################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AhdI AlwNI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BciVI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI Eam1105I EarI Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeII MamI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1078 GTCTAC GT^[AC][GT]AC AcsI 541 GAATTC [GA]^AATT[CT] AcyI 699 GGCGCC G[GA]^CG[CT]C AflII 514 CTTAAG C^TTAAG AlwI 567 GGATC GGATC(4/5) ApoI 541 GAATTC [GA]^AATT[CT] BalI 410 TGGCCA TGG^CCA BbeI 699 GGCGCC GGCGC^C BclI 617 TGATCA T^GATCA BfaI 887 CTAG C^TAG BfrI 514 CTTAAG C^TTAAG BsaHI 699 GGCGCC G[GA]^CG[CT]C BsaI 634 GAGACC GGTCTC(1/5) BsrBI 193 GAGCGG CCGCTC(-3/-3) BssHII 22 GCGCGC G^CGCGC BssSI 601 CTCGTG CACGAG(-5/-1) BstAPI 1052 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 567 GGATCT [GA]^GATC[CT] EcoRI 541 GAATTC G^AATTC FbaI 617 TGATCA T^GATCA Hin1I 699 GGCGCC G[GA]^CG[CT]C HinfI 880 GACTC G^A[ACGT]TC KasI 699 GGCGCC G^GCGCC MaeI 887 CTAG C^TAG MaeIII 1003 GTGAC ^GT[ACGT]AC MboII 948 GAAGA GAAGA(8/7) MflI 567 GGATCT [GA]^GATC[CT] MluNI 410 TGGCCA TGG^CCA MscI 410 TGGCCA TGG^CCA MseI 515 TTAA T^TAA NaeI 781 GCCGGC GCC^GGC NarI 699 GGCGCC GG^CGCC NcoI 651 CCATGG C^CATGG NgoMIV 781 GCCGGC G^CCGGC NotI 148 GCGGCCGC GC^GGCCGC NspI 209 GCATGT [GA]CATG^[CT] PaeR7I 271 CTCGAG C^TCGAG PflMI 708 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 880 GACTC GAGTC(4/5) SfoI 699 GGCGCC GGC^GCC TaqI 272 TCGA T^CGA Tru9I 515 TTAA T^TAA Tsp45I 1003 GTGAC ^GT[GC]AC Tsp509I 542 AATT ^AATT TthHB8I 272 TCGA T^CGA Van91I 708 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 271 CTCGAG C^TCGAG XhoII 567 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AfaI 446 GTAC GT^AC 519 GTAC Aor51HI 299 AGCGCT AGC^GCT 440 AGCGCT BanII 109 GAGCCC G[GA]GC[CT]^C 575 GAGCCC BcgI 786 CGAGGCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 524 GCAGCAAGTTCG BseRI 384 CTCCTC GAGGAG(10/8) 1072 GAGGAG BsmBI 238 GAGACG CGTCTC(1/5) 742 GAGACG BsmFI 221 GGGAC GGGAC(10/14) 12 GTCCC BsrFI 179 ACCGGC [GA]^CCGG[CT] 781 GCCGGC Cfr10I 179 ACCGGC [GA]^CCGG[CT] 781 GCCGGC DpnII 568 GATC ^GATC 618 GATC EagI 80 CGGCCG C^GGCCG 149 CGGCCG EclXI 80 CGGCCG C^GGCCG 149 CGGCCG Eco47III 299 AGCGCT AGC^GCT 440 AGCGCT Eco52I 80 CGGCCG C^GGCCG 149 CGGCCG EcoT14I 413 CCAAGG C^C[AT][AT]GG 651 CCATGG HphI 615 GGTGA GGTGA(8/7) 1002 GGTGA KspI 35 CCGCGG CCGC^GG 975 CCGCGG MboI 568 GATC ^GATC 618 GATC NdeII 568 GATC ^GATC 618 GATC PpuMI 10 GGGTCCC [GA]G^G[AT]CC[CT] 221 GGGACCC RsaI 446 GTAC GT^AC 519 GTAC SacII 35 CCGCGG CCGC^GG 975 CCGCGG Sau3AI 568 GATC ^GATC 618 GATC SfaNI 723 GCATC GCATC(5/9) 962 GCATC SmaI 225 CCCGGG CCC^GGG 915 CCCGGG StuI 170 AGGCCT AGG^CCT 482 AGGCCT StyI 413 CCAAGG C^C[AT][AT]GG 651 CCATGG XmaCI 225 CCCGGG C^CCGGG 915 CCCGGG XmaI 225 CCCGGG C^CCGGG 915 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AccII AciI AluI Alw44I ApaLI AspHI AvaI AvaII BanI BbvI BcnI BglI BmyI BpmI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI SmlI TseI TspRI