184 Size: 1004 4205 BstYI r'GATC_y Cuts at: 1972 2539 2906 3291 3308 4760 4846 4858 5204 Size: 567 367 385 17 1452 86 12 346 Cuts at: 5204 1972 Size: 1977 Fragments arranged by size: 1977 1452 567 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2324 513 Size: 552 1259 3398 Fragments arranged by size: 3398 1259 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4470 Fragments arranged by size: 4470 683 56 BtsI GCAGTG_nn' Cuts at: 2471 2725 3519 3539 2471 Size: 254 794 20 4141 Fragments arranged by size: 4141 794 254 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5209 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5174 DraI TTT'AAA Cuts at: 2513 2513 Size: 5209 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4216 809 Size: 507 2900 1802 Fragments arranged by size: 2900 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2078 2299 2656 Size: 54 1425 50 275 18 248 221 357 Cuts at: 2656 3545 5174 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 357 275 248 221 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2078 2656 1537 Size: 275 266 578 4090 Fragments arranged by size: 4090 578 275 266 EarI CTCTTCn'nnn_ Cuts at: 1353 3139 1353 Size: 1786 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2075 3796 4170 4316 387 Size: 1064 138 486 1721 374 146 1280 Fragments arranged by size: 1721 1280 1064 486 374 146 138 EcoICRI GAG'CTC Cuts at: 2407 2407 Size: 5209 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2152 3272 3869 4656 4924 1239 Size: 393 520 1120 597 787 268 1524 Fragments arranged by size: 1524 1120 787 597 520 393 268 EcoO109I rG'GnC_Cy Cuts at: 2179 2179 Size: 5209 EcoRI G'AATT_C Cuts at: 2518 2896 2518 Size: 378 4831 FspI TGC'GCA Cuts at: 3715 3715 Size: 5209 HaeII r_GCGC'y Cuts at: 1678 2091 4356 1678 Size: 413 2265 2531 Fragments arranged by size: 2531 2265 413 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2333 2365 3850 3882 3924 Size: 32 135 32 761 32 1485 32 42 Cuts at: 3924 3956 1373 Size: 32 2626 Fragments arranged by size: 2626 1485 761 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2553 2799 669 Size: 988 281 615 246 3079 Fragments arranged by size: 3079 988 615 281 246 HindIII A'AGCT_T Cuts at: 748 2015 748 Size: 1267 3942 HpaI GTT'AAC Cuts at: 2799 2799 Size: 5209 KasI G'GCGC_C Cuts at: 2087 2087 Size: 5209 KpnI G_GTAC'C Cuts at: 2534 2534 Size: 5209 MfeI C'AATT_G Cuts at: 2808 2808 Size: 5209 MluI A'CGCG_T Cuts at: 5085 5085 Size: 5209 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2461 4323 4507 1305 Size: 1156 1862 184 2007 Fragments arranged by size: 2007 1862 1156 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3387 Fragments arranged by size: 3387 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2480 3167 3526 3685 518 Size: 648 1314 687 359 159 2042 Fragments arranged by size: 2042 1314 687 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2236 2326 2625 3302 4450 4695 1606 Size: 630 90 299 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 630 299 245 90 NaeI GCC'GGC Cuts at: 2082 2082 Size: 5209 NarI GG'CG_CC Cuts at: 2088 2088 Size: 5209 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4657 NdeI CA'TA_TG Cuts at: 387 387 Size: 5209 NgoMIV G'CCGG_C Cuts at: 2080 2080 Size: 5209 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5209 NotI GC'GGCC_GC Cuts at: 2656 2656 Size: 5209 NspI r_CATG'y Cuts at: 1132 1229 2479 2567 4112 1132 Size: 97 1250 88 1545 2229 Fragments arranged by size: 2229 1545 1250 97 88 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5209 PciI A'CATG_T Cuts at: 4108 4108 Size: 5209 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4307 PmeI GTTT'AAAC Cuts at: 2513 2513 Size: 5209 PmlI CAC'GTG Cuts at: 2332 2332 Size: 5209 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3278 3310 4791 4823 3278 Size: 32 1481 32 3664 Fragments arranged by size: 3664 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5209 PsiI TTA'TAA Cuts at: 2779 2779 Size: 5209 PspXI vC'TCGA_Gb Cuts at: 2408 2408 Size: 5209 PstI C_TGCA'G Cuts at: 830 2561 830 Size: 1731 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5209 SacI G_AGCT'C Cuts at: 2409 2409 Size: 5209 SacII CC_GC'GG Cuts at: 2327 2327 Size: 5209 SalI G'TCGA_C Cuts at: 1936 2551 1936 Size: 615 4594 SbfI CC_TGCA'GG Cuts at: 2561 2561 Size: 5209 ScaI AGT'ACT Cuts at: 3457 5169 3457 Size: 1712 3497 SfcI C'TryA_G Cuts at: 826 1045 2557 3692 4373 4564 826 Size: 219 1512 1135 681 191 1471 Fragments arranged by size: 1512 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2089 2089 Size: 5209 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5209 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5209 SmaI CCC'GGG Cuts at: 1872 2536 1872 Size: 664 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2207 2408 2441 3313 4214 4476 Size: 197 926 264 201 33 872 901 262 Cuts at: 4476 820 Size: 1553 Fragments arranged by size: 1553 926 901 872 264 262 201 197 33 SnaBI TAC'GTA Cuts at: 493 3000 493 Size: 2507 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5209 SphI G_CATG'C Cuts at: 1132 2567 1132 Size: 1435 3774 SspI AAT'ATT Cuts at: 5 52 3133 5 Size: 47 3081 2081 Fragments arranged by size: 3081 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5209 StyI C'CwwG_G Cuts at: 513 1065 2648 513 Size: 552 1583 3074 Fragments arranged by size: 3074 1583 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3236 3253 3406 3591 4036 325 Size: 1255 1656 17 153 185 445 1498 Fragments arranged by size: 1656 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3455 5167 96 Size: 274 80 33 51 1443 1478 1712 138 Fragments arranged by size: 1712 1478 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2396 2428 5169 5201 2396 Size: 32 2741 32 2404 Fragments arranged by size: 2741 2404 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5209 XbaI T'CTAG_A Cuts at: 2545 2545 Size: 5209 XhoI C'TCGA_G Cuts at: 1943 2408 1943 Size: 465 4744 XmaI C'CCGG_G Cuts at: 1870 2534 1870 Size: 664 4545 XmnI GAAnn'nnTTC Cuts at: 3338 3338 Size: 5209 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3017 276 Size: 53 83 186 936 1483 2468 Fragments arranged by size: 2468 1483 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI