(Circular) (Six-base) MAPSORT of: pFN21AE1848.seq Check: 4740 from: 1 to: 5800 pFN21AE1848 5800 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2654 2786 2825 3098 2654 Size: 132 39 273 5356 Fragments arranged by size: 5356 273 132 39 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3084 3143 1937 Size: 1147 59 4594 Fragments arranged by size: 4594 1147 59 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2753 3863 5247 5515 1632 Size: 578 543 1110 1384 268 1917 Fragments arranged by size: 1917 1384 1110 578 543 268 AfeI AGC'GCT Cuts at: 2306 2447 2306 Size: 141 5659 AflII C'TTAA_G Cuts at: 820 1017 2519 820 Size: 197 1502 4101 Fragments arranged by size: 4101 1502 197 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 2278 1166 Size: 1112 4688 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2296 2591 1184 Size: 1112 295 4393 Fragments arranged by size: 4393 1112 295 AlwNI CAG_nnn'CTG Cuts at: 3543 5115 3543 Size: 1572 4228 ApaLI G'TGCA_C Cuts at: 2271 2873 3064 3857 5013 2271 Size: 602 191 793 1156 3058 Fragments arranged by size: 3058 1156 793 602 191 ApoI r'AATT_y Cuts at: 1493 2546 3109 3335 3487 5596 1493 Size: 1053 563 226 152 2109 1697 Fragments arranged by size: 2109 1697 1053 563 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 2230 2276 2827 2920 3125 1870 Size: 73 287 46 551 93 205 4545 Fragments arranged by size: 4545 551 287 205 93 73 46 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2464 2704 2798 2924 2940 Size: 325 217 627 677 240 94 126 16 Cuts at: 2940 3121 4576 618 Size: 181 1455 1842 Fragments arranged by size: 1842 1455 677 627 325 240 217 181 126 94 16 BanII G_rGCy'C Cuts at: 1391 2118 2584 3119 1391 Size: 727 466 535 4072 Fragments arranged by size: 4072 727 535 466 BbeI G_GCGC'C Cuts at: 2708 2708 Size: 5800 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4829 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2518 2552 2780 2814 3991 Size: 34 50 34 497 34 228 34 1177 Cuts at: 3991 4025 1903 Size: 34 3678 Fragments arranged by size: 3678 1177 497 228 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 2622 1548 Size: 1074 4726 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2162 2792 2831 Size: 107 122 71 631 605 490 630 39 Cuts at: 2831 2907 4411 136 Size: 76 1504 1525 Fragments arranged by size: 1525 1504 631 630 605 490 122 107 76 71 39 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 3217 3217 Size: 5800 Bme1580I G_kGCm'C Cuts at: 1790 2275 2877 2927 3068 3861 5017 1790 Size: 485 602 50 141 793 1156 2573 Fragments arranged by size: 2573 1156 793 602 485 141 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3531 4489 442 Size: 416 53 83 399 24 2114 958 1753 Fragments arranged by size: 2114 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 2216 2342 2495 2847 4460 1239 Size: 923 54 126 153 352 1613 2579 Fragments arranged by size: 2579 1613 923 352 153 126 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 2741 3925 4790 5088 2333 Size: 408 1184 865 298 3045 Fragments arranged by size: 3045 1184 865 408 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2633 4463 882 Size: 617 1134 1830 2219 Fragments arranged by size: 2219 1830 1134 617 BsaAI yAC'GTr Cuts at: 493 1204 3591 493 Size: 711 2387 2702 Fragments arranged by size: 2702 2387 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2705 3607 3989 4624 Size: 53 83 186 936 1172 902 382 635 Cuts at: 4624 275 Size: 1451 Fragments arranged by size: 1451 1172 936 902 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5575 Fragments arranged by size: 5575 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2380 3092 1186 Size: 1194 712 3894 Fragments arranged by size: 3894 1194 712 BseYI C'CCAG_C Cuts at: 2630 5003 5499 2630 Size: 2373 496 2931 Fragments arranged by size: 2931 2373 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2299 2446 3076 1163 Size: 573 563 147 630 3887 Fragments arranged by size: 3887 630 573 563 147 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 2157 2185 3250 4011 5039 Size: 275 186 87 69 28 1065 761 1028 Cuts at: 5039 1540 Size: 2301 Fragments arranged by size: 2301 1065 1028 761 275 186 87 69 28 BsiHKAI G_wGCw'C Cuts at: 2275 2877 3068 3861 3946 5017 2275 Size: 602 191 793 85 1071 3058 Fragments arranged by size: 3058 1071 793 602 191 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 BsmBI CGTCTCn'nnnn_ Cuts at: 2237 2741 2237 Size: 504 5296 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2275 2584 2877 2927 3068 3119 Size: 399 328 157 309 293 50 141 51 Cuts at: 3119 3861 3946 5017 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 309 293 157 141 85 51 50 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2462 2654 2696 2786 2825 3098 3155 844 Size: 1618 192 42 90 39 273 57 3489 Fragments arranged by size: 3489 1618 273 192 90 57 42 39 BsrBI CCG'CTC Cuts at: 1394 1823 2200 3687 1394 Size: 429 377 1487 3507 Fragments arranged by size: 3507 1487 429 377 BsrDI GCAATG_nn' Cuts at: 65 1248 4295 4469 65 Size: 1183 3047 174 1396 Fragments arranged by size: 3047 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2184 2786 4444 4631 1700 Size: 110 374 602 1658 187 2869 Fragments arranged by size: 2869 1658 602 374 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 2027 1925 Size: 102 5698 BssSI C'ACGA_G Cuts at: 2606 3860 4872 2606 Size: 1254 1012 3534 Fragments arranged by size: 3534 1254 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3063 794 Size: 2269 3531 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstYI r'GATC_y Cuts at: 1972 2572 3130 3497 3882 3899 5351 5437 5449 Size: 600 558 367 385 17 1452 86 12 Cuts at: 5449 5795 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 600 558 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2040 2656 2813 2980 513 Size: 552 975 616 157 167 3333 Fragments arranged by size: 3333 975 616 552 167 157 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2472 487 Size: 683 56 1246 3815 Fragments arranged by size: 3815 1246 683 56 BtsI GCAGTG_nn' Cuts at: 2501 3068 3316 4110 4130 2501 Size: 567 248 794 20 4171 Fragments arranged by size: 4171 794 567 248 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2085 2154 Size: 54 1425 50 275 18 207 48 69 Cuts at: 2154 2415 2908 2932 2977 2983 3247 4136 5765 Size: 261 493 24 45 6 264 889 1629 Cuts at: 5765 8 Size: 43 Fragments arranged by size: 1629 1425 889 493 275 264 261 207 69 54 50 48 45 43 24 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2085 2154 3247 1537 Size: 275 273 69 1093 4090 Fragments arranged by size: 4090 1093 275 273 69 EarI CTCTTCn'nnn_ Cuts at: 1353 3730 1353 Size: 2377 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 4761 4907 387 Size: 1064 138 2798 374 146 1280 Fragments arranged by size: 2798 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2210 2216 2342 2495 2753 2847 Size: 393 530 48 6 126 153 258 94 Cuts at: 2847 3863 4460 5247 5515 1239 Size: 1016 597 787 268 1524 Fragments arranged by size: 1524 1016 787 597 530 393 268 258 153 126 94 48 6 EcoO109I rG'GnC_Cy Cuts at: 2016 2227 3015 2016 Size: 211 788 4801 Fragments arranged by size: 4801 788 211 EcoRI G'AATT_C Cuts at: 2546 3109 3487 2546 Size: 563 378 4859 Fragments arranged by size: 4859 563 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2374 2374 Size: 5800 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 2308 2449 2708 4947 1678 Size: 630 141 259 2239 2531 Fragments arranged by size: 2531 2239 630 259 141 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4441 4473 4515 4547 1373 Size: 32 135 32 2869 32 42 32 2626 Fragments arranged by size: 2869 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3144 3390 669 Size: 988 281 1206 246 3079 Fragments arranged by size: 3079 1206 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KasI G'GCGC_C Cuts at: 2704 2704 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4914 5098 1305 Size: 3609 184 2007 Fragments arranged by size: 3609 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2417 10 Size: 54 1425 343 585 3393 Fragments arranged by size: 3393 1425 585 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2278 2742 2880 3758 4117 4276 518 Size: 648 1112 464 138 878 359 159 2042 Fragments arranged by size: 2042 1112 878 648 464 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2023 2042 2134 2209 2851 2930 2982 3216 Size: 417 19 92 75 642 79 52 234 Cuts at: 3216 3893 5041 5286 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 642 417 245 234 92 79 75 52 19 NaeI GCC'GGC Cuts at: 2788 2788 Size: 5800 NarI GG'CG_CC Cuts at: 2705 2705 Size: 5800 NcoI C'CATG_G Cuts at: 513 1065 2656 513 Size: 552 1591 3657 Fragments arranged by size: 3657 1591 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NgoMIV G'CCGG_C Cuts at: 2786 2786 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 2154 3247 2154 Size: 1093 4707 NspI r_CATG'y Cuts at: 1132 1229 2218 3158 4703 1132 Size: 97 989 940 1545 2229 Fragments arranged by size: 2229 1545 989 940 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 2719 1108 Size: 1611 4189 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2270 2302 3869 3901 5382 5414 2270 Size: 32 1567 32 1481 32 2656 Fragments arranged by size: 2656 1567 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2016 2227 2016 Size: 211 5589 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PspXI vC'TCGA_Gb Cuts at: 2276 2276 Size: 5800 PstI C_TGCA'G Cuts at: 830 3152 830 Size: 2322 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 SacII CC_GC'GG Cuts at: 2043 2983 2043 Size: 940 4860 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SanDI GG'GwC_CC Cuts at: 2016 2227 2016 Size: 211 5589 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SfcI C'TryA_G Cuts at: 826 1045 3148 4283 4964 5155 826 Size: 219 2103 1135 681 191 1471 Fragments arranged by size: 2103 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2706 2706 Size: 5800 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 2232 2922 3127 1872 Size: 360 690 205 4545 Fragments arranged by size: 4545 690 360 205 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 2348 2519 2756 3904 4805 Size: 197 926 333 72 171 237 1148 901 Cuts at: 4805 5067 820 Size: 262 1553 Fragments arranged by size: 1553 1148 926 901 333 262 237 197 171 72 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2177 2489 1517 Size: 660 312 4828 Fragments arranged by size: 4828 660 312 StyI C'CwwG_G Cuts at: 513 1065 2418 2656 3239 513 Size: 552 1353 238 583 3074 Fragments arranged by size: 3074 1353 583 552 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2600 3827 3844 3997 4182 4627 325 Size: 1255 1020 1227 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1227 1020 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2523 4046 5758 Size: 274 80 33 51 1443 546 1523 1712 Cuts at: 5758 96 Size: 138 Fragments arranged by size: 1712 1523 1443 546 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5760 5792 5760 Size: 32 5768 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 2276 1943 Size: 333 5467 XmaI C'CCGG_G Cuts at: 1870 2230 2920 3125 1870 Size: 360 690 205 4545 Fragments arranged by size: 4545 690 360 205 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI