(Linear) (Six-base) MAPSORT of: KIEE1848.seq Check: 7028 from: 1 to: 1095 KIEE1848.orf With 182 enzymes: SgfI * July 15, 2010 18:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 649 781 820 1093 1095 Size: 649 132 39 273 2 Fragments arranged by size: 649 273 132 39 2 AccI GT'mk_AC Cuts at: 0 1079 1095 Size: 1079 16 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 748 1095 Size: 205 543 347 Fragments arranged by size: 543 347 205 AfeI AGC'GCT Cuts at: 0 301 442 1095 Size: 301 141 653 Fragments arranged by size: 653 301 141 AflII C'TTAA_G Cuts at: 0 514 1095 Size: 514 581 AleI CACnn'nnGTG Cuts at: 0 273 1095 Size: 273 822 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 291 586 1095 Size: 291 295 509 Fragments arranged by size: 509 295 291 ApaLI G'TGCA_C Cuts at: 0 266 868 1059 1095 Size: 266 602 191 36 Fragments arranged by size: 602 266 191 36 ApoI r'AATT_y Cuts at: 0 541 1095 Size: 541 554 AvaI C'yCGr_G Cuts at: 0 225 271 822 915 1095 Size: 225 46 551 93 180 Fragments arranged by size: 551 225 180 93 46 BanI G'GyrC_C Cuts at: 0 459 699 793 919 935 1095 Size: 459 240 94 126 16 160 Fragments arranged by size: 459 240 160 126 94 16 BanII G_rGCy'C Cuts at: 0 113 579 1095 Size: 113 466 516 Fragments arranged by size: 516 466 113 BbeI G_GCGC'C Cuts at: 0 703 1095 Size: 703 392 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 513 547 775 809 1095 Size: 513 34 228 34 286 Fragments arranged by size: 513 286 228 34 34 BclI T'GATC_A Cuts at: 0 617 1095 Size: 617 478 BglI GCCn_nnn'nGGC Cuts at: 0 157 787 826 902 1095 Size: 157 630 39 76 193 Fragments arranged by size: 630 193 157 76 39 Bme1580I G_kGCm'C Cuts at: 0 270 872 922 1063 1095 Size: 270 602 50 141 32 Fragments arranged by size: 602 270 141 50 32 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 211 337 490 842 1095 Size: 157 54 126 153 352 253 Fragments arranged by size: 352 253 157 153 126 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 736 1095 Size: 328 408 359 Fragments arranged by size: 408 359 328 BsaI GGTCTCn'nnnn_ Cuts at: 0 628 1095 Size: 628 467 BsaHI Gr'CG_yC Cuts at: 0 700 1095 Size: 700 395 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 375 1087 1095 Size: 375 712 8 Fragments arranged by size: 712 375 8 BseYI C'CCAG_C Cuts at: 0 625 1095 Size: 625 470 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 441 1071 1095 Size: 294 147 630 24 Fragments arranged by size: 630 294 147 24 BsiEI CG_ry'CG Cuts at: 0 83 152 180 1095 Size: 83 69 28 915 Fragments arranged by size: 915 83 69 28 BsiHKAI G_wGCw'C Cuts at: 0 270 872 1063 1095 Size: 270 602 191 32 Fragments arranged by size: 602 270 191 32 BsmBI CGTCTCn'nnnn_ Cuts at: 0 232 736 1095 Size: 232 504 359 Fragments arranged by size: 504 359 232 Bsp1286I G_dGCh'C Cuts at: 0 113 270 579 872 922 1063 1095 Size: 113 157 309 293 50 141 32 Fragments arranged by size: 309 293 157 141 113 50 32 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 457 649 691 781 820 1093 1095 Size: 457 192 42 90 39 273 2 Fragments arranged by size: 457 273 192 90 42 39 2 BsrBI CCG'CTC Cuts at: 0 195 1095 Size: 195 900 BsrFI r'CCGG_y Cuts at: 0 179 781 1095 Size: 179 602 314 Fragments arranged by size: 602 314 179 BssHII G'CGCG_C Cuts at: 0 22 1095 Size: 22 1073 BssSI C'ACGA_G Cuts at: 0 601 1095 Size: 601 494 BstAPI GCAn_nnn'nTGC Cuts at: 0 1058 1095 Size: 1058 37 BstYI r'GATC_y Cuts at: 0 567 1095 Size: 567 528 BtgI C'CryG_G Cuts at: 0 35 651 808 975 1095 Size: 35 616 157 167 120 Fragments arranged by size: 616 167 157 120 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 467 1095 Size: 467 628 BtsI GCAGTG_nn' Cuts at: 0 496 1063 1095 Size: 496 567 32 Fragments arranged by size: 567 496 32 EaeI y'GGCC_r Cuts at: 0 32 80 149 410 903 927 972 978 Size: 32 48 69 261 493 24 45 6 Cuts at: 978 1095 Size: 117 Fragments arranged by size: 493 261 117 69 48 45 32 24 6 EagI C'GGCC_G Cuts at: 0 80 149 1095 Size: 80 69 946 Fragments arranged by size: 946 80 69 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 205 211 337 490 748 842 1095 Size: 157 48 6 126 153 258 94 253 Fragments arranged by size: 258 253 157 153 126 94 48 6 EcoO109I rG'GnC_Cy Cuts at: 0 11 222 1010 1095 Size: 11 211 788 85 Fragments arranged by size: 788 211 85 11 EcoRI G'AATT_C Cuts at: 0 541 1095 Size: 541 554 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 369 1095 Size: 369 726 HaeII r_GCGC'y Cuts at: 0 303 444 703 1095 Size: 303 141 259 392 Fragments arranged by size: 392 303 259 141 KasI G'GCGC_C Cuts at: 0 699 1095 Size: 699 396 MscI TGG'CCA Cuts at: 0 412 1095 Size: 412 683 MslI CAynn'nnrTG Cuts at: 0 273 737 875 1095 Size: 273 464 138 220 Fragments arranged by size: 464 273 220 138 MspA1I CmG'CkG Cuts at: 0 18 37 129 204 846 925 977 1095 Size: 18 19 92 75 642 79 52 118 Fragments arranged by size: 642 118 92 79 75 52 19 18 NaeI GCC'GGC Cuts at: 0 783 1095 Size: 783 312 NarI GG'CG_CC Cuts at: 0 700 1095 Size: 700 395 NcoI C'CATG_G Cuts at: 0 651 1095 Size: 651 444 NgoMIV G'CCGG_C Cuts at: 0 781 1095 Size: 781 314 NotI GC'GGCC_GC Cuts at: 0 149 1095 Size: 149 946 NspI r_CATG'y Cuts at: 0 213 1095 Size: 213 882 PflMI CCAn_nnn'nTGG Cuts at: 0 714 1095 Size: 714 381 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 265 297 1095 Size: 265 32 798 Fragments arranged by size: 798 265 32 PpuMI rG'GwC_Cy Cuts at: 0 11 222 1095 Size: 11 211 873 Fragments arranged by size: 873 211 11 PspXI vC'TCGA_Gb Cuts at: 0 271 1095 Size: 271 824 SacII CC_GC'GG Cuts at: 0 38 978 1095 Size: 38 940 117 Fragments arranged by size: 940 117 38 SanDI GG'GwC_CC Cuts at: 0 11 222 1095 Size: 11 211 873 Fragments arranged by size: 873 211 11 SfoI GGC'GCC Cuts at: 0 701 1095 Size: 701 394 SmaI CCC'GGG Cuts at: 0 227 917 1095 Size: 227 690 178 Fragments arranged by size: 690 227 178 SmlI C'TyrA_G Cuts at: 0 271 343 514 751 1095 Size: 271 72 171 237 344 Fragments arranged by size: 344 271 237 171 72 StuI AGG'CCT Cuts at: 0 172 484 1095 Size: 172 312 611 Fragments arranged by size: 611 312 172 StyI C'CwwG_G Cuts at: 0 413 651 1095 Size: 413 238 444 Fragments arranged by size: 444 413 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 595 1095 Size: 595 500 TatI w'GTAC_w Cuts at: 0 518 1095 Size: 518 577 XhoI C'TCGA_G Cuts at: 0 271 1095 Size: 271 824 XmaI C'CCGG_G Cuts at: 0 225 915 1095 Size: 225 690 180 Fragments arranged by size: 690 225 180 Enzymes that do cut: AarI AccI AcuI AfeI AflII AleI AlfI ApaLI ApoI AvaI BanI BanII BbeI BcgI BclI BglI Bme1580I BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstYI BtgI BtgZI BtsI EaeI EagI Eco57MI EcoO109I EcoRI FalI HaeII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PflMI PpiI PpuMI PspXI SacII SanDI SfoI SmaI SmlI StuI StyI TaqII TatI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AflIII AgeI AhdI AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI XmnI ZraI